Quantification of carbonate radical formation by the bicarbonate-dependent peroxidase activity of superoxide dismutase 1 using pyrogallol red bleaching

Carbonate radicals (CO(3)•(-)) are generated by the bicarbonate-dependent peroxidase activity of cytosolic superoxide dismutase (Cu,Zn-SOD, SOD-1). The present work explored the use of bleaching of pyrogallol red (PGR) dye to quantify the rate of CO(3)•(-) formation from bovine and human SOD-1 (bSOD...

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Detalles Bibliográficos
Autores principales: Figueroa, Juan David, Fuentes-Lemus, Eduardo, Dorta, Eva, Melin, Victoria, Cortés-Ríos, Javiera, Faúndez, Mario, Contreras, David, Denicola, Ana, Álvarez, Beatriz, Davies, Michael J., López-Alarcón, Camilo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Method Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523824/
https://www.ncbi.nlm.nih.gov/pubmed/31102971
http://dx.doi.org/10.1016/j.redox.2019.101207

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