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Comparative Transcriptomics Reveal Key Sheep (Ovis aries) Hypothalamus LncRNAs that Affect Reproduction

SIMPLE SUMMARY: The hypothalamus has an important role in sheep reproduction. In this study, the key long noncoding RNAs (lncRNAs) associated with sheep fecundity were detected and characterized using the RNA Sequencing technique in sheep hypothalami. The results indicated that several key lncRNAs m...

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Autores principales: Zhang, Zhuangbiao, Tang, Jishun, Di, Ran, Liu, Qiuyue, Wang, Xiangyu, Gan, Shangquan, Zhang, Xiaosheng, Zhang, Jinlong, Hu, Wenping, Chu, Mingxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523726/
https://www.ncbi.nlm.nih.gov/pubmed/30965601
http://dx.doi.org/10.3390/ani9040152
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author Zhang, Zhuangbiao
Tang, Jishun
Di, Ran
Liu, Qiuyue
Wang, Xiangyu
Gan, Shangquan
Zhang, Xiaosheng
Zhang, Jinlong
Hu, Wenping
Chu, Mingxing
author_facet Zhang, Zhuangbiao
Tang, Jishun
Di, Ran
Liu, Qiuyue
Wang, Xiangyu
Gan, Shangquan
Zhang, Xiaosheng
Zhang, Jinlong
Hu, Wenping
Chu, Mingxing
author_sort Zhang, Zhuangbiao
collection PubMed
description SIMPLE SUMMARY: The hypothalamus has an important role in sheep reproduction. In this study, the key long noncoding RNAs (lncRNAs) associated with sheep fecundity were detected and characterized using the RNA Sequencing technique in sheep hypothalami. The results indicated that several key lncRNAs may affect crucial reproductive processes by differentially influencing the expression of their target genes in polytocous sheep in the follicular phase (PF) vs. monotocous sheep in the follicular phase (MF) and in polytocous sheep in the luteal phase (PL) vs. monotocous sheep in the luteal phase (ML). These results provide an insight into the prolificacy mechanism in sheep without FecB mutation in terms of the hypothalamus. ABSTRACT: The diverse functions of long noncoding RNAs (lncRNAs), which execute their functions mainly through modulating the activities of their target genes, have been have been widely studied for many years (including a number of studies involving lncRNAs in the ovary and uterus). Herein, for the first time, we detect lncRNAs in sheep hypothalami with FecB++ through RNA Sequencing (RNA-Seq) and identify a number of known and novel lncRNAs, with 622 and 809 found to be differentially expressed in polytocous sheep in the follicular phase (PF) vs. monotocous sheep in the follicular phase (MF) and polytocous sheep in the luteal phase (PL) vs. monotocous sheep in the luteal phase (ML), respectively. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed based on the predicted target genes. The most highly enriched GO terms (at the molecular function level) included carbonyl reductase (NADPH), 15-hydroxyprostaglandin dehydrogenase (NADP+), and prostaglandin-E2 9-reductase activity in PF vs. MF, and phosphatidylinositol-3,5-bisphosphate binding in PL vs. ML was associated with sheep fecundity. Interestingly, the phenomena of valine, leucine, and isoleucine degradation in PL vs. ML, and valine, leucine, and isoleucine biosynthesis in PF vs. MF, were present. In addition, the interactome of lncRNA and its targets showed that MSTRG.26777 and its cis-targets ENSOARG00000013744, ENSOARG00000013700, and ENSOARG00000013777, and MSTRG.105228 and its target WNT7A may participate in the sheep reproductive process at the hypothalamus level. Significantly, MSTRG.95128 and its cis-target Forkhead box L1 (FOXG1) were shown to be upregulated in PF vs. MF but downregulated in PL vs. ML. All of these results may be attributed to discoveries of new candidate genes and pathways related to sheep reproduction, and they may provide new views for understanding sheep reproduction without the effects of the FecB mutation.
