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Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model
This communication reports the first comparative study addressing the effects of both structural architecture and mechanical loading on human mesenchymal stem cells (hMSC) positioned at the interface of a 3D in vitro model composed of a nanofibre/hydrogel laminate composite. hMSC phenotype was affec...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523693/ https://www.ncbi.nlm.nih.gov/pubmed/30987078 http://dx.doi.org/10.3390/nano9040522 |
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author | Kumar, Deepak Cain, Stuart A. Bosworth, Lucy A. |
author_facet | Kumar, Deepak Cain, Stuart A. Bosworth, Lucy A. |
author_sort | Kumar, Deepak |
collection | PubMed |
description | This communication reports the first comparative study addressing the effects of both structural architecture and mechanical loading on human mesenchymal stem cells (hMSC) positioned at the interface of a 3D in vitro model composed of a nanofibre/hydrogel laminate composite. hMSC phenotype was affected by both stimuli over a seven-day period. Cells were orientated parallel to the underlying fibre direction irrespective of environment (electrospun 2D fibre sheet or laminate 2D sheet with collagen gel layer). Application of cyclical tensile force (5% strain, 1 Hz, 1 h per day) encouraged hMSCs to remain at the fibre/gel interface, whereas cells cultured in static conditions migrated from the interface to the upper hydrogel layer. Depending on the stimulus applied, hMSCs presented an up-regulation in gene expression, indicative of several cell lineages, with those cultured at the interface and physically stimulated expressing markers indicative of angiogenesis, osteogenesis, and tenogenesis. This study highlights the importance of developing biomaterial scaffolds with environmental cues to specifically drive cells towards the tissue intended for bioengineering. |
format | Online Article Text |
id | pubmed-6523693 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65236932019-06-03 Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model Kumar, Deepak Cain, Stuart A. Bosworth, Lucy A. Nanomaterials (Basel) Communication This communication reports the first comparative study addressing the effects of both structural architecture and mechanical loading on human mesenchymal stem cells (hMSC) positioned at the interface of a 3D in vitro model composed of a nanofibre/hydrogel laminate composite. hMSC phenotype was affected by both stimuli over a seven-day period. Cells were orientated parallel to the underlying fibre direction irrespective of environment (electrospun 2D fibre sheet or laminate 2D sheet with collagen gel layer). Application of cyclical tensile force (5% strain, 1 Hz, 1 h per day) encouraged hMSCs to remain at the fibre/gel interface, whereas cells cultured in static conditions migrated from the interface to the upper hydrogel layer. Depending on the stimulus applied, hMSCs presented an up-regulation in gene expression, indicative of several cell lineages, with those cultured at the interface and physically stimulated expressing markers indicative of angiogenesis, osteogenesis, and tenogenesis. This study highlights the importance of developing biomaterial scaffolds with environmental cues to specifically drive cells towards the tissue intended for bioengineering. MDPI 2019-04-03 /pmc/articles/PMC6523693/ /pubmed/30987078 http://dx.doi.org/10.3390/nano9040522 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Kumar, Deepak Cain, Stuart A. Bosworth, Lucy A. Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model |
title | Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model |
title_full | Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model |
title_fullStr | Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model |
title_full_unstemmed | Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model |
title_short | Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model |
title_sort | effect of topography and physical stimulus on hmsc phenotype using a 3d in vitro model |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523693/ https://www.ncbi.nlm.nih.gov/pubmed/30987078 http://dx.doi.org/10.3390/nano9040522 |
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