Cargando…

Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model

This communication reports the first comparative study addressing the effects of both structural architecture and mechanical loading on human mesenchymal stem cells (hMSC) positioned at the interface of a 3D in vitro model composed of a nanofibre/hydrogel laminate composite. hMSC phenotype was affec...

Descripción completa

Detalles Bibliográficos
Autores principales: Kumar, Deepak, Cain, Stuart A., Bosworth, Lucy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523693/
https://www.ncbi.nlm.nih.gov/pubmed/30987078
http://dx.doi.org/10.3390/nano9040522
_version_ 1783419393227620352
author Kumar, Deepak
Cain, Stuart A.
Bosworth, Lucy A.
author_facet Kumar, Deepak
Cain, Stuart A.
Bosworth, Lucy A.
author_sort Kumar, Deepak
collection PubMed
description This communication reports the first comparative study addressing the effects of both structural architecture and mechanical loading on human mesenchymal stem cells (hMSC) positioned at the interface of a 3D in vitro model composed of a nanofibre/hydrogel laminate composite. hMSC phenotype was affected by both stimuli over a seven-day period. Cells were orientated parallel to the underlying fibre direction irrespective of environment (electrospun 2D fibre sheet or laminate 2D sheet with collagen gel layer). Application of cyclical tensile force (5% strain, 1 Hz, 1 h per day) encouraged hMSCs to remain at the fibre/gel interface, whereas cells cultured in static conditions migrated from the interface to the upper hydrogel layer. Depending on the stimulus applied, hMSCs presented an up-regulation in gene expression, indicative of several cell lineages, with those cultured at the interface and physically stimulated expressing markers indicative of angiogenesis, osteogenesis, and tenogenesis. This study highlights the importance of developing biomaterial scaffolds with environmental cues to specifically drive cells towards the tissue intended for bioengineering.
format Online
Article
Text
id pubmed-6523693
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-65236932019-06-03 Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model Kumar, Deepak Cain, Stuart A. Bosworth, Lucy A. Nanomaterials (Basel) Communication This communication reports the first comparative study addressing the effects of both structural architecture and mechanical loading on human mesenchymal stem cells (hMSC) positioned at the interface of a 3D in vitro model composed of a nanofibre/hydrogel laminate composite. hMSC phenotype was affected by both stimuli over a seven-day period. Cells were orientated parallel to the underlying fibre direction irrespective of environment (electrospun 2D fibre sheet or laminate 2D sheet with collagen gel layer). Application of cyclical tensile force (5% strain, 1 Hz, 1 h per day) encouraged hMSCs to remain at the fibre/gel interface, whereas cells cultured in static conditions migrated from the interface to the upper hydrogel layer. Depending on the stimulus applied, hMSCs presented an up-regulation in gene expression, indicative of several cell lineages, with those cultured at the interface and physically stimulated expressing markers indicative of angiogenesis, osteogenesis, and tenogenesis. This study highlights the importance of developing biomaterial scaffolds with environmental cues to specifically drive cells towards the tissue intended for bioengineering. MDPI 2019-04-03 /pmc/articles/PMC6523693/ /pubmed/30987078 http://dx.doi.org/10.3390/nano9040522 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Kumar, Deepak
Cain, Stuart A.
Bosworth, Lucy A.
Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model
title Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model
title_full Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model
title_fullStr Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model
title_full_unstemmed Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model
title_short Effect of Topography and Physical Stimulus on hMSC Phenotype Using a 3D In Vitro Model
title_sort effect of topography and physical stimulus on hmsc phenotype using a 3d in vitro model
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523693/
https://www.ncbi.nlm.nih.gov/pubmed/30987078
http://dx.doi.org/10.3390/nano9040522
work_keys_str_mv AT kumardeepak effectoftopographyandphysicalstimulusonhmscphenotypeusinga3dinvitromodel
AT cainstuarta effectoftopographyandphysicalstimulusonhmscphenotypeusinga3dinvitromodel
AT bosworthlucya effectoftopographyandphysicalstimulusonhmscphenotypeusinga3dinvitromodel