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spelling pubmed-65237262019-06-04 Comparative Transcriptomics Reveal Key Sheep (Ovis aries) Hypothalamus LncRNAs that Affect Reproduction Zhang, Zhuangbiao Tang, Jishun Di, Ran Liu, Qiuyue Wang, Xiangyu Gan, Shangquan Zhang, Xiaosheng Zhang, Jinlong Hu, Wenping Chu, Mingxing Animals (Basel) Article SIMPLE SUMMARY: The hypothalamus has an important role in sheep reproduction. In this study, the key long noncoding RNAs (lncRNAs) associated with sheep fecundity were detected and characterized using the RNA Sequencing technique in sheep hypothalami. The results indicated that several key lncRNAs may affect crucial reproductive processes by differentially influencing the expression of their target genes in polytocous sheep in the follicular phase (PF) vs. monotocous sheep in the follicular phase (MF) and in polytocous sheep in the luteal phase (PL) vs. monotocous sheep in the luteal phase (ML). These results provide an insight into the prolificacy mechanism in sheep without FecB mutation in terms of the hypothalamus. ABSTRACT: The diverse functions of long noncoding RNAs (lncRNAs), which execute their functions mainly through modulating the activities of their target genes, have been have been widely studied for many years (including a number of studies involving lncRNAs in the ovary and uterus). Herein, for the first time, we detect lncRNAs in sheep hypothalami with FecB++ through RNA Sequencing (RNA-Seq) and identify a number of known and novel lncRNAs, with 622 and 809 found to be differentially expressed in polytocous sheep in the follicular phase (PF) vs. monotocous sheep in the follicular phase (MF) and polytocous sheep in the luteal phase (PL) vs. monotocous sheep in the luteal phase (ML), respectively. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed based on the predicted target genes. The most highly enriched GO terms (at the molecular function level) included carbonyl reductase (NADPH), 15-hydroxyprostaglandin dehydrogenase (NADP+), and prostaglandin-E2 9-reductase activity in PF vs. MF, and phosphatidylinositol-3,5-bisphosphate binding in PL vs. ML was associated with sheep fecundity. Interestingly, the phenomena of valine, leucine, and isoleucine degradation in PL vs. ML, and valine, leucine, and isoleucine biosynthesis in PF vs. MF, were present. In addition, the interactome of lncRNA and its targets showed that MSTRG.26777 and its cis-targets ENSOARG00000013744, ENSOARG00000013700, and ENSOARG00000013777, and MSTRG.105228 and its target WNT7A may participate in the sheep reproductive process at the hypothalamus level. Significantly, MSTRG.95128 and its cis-target Forkhead box L1 (FOXG1) were shown to be upregulated in PF vs. MF but downregulated in PL vs. ML. All of these results may be attributed to discoveries of new candidate genes and pathways related to sheep reproduction, and they may provide new views for understanding sheep reproduction without the effects of the FecB mutation. MDPI 2019-04-08 /pmc/articles/PMC6523726/ /pubmed/30965601 http://dx.doi.org/10.3390/ani9040152 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Zhuangbiao
Tang, Jishun
Di, Ran
Liu, Qiuyue
Wang, Xiangyu
Gan, Shangquan
Zhang, Xiaosheng
Zhang, Jinlong
Hu, Wenping
Chu, Mingxing
Comparative Transcriptomics Reveal Key Sheep (Ovis aries) Hypothalamus LncRNAs that Affect Reproduction
title Comparative Transcriptomics Reveal Key Sheep (Ovis aries) Hypothalamus LncRNAs that Affect Reproduction
title_full Comparative Transcriptomics Reveal Key Sheep (Ovis aries) Hypothalamus LncRNAs that Affect Reproduction
title_fullStr Comparative Transcriptomics Reveal Key Sheep (Ovis aries) Hypothalamus LncRNAs that Affect Reproduction
title_full_unstemmed Comparative Transcriptomics Reveal Key Sheep (Ovis aries) Hypothalamus LncRNAs that Affect Reproduction
title_short Comparative Transcriptomics Reveal Key Sheep (Ovis aries) Hypothalamus LncRNAs that Affect Reproduction
title_sort comparative transcriptomics reveal key sheep (ovis aries) hypothalamus lncrnas that affect reproduction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523726/
https://www.ncbi.nlm.nih.gov/pubmed/30965601
http://dx.doi.org/10.3390/ani9040152
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