Drying Enhances Signal Intensities for Global GC–MS Metabolomics

We report here that a straightforward change of the standard derivatization procedure for GC–MS metabolomics is leading to a strong increase in metabolite signal intensity. Drying samples between methoxymation and trimethylsilylation significantly increased signals by two- to tenfold in extracts of...

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Detalles Bibliográficos
Autores principales: Liebeke, Manuel, Puskás, Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523563/
https://www.ncbi.nlm.nih.gov/pubmed/30959834
http://dx.doi.org/10.3390/metabo9040068
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author Liebeke, Manuel
Puskás, Erik
author_facet Liebeke, Manuel
Puskás, Erik
author_sort Liebeke, Manuel
collection PubMed
description We report here that a straightforward change of the standard derivatization procedure for GC–MS metabolomics is leading to a strong increase in metabolite signal intensity. Drying samples between methoxymation and trimethylsilylation significantly increased signals by two- to tenfold in extracts of yeast cells, plant and animal tissue, and human urine. This easy step reduces the cost of sample material and the need for expensive new hardware.
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spelling pubmed-65235632019-06-03 Drying Enhances Signal Intensities for Global GC–MS Metabolomics Liebeke, Manuel Puskás, Erik Metabolites Communication We report here that a straightforward change of the standard derivatization procedure for GC–MS metabolomics is leading to a strong increase in metabolite signal intensity. Drying samples between methoxymation and trimethylsilylation significantly increased signals by two- to tenfold in extracts of yeast cells, plant and animal tissue, and human urine. This easy step reduces the cost of sample material and the need for expensive new hardware. MDPI 2019-04-05 /pmc/articles/PMC6523563/ /pubmed/30959834 http://dx.doi.org/10.3390/metabo9040068 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Liebeke, Manuel
Puskás, Erik
Drying Enhances Signal Intensities for Global GC–MS Metabolomics
title Drying Enhances Signal Intensities for Global GC–MS Metabolomics
title_full Drying Enhances Signal Intensities for Global GC–MS Metabolomics
title_fullStr Drying Enhances Signal Intensities for Global GC–MS Metabolomics
title_full_unstemmed Drying Enhances Signal Intensities for Global GC–MS Metabolomics
title_short Drying Enhances Signal Intensities for Global GC–MS Metabolomics
title_sort drying enhances signal intensities for global gc–ms metabolomics
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523563/
https://www.ncbi.nlm.nih.gov/pubmed/30959834
http://dx.doi.org/10.3390/metabo9040068
work_keys_str_mv AT liebekemanuel dryingenhancessignalintensitiesforglobalgcmsmetabolomics
AT puskaserik dryingenhancessignalintensitiesforglobalgcmsmetabolomics

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