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Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution
Plant genome evolution can be very complex and challenging to describe, even within a genus. Mechanisms that underlie genome variation are complex and can include whole-genome duplications, gene duplication and/or loss, and, importantly, multiple chromosomal rearrangements. Lupins (Lupinus) diverged...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523792/ https://www.ncbi.nlm.nih.gov/pubmed/30939837 http://dx.doi.org/10.3390/genes10040259 |
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author | Susek, Karolina Bielski, Wojciech Czyż, Katarzyna B. Hasterok, Robert Jackson, Scott A. Wolko, Bogdan Naganowska, Barbara |
author_facet | Susek, Karolina Bielski, Wojciech Czyż, Katarzyna B. Hasterok, Robert Jackson, Scott A. Wolko, Bogdan Naganowska, Barbara |
author_sort | Susek, Karolina |
collection | PubMed |
description | Plant genome evolution can be very complex and challenging to describe, even within a genus. Mechanisms that underlie genome variation are complex and can include whole-genome duplications, gene duplication and/or loss, and, importantly, multiple chromosomal rearrangements. Lupins (Lupinus) diverged from other legumes approximately 60 mya. In contrast to New World lupins, Old World lupins show high variability not only for chromosome numbers (2n = 32–52), but also for the basic chromosome number (x = 5–9, 13) and genome size. The evolutionary basis that underlies the karyotype evolution in lupins remains unknown, as it has so far been impossible to identify individual chromosomes. To shed light on chromosome changes and evolution, we used comparative chromosome mapping among 11 Old World lupins, with Lupinus angustifolius as the reference species. We applied set of L. angustifolius-derived bacterial artificial chromosome clones for fluorescence in situ hybridization. We demonstrate that chromosome variations in the species analyzed might have arisen from multiple changes in chromosome structure and number. We hypothesize about lupin karyotype evolution through polyploidy and subsequent aneuploidy. Additionally, we have established a cytogenomic map of L. angustifolius along with chromosome markers that can be used for related species to further improve comparative studies of crops and wild lupins. |
format | Online Article Text |
id | pubmed-6523792 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-65237922019-06-03 Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution Susek, Karolina Bielski, Wojciech Czyż, Katarzyna B. Hasterok, Robert Jackson, Scott A. Wolko, Bogdan Naganowska, Barbara Genes (Basel) Article Plant genome evolution can be very complex and challenging to describe, even within a genus. Mechanisms that underlie genome variation are complex and can include whole-genome duplications, gene duplication and/or loss, and, importantly, multiple chromosomal rearrangements. Lupins (Lupinus) diverged from other legumes approximately 60 mya. In contrast to New World lupins, Old World lupins show high variability not only for chromosome numbers (2n = 32–52), but also for the basic chromosome number (x = 5–9, 13) and genome size. The evolutionary basis that underlies the karyotype evolution in lupins remains unknown, as it has so far been impossible to identify individual chromosomes. To shed light on chromosome changes and evolution, we used comparative chromosome mapping among 11 Old World lupins, with Lupinus angustifolius as the reference species. We applied set of L. angustifolius-derived bacterial artificial chromosome clones for fluorescence in situ hybridization. We demonstrate that chromosome variations in the species analyzed might have arisen from multiple changes in chromosome structure and number. We hypothesize about lupin karyotype evolution through polyploidy and subsequent aneuploidy. Additionally, we have established a cytogenomic map of L. angustifolius along with chromosome markers that can be used for related species to further improve comparative studies of crops and wild lupins. MDPI 2019-04-01 /pmc/articles/PMC6523792/ /pubmed/30939837 http://dx.doi.org/10.3390/genes10040259 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Susek, Karolina Bielski, Wojciech Czyż, Katarzyna B. Hasterok, Robert Jackson, Scott A. Wolko, Bogdan Naganowska, Barbara Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution |
title | Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution |
title_full | Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution |
title_fullStr | Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution |
title_full_unstemmed | Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution |
title_short | Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution |
title_sort | impact of chromosomal rearrangements on the interpretation of lupin karyotype evolution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523792/ https://www.ncbi.nlm.nih.gov/pubmed/30939837 http://dx.doi.org/10.3390/genes10040259 |
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