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Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution

Plant genome evolution can be very complex and challenging to describe, even within a genus. Mechanisms that underlie genome variation are complex and can include whole-genome duplications, gene duplication and/or loss, and, importantly, multiple chromosomal rearrangements. Lupins (Lupinus) diverged...

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Autores principales: Susek, Karolina, Bielski, Wojciech, Czyż, Katarzyna B., Hasterok, Robert, Jackson, Scott A., Wolko, Bogdan, Naganowska, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523792/
https://www.ncbi.nlm.nih.gov/pubmed/30939837
http://dx.doi.org/10.3390/genes10040259
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author Susek, Karolina
Bielski, Wojciech
Czyż, Katarzyna B.
Hasterok, Robert
Jackson, Scott A.
Wolko, Bogdan
Naganowska, Barbara
author_facet Susek, Karolina
Bielski, Wojciech
Czyż, Katarzyna B.
Hasterok, Robert
Jackson, Scott A.
Wolko, Bogdan
Naganowska, Barbara
author_sort Susek, Karolina
collection PubMed
description Plant genome evolution can be very complex and challenging to describe, even within a genus. Mechanisms that underlie genome variation are complex and can include whole-genome duplications, gene duplication and/or loss, and, importantly, multiple chromosomal rearrangements. Lupins (Lupinus) diverged from other legumes approximately 60 mya. In contrast to New World lupins, Old World lupins show high variability not only for chromosome numbers (2n = 32–52), but also for the basic chromosome number (x = 5–9, 13) and genome size. The evolutionary basis that underlies the karyotype evolution in lupins remains unknown, as it has so far been impossible to identify individual chromosomes. To shed light on chromosome changes and evolution, we used comparative chromosome mapping among 11 Old World lupins, with Lupinus angustifolius as the reference species. We applied set of L. angustifolius-derived bacterial artificial chromosome clones for fluorescence in situ hybridization. We demonstrate that chromosome variations in the species analyzed might have arisen from multiple changes in chromosome structure and number. We hypothesize about lupin karyotype evolution through polyploidy and subsequent aneuploidy. Additionally, we have established a cytogenomic map of L. angustifolius along with chromosome markers that can be used for related species to further improve comparative studies of crops and wild lupins.
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spelling pubmed-65237922019-06-03 Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution Susek, Karolina Bielski, Wojciech Czyż, Katarzyna B. Hasterok, Robert Jackson, Scott A. Wolko, Bogdan Naganowska, Barbara Genes (Basel) Article Plant genome evolution can be very complex and challenging to describe, even within a genus. Mechanisms that underlie genome variation are complex and can include whole-genome duplications, gene duplication and/or loss, and, importantly, multiple chromosomal rearrangements. Lupins (Lupinus) diverged from other legumes approximately 60 mya. In contrast to New World lupins, Old World lupins show high variability not only for chromosome numbers (2n = 32–52), but also for the basic chromosome number (x = 5–9, 13) and genome size. The evolutionary basis that underlies the karyotype evolution in lupins remains unknown, as it has so far been impossible to identify individual chromosomes. To shed light on chromosome changes and evolution, we used comparative chromosome mapping among 11 Old World lupins, with Lupinus angustifolius as the reference species. We applied set of L. angustifolius-derived bacterial artificial chromosome clones for fluorescence in situ hybridization. We demonstrate that chromosome variations in the species analyzed might have arisen from multiple changes in chromosome structure and number. We hypothesize about lupin karyotype evolution through polyploidy and subsequent aneuploidy. Additionally, we have established a cytogenomic map of L. angustifolius along with chromosome markers that can be used for related species to further improve comparative studies of crops and wild lupins. MDPI 2019-04-01 /pmc/articles/PMC6523792/ /pubmed/30939837 http://dx.doi.org/10.3390/genes10040259 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Susek, Karolina
Bielski, Wojciech
Czyż, Katarzyna B.
Hasterok, Robert
Jackson, Scott A.
Wolko, Bogdan
Naganowska, Barbara
Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution
title Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution
title_full Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution
title_fullStr Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution
title_full_unstemmed Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution
title_short Impact of Chromosomal Rearrangements on the Interpretation of Lupin Karyotype Evolution
title_sort impact of chromosomal rearrangements on the interpretation of lupin karyotype evolution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523792/
https://www.ncbi.nlm.nih.gov/pubmed/30939837
http://dx.doi.org/10.3390/genes10040259
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