Cargando…

Dermatopontin in Skeletal Muscle Extracellular Matrix Regulates Myogenesis

Dermatopontin (DPT) is an extensively distributed non-collagenous component of the extracellular matrix predominantly found in the dermis of the skin, and consequently expressed in several tissues. In this study, we explored the role of DPT in myogenesis and perceived that it enhances the cell adhes...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Taeyeon, Ahmad, Khurshid, Shaikh, Sibhghatulla, Jan, Arif Tasleem, Seo, Myung-Gi, Lee, Eun Ju, Choi, Inho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523808/
https://www.ncbi.nlm.nih.gov/pubmed/30970625
http://dx.doi.org/10.3390/cells8040332
_version_ 1783419420166586368
author Kim, Taeyeon
Ahmad, Khurshid
Shaikh, Sibhghatulla
Jan, Arif Tasleem
Seo, Myung-Gi
Lee, Eun Ju
Choi, Inho
author_facet Kim, Taeyeon
Ahmad, Khurshid
Shaikh, Sibhghatulla
Jan, Arif Tasleem
Seo, Myung-Gi
Lee, Eun Ju
Choi, Inho
author_sort Kim, Taeyeon
collection PubMed
description Dermatopontin (DPT) is an extensively distributed non-collagenous component of the extracellular matrix predominantly found in the dermis of the skin, and consequently expressed in several tissues. In this study, we explored the role of DPT in myogenesis and perceived that it enhances the cell adhesion, reduces the cell proliferation and promotes the myoblast differentiation in C2C12 cells. Our results reveal an inhibitory effect with fibronectin (FN) in myoblast differentiation. We also observed that DPT and fibromodulin (FMOD) regulate positively to each other and promote myogenic differentiation. We further predicted the 3D structure of DPT, which is as yet unknown, and validated it using state-of-the-art in silico tools. Furthermore, we explored the in-silico protein-protein interaction between DPT-FMOD, DPT-FN, and FMOD-FN, and perceived that the interaction between FMOD-FN is more robust than DPT-FMOD and DPT-FN. Taken together, our findings have determined the role of DPT at different stages of the myogenic process.
format Online
Article
Text
id pubmed-6523808
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-65238082019-06-03 Dermatopontin in Skeletal Muscle Extracellular Matrix Regulates Myogenesis Kim, Taeyeon Ahmad, Khurshid Shaikh, Sibhghatulla Jan, Arif Tasleem Seo, Myung-Gi Lee, Eun Ju Choi, Inho Cells Article Dermatopontin (DPT) is an extensively distributed non-collagenous component of the extracellular matrix predominantly found in the dermis of the skin, and consequently expressed in several tissues. In this study, we explored the role of DPT in myogenesis and perceived that it enhances the cell adhesion, reduces the cell proliferation and promotes the myoblast differentiation in C2C12 cells. Our results reveal an inhibitory effect with fibronectin (FN) in myoblast differentiation. We also observed that DPT and fibromodulin (FMOD) regulate positively to each other and promote myogenic differentiation. We further predicted the 3D structure of DPT, which is as yet unknown, and validated it using state-of-the-art in silico tools. Furthermore, we explored the in-silico protein-protein interaction between DPT-FMOD, DPT-FN, and FMOD-FN, and perceived that the interaction between FMOD-FN is more robust than DPT-FMOD and DPT-FN. Taken together, our findings have determined the role of DPT at different stages of the myogenic process. MDPI 2019-04-09 /pmc/articles/PMC6523808/ /pubmed/30970625 http://dx.doi.org/10.3390/cells8040332 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Taeyeon
Ahmad, Khurshid
Shaikh, Sibhghatulla
Jan, Arif Tasleem
Seo, Myung-Gi
Lee, Eun Ju
Choi, Inho
Dermatopontin in Skeletal Muscle Extracellular Matrix Regulates Myogenesis
title Dermatopontin in Skeletal Muscle Extracellular Matrix Regulates Myogenesis
title_full Dermatopontin in Skeletal Muscle Extracellular Matrix Regulates Myogenesis
title_fullStr Dermatopontin in Skeletal Muscle Extracellular Matrix Regulates Myogenesis
title_full_unstemmed Dermatopontin in Skeletal Muscle Extracellular Matrix Regulates Myogenesis
title_short Dermatopontin in Skeletal Muscle Extracellular Matrix Regulates Myogenesis
title_sort dermatopontin in skeletal muscle extracellular matrix regulates myogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523808/
https://www.ncbi.nlm.nih.gov/pubmed/30970625
http://dx.doi.org/10.3390/cells8040332
work_keys_str_mv AT kimtaeyeon dermatopontininskeletalmuscleextracellularmatrixregulatesmyogenesis
AT ahmadkhurshid dermatopontininskeletalmuscleextracellularmatrixregulatesmyogenesis
AT shaikhsibhghatulla dermatopontininskeletalmuscleextracellularmatrixregulatesmyogenesis
AT janariftasleem dermatopontininskeletalmuscleextracellularmatrixregulatesmyogenesis
AT seomyunggi dermatopontininskeletalmuscleextracellularmatrixregulatesmyogenesis
AT leeeunju dermatopontininskeletalmuscleextracellularmatrixregulatesmyogenesis
AT choiinho dermatopontininskeletalmuscleextracellularmatrixregulatesmyogenesis