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Magnetic Tracking of Protein Synthesis in Microfluidic Environments—Challenges and Perspectives

A novel technique to study protein synthesis is proposed that uses magnetic nanoparticles in combination with microfluidic devices to achieve new insights into translational regulation. Cellular protein synthesis is an energy-demanding process which is tightly controlled and is dependent on environm...

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Autores principales: Wegener, Melanie, Ennen, Inga, Walhorn, Volker, Anselmetti, Dario, Hütten, Andreas, Dietz, Karl-Josef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523551/
https://www.ncbi.nlm.nih.gov/pubmed/30970646
http://dx.doi.org/10.3390/nano9040585
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author Wegener, Melanie
Ennen, Inga
Walhorn, Volker
Anselmetti, Dario
Hütten, Andreas
Dietz, Karl-Josef
author_facet Wegener, Melanie
Ennen, Inga
Walhorn, Volker
Anselmetti, Dario
Hütten, Andreas
Dietz, Karl-Josef
author_sort Wegener, Melanie
collection PubMed
description A novel technique to study protein synthesis is proposed that uses magnetic nanoparticles in combination with microfluidic devices to achieve new insights into translational regulation. Cellular protein synthesis is an energy-demanding process which is tightly controlled and is dependent on environmental and developmental requirements. Processivity and regulation of protein synthesis as part of the posttranslational nano-machinery has now moved back into the focus of cell biology, since it became apparent that multiple mechanisms are in place for fine-tuning of translation and conditional selection of transcripts. Recent methodological developments, such as ribosome foot printing, propel current research. Here we propose a strategy to open up a new field of labelling, separation, and analysis of specific polysomes using superparamagnetic particles following pharmacological arrest of translation during cell lysis and subsequent analysis. Translation occurs in polysomes, which are assemblies of specific transcripts, associated ribosomes, nascent polypeptides, and other factors. This supramolecular structure allows for unique approaches to selection of polysomes by targeting the specific transcript, ribosomes, or nascent polypeptides. Once labeled with functionalized superparamagnetic particles, such assemblies can be separated in microfluidic devices or magnetic ratchets and quantified. Insights into the dynamics of translation is obtained through quantifying large numbers of ribosomes along different locations of the polysome. Thus, an entire new concept for in vitro, ex vivo, and eventually single cell analysis will be realized and will allow for magnetic tracking of protein synthesis.
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spelling pubmed-65235512019-06-03 Magnetic Tracking of Protein Synthesis in Microfluidic Environments—Challenges and Perspectives Wegener, Melanie Ennen, Inga Walhorn, Volker Anselmetti, Dario Hütten, Andreas Dietz, Karl-Josef Nanomaterials (Basel) Perspective A novel technique to study protein synthesis is proposed that uses magnetic nanoparticles in combination with microfluidic devices to achieve new insights into translational regulation. Cellular protein synthesis is an energy-demanding process which is tightly controlled and is dependent on environmental and developmental requirements. Processivity and regulation of protein synthesis as part of the posttranslational nano-machinery has now moved back into the focus of cell biology, since it became apparent that multiple mechanisms are in place for fine-tuning of translation and conditional selection of transcripts. Recent methodological developments, such as ribosome foot printing, propel current research. Here we propose a strategy to open up a new field of labelling, separation, and analysis of specific polysomes using superparamagnetic particles following pharmacological arrest of translation during cell lysis and subsequent analysis. Translation occurs in polysomes, which are assemblies of specific transcripts, associated ribosomes, nascent polypeptides, and other factors. This supramolecular structure allows for unique approaches to selection of polysomes by targeting the specific transcript, ribosomes, or nascent polypeptides. Once labeled with functionalized superparamagnetic particles, such assemblies can be separated in microfluidic devices or magnetic ratchets and quantified. Insights into the dynamics of translation is obtained through quantifying large numbers of ribosomes along different locations of the polysome. Thus, an entire new concept for in vitro, ex vivo, and eventually single cell analysis will be realized and will allow for magnetic tracking of protein synthesis. MDPI 2019-04-09 /pmc/articles/PMC6523551/ /pubmed/30970646 http://dx.doi.org/10.3390/nano9040585 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Perspective
Wegener, Melanie
Ennen, Inga
Walhorn, Volker
Anselmetti, Dario
Hütten, Andreas
Dietz, Karl-Josef
Magnetic Tracking of Protein Synthesis in Microfluidic Environments—Challenges and Perspectives
title Magnetic Tracking of Protein Synthesis in Microfluidic Environments—Challenges and Perspectives
title_full Magnetic Tracking of Protein Synthesis in Microfluidic Environments—Challenges and Perspectives
title_fullStr Magnetic Tracking of Protein Synthesis in Microfluidic Environments—Challenges and Perspectives
title_full_unstemmed Magnetic Tracking of Protein Synthesis in Microfluidic Environments—Challenges and Perspectives
title_short Magnetic Tracking of Protein Synthesis in Microfluidic Environments—Challenges and Perspectives
title_sort magnetic tracking of protein synthesis in microfluidic environments—challenges and perspectives
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523551/
https://www.ncbi.nlm.nih.gov/pubmed/30970646
http://dx.doi.org/10.3390/nano9040585
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