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Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation

Due to the morphological resemblance between the electrospun nanofibers and extracellular matrix (ECM), electrospun fibers have been widely used to fabricate scaffolds for tissue regeneration. Relationships between scaffold morphologies and cells are cell type dependent. In this study, we sought to...

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Autores principales: Han, Dae Geun, Ahn, Chi Bum, Lee, Ji-Hyun, Hwang, Yongsung, Kim, Joo Hyun, Park, Kook Yang, Lee, Jin Woo, Son, Kuk Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523610/
https://www.ncbi.nlm.nih.gov/pubmed/30970611
http://dx.doi.org/10.3390/polym11040643
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author Han, Dae Geun
Ahn, Chi Bum
Lee, Ji-Hyun
Hwang, Yongsung
Kim, Joo Hyun
Park, Kook Yang
Lee, Jin Woo
Son, Kuk Hui
author_facet Han, Dae Geun
Ahn, Chi Bum
Lee, Ji-Hyun
Hwang, Yongsung
Kim, Joo Hyun
Park, Kook Yang
Lee, Jin Woo
Son, Kuk Hui
author_sort Han, Dae Geun
collection PubMed
description Due to the morphological resemblance between the electrospun nanofibers and extracellular matrix (ECM), electrospun fibers have been widely used to fabricate scaffolds for tissue regeneration. Relationships between scaffold morphologies and cells are cell type dependent. In this study, we sought to determine an optimum electrospun fiber diameter for human vascular smooth muscle cell (VSMC) regeneration in vascular scaffolds. Scaffolds were produced using poly(caprolactone) (PCL) electrospun fiber diameters of 0.5, 0.7, 1, 2, 2.5, 5, 7 or 10 μm, and VSMC survivals, proliferations, infiltrations, and phenotypes were recorded after culturing cells on these scaffolds for one, four, seven, or 10 days. VSMC phenotypes and macrophage infiltrations into scaffolds were evaluated by implanting scaffolds subcutaneously in a mouse for seven, 14, or 28 days. We found that human VSMC survival was not dependent on the electrospun fiber diameter. In summary, increasing fiber diameter reduced VSMC proliferation, increased VSMC infiltration and increased macrophage infiltration and activation. Our results indicate that electrospun PCL fiber diameters of 7 or 10 µm are optimum in terms of VSMC infiltration and macrophage infiltration and activation, albeit at the expense of VSMC proliferation.
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spelling pubmed-65236102019-06-03 Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation Han, Dae Geun Ahn, Chi Bum Lee, Ji-Hyun Hwang, Yongsung Kim, Joo Hyun Park, Kook Yang Lee, Jin Woo Son, Kuk Hui Polymers (Basel) Article Due to the morphological resemblance between the electrospun nanofibers and extracellular matrix (ECM), electrospun fibers have been widely used to fabricate scaffolds for tissue regeneration. Relationships between scaffold morphologies and cells are cell type dependent. In this study, we sought to determine an optimum electrospun fiber diameter for human vascular smooth muscle cell (VSMC) regeneration in vascular scaffolds. Scaffolds were produced using poly(caprolactone) (PCL) electrospun fiber diameters of 0.5, 0.7, 1, 2, 2.5, 5, 7 or 10 μm, and VSMC survivals, proliferations, infiltrations, and phenotypes were recorded after culturing cells on these scaffolds for one, four, seven, or 10 days. VSMC phenotypes and macrophage infiltrations into scaffolds were evaluated by implanting scaffolds subcutaneously in a mouse for seven, 14, or 28 days. We found that human VSMC survival was not dependent on the electrospun fiber diameter. In summary, increasing fiber diameter reduced VSMC proliferation, increased VSMC infiltration and increased macrophage infiltration and activation. Our results indicate that electrospun PCL fiber diameters of 7 or 10 µm are optimum in terms of VSMC infiltration and macrophage infiltration and activation, albeit at the expense of VSMC proliferation. MDPI 2019-04-09 /pmc/articles/PMC6523610/ /pubmed/30970611 http://dx.doi.org/10.3390/polym11040643 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Han, Dae Geun
Ahn, Chi Bum
Lee, Ji-Hyun
Hwang, Yongsung
Kim, Joo Hyun
Park, Kook Yang
Lee, Jin Woo
Son, Kuk Hui
Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation
title Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation
title_full Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation
title_fullStr Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation
title_full_unstemmed Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation
title_short Optimization of Electrospun Poly(caprolactone) Fiber Diameter for Vascular Scaffolds to Maximize Smooth Muscle Cell Infiltration and Phenotype Modulation
title_sort optimization of electrospun poly(caprolactone) fiber diameter for vascular scaffolds to maximize smooth muscle cell infiltration and phenotype modulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523610/
https://www.ncbi.nlm.nih.gov/pubmed/30970611
http://dx.doi.org/10.3390/polym11040643
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