Cargando…

Insights into Inflammatory Priming of Adipose-Derived Mesenchymal Stem Cells: Validation of Extracellular Vesicles-Embedded miRNA Reference Genes as A Crucial Step for Donor Selection

Mesenchymal stem cells (MSCs) are promising tools for cell-based therapies due to their homing to injury sites, where they secrete bioactive factors such as cytokines, lipids, and nucleic acids, either free or conveyed within extracellular vesicles (EVs). Depending on the local environment, MSCs’ th...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ragni, Enrico, De Luca, Paola, Perucca Orfei, Carlotta, Colombini, Alessandra, Viganò, Marco, Lugano, Gaia, Bollati, Valentina, de Girolamo, Laura
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523846/ https://www.ncbi.nlm.nih.gov/pubmed/31018576 http://dx.doi.org/10.3390/cells8040369

Ejemplares similares

Identification of miRNA Reference Genes in Extracellular Vesicles from Adipose Derived Mesenchymal Stem Cells for Studying Osteoarthritis
por: Ragni, Enrico, et al.
Publicado: (2019)

Interaction with hyaluronan matrix and miRNA cargo as contributors for in vitro potential of mesenchymal stem cell-derived extracellular vesicles in a model of human osteoarthritic synoviocytes
por: Ragni, Enrico, et al.
Publicado: (2019)

Cartilage Protective and Immunomodulatory Features of Osteoarthritis Synovial Fluid-Treated Adipose-Derived Mesenchymal Stem Cells Secreted Factors and Extracellular Vesicles-Embedded miRNAs
por: Ragni, Enrico, et al.
Publicado: (2021)

Autologous Microfragmented Adipose Tissue Reduces the Catabolic and Fibrosis Response in an In Vitro Model of Tendon Cell Inflammation
por: Viganò, Marco, et al.
Publicado: (2019)

Evaluation of Different Seeding Methods for Cell-Seeded Collagen Matrix-Supported Autologous Chondrocyte Transplantation
por: Viganò, Marco, et al.
Publicado: (2019)

Secreted Factors and EV-miRNAs Orchestrate the Healing Capacity of Adipose Mesenchymal Stem Cells for the Treatment of Knee Osteoarthritis
por: Ragni, Enrico, et al.
Publicado: (2020)

Inflammatory priming enhances mesenchymal stromal cell secretome potential as a clinical product for regenerative medicine approaches through secreted factors and EV-miRNAs: the example of joint disease
por: Ragni, Enrico, et al.
Publicado: (2020)

miRNA Reference Genes in Extracellular Vesicles Released from Amniotic Membrane-Derived Mesenchymal Stromal Cells
por: Ragni, Enrico, et al.
Publicado: (2020)

Adipose-Derived Mesenchymal Stromal Cells Treated with Interleukin 1 Beta Produced Chondro-Protective Vesicles Able to Fast Penetrate in Cartilage
por: Colombini, Alessandra, et al.
Publicado: (2021)

Endogenous Controls for the Evaluation of Osteoarthritis-Related miRNAs in Extracellular Vesicles from Bone-Marrow-Derived Mesenchymal Stromal Cells and the Impact of Osteoarthritis Synovial Fluid
por: Ragni, Enrico, et al.
Publicado: (2022)

miR-103a-3p and miR-22-5p Are Reliable Reference Genes in Extracellular Vesicles From Cartilage, Adipose Tissue, and Bone Marrow Cells
por: Ragni, Enrico, et al.
Publicado: (2021)

Amniotic membrane‐mesenchymal stromal cells secreted factors and extracellular vesicle‐miRNAs: Anti‐inflammatory and regenerative features for musculoskeletal tissues
por: Ragni, Enrico, et al.
Publicado: (2021)

Molecular Characterization of Secreted Factors and Extracellular Vesicles-Embedded miRNAs from Bone Marrow-Derived Mesenchymal Stromal Cells in Presence of Synovial Fluid from Osteoarthritis Patients
por: Ragni, Enrico, et al.
Publicado: (2022)

In Vitro Study of Extracellular Vesicles Migration in Cartilage-Derived Osteoarthritis Samples Using Real-Time Quantitative Multimodal Nonlinear Optics Imaging
por: Mortati, Leonardo, et al.
Publicado: (2020)

Characterization of Microfragmented Adipose Tissue Architecture, Mesenchymal Stromal Cell Content and Release of Paracrine Mediators
por: Ragni, Enrico, et al.
Publicado: (2022)

Joint Tissue Protective and Immune-Modulating miRNA Landscape of Mesenchymal Stromal Cell-Derived Extracellular Vesicles under Different Osteoarthritis-Mimicking Conditions
por: Ragni, Enrico, et al.
Publicado: (2022)

Secreted Factors and Extracellular Vesicles Account for the Immunomodulatory and Tissue Regenerative Properties of Bone-Marrow-Derived Mesenchymal Stromal Cells for Osteoarthritis
por: Ragni, Enrico, et al.
Publicado: (2022)

Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of Cell Seeding by the Design of Experiment
por: Perucca Orfei, Carlotta, et al.
Publicado: (2018)

In Vitro Induction of Tendon-Specific Markers in Tendon Cells, Adipose- and Bone Marrow-Derived Stem Cells is Dependent on TGFβ3, BMP-12 and Ascorbic Acid Stimulation
por: Perucca Orfei, Carlotta, et al.
Publicado: (2019)

A(2A) adenosine receptors are involved in the reparative response of tendon cells to pulsed electromagnetic fields
por: Colombini, Alessandra, et al.
Publicado: (2020)

Pulsed electromagnetic fields improve the healing process of Achilles tendinopathy: a pilot study in a rat model
por: Perucca Orfei, Carlotta, et al.
Publicado: (2020)

Treatment of Achilles Tendinopathy with Autologous Adipose-derived Stromal Vascular Fraction: Results of a Randomized Prospective Clinical Trial
por: de Girolamo, Laura, et al.
Publicado: (2016)

Vitamin D’s Effect on the Proliferation and Inflammation of Human Intervertebral Disc Cells in Relation to the Functional Vitamin D Receptor Gene FokI Polymorphism
por: De Luca, Paola, et al.
Publicado: (2018)

High-Throughput Gene and Protein Analysis Revealed the Response of Disc Cells to Vitamin D, Depending on the VDR FokI Variants
por: Colombini, Alessandra, et al.
Publicado: (2021)

Innovative Visualization and Quantification of Extracellular Vesicles Interaction with and Incorporation in Target Cells in 3D Microenvironments
por: Ragni, Enrico, et al.
Publicado: (2020)

Tissue-Protective and Anti-Inflammatory Landmark of PRP-Treated Mesenchymal Stromal Cells Secretome for Osteoarthritis
por: Ragni, Enrico, et al.
Publicado: (2022)

Human Diseased Articular Cartilage Contains a Mesenchymal Stem Cell-Like Population of Chondroprogenitors with Strong Immunomodulatory Responses
por: De Luca, Paola, et al.
Publicado: (2019)

Reliable Reference Genes for Gene Expression Assessment in Tendon-Derived Cells under Inflammatory and Pro-Fibrotic/Healing Stimuli
por: Ragni, Enrico, et al.
Publicado: (2019)

Different culture conditions affect the growth of human tendon stem/progenitor cells (TSPCs) within a mixed tendon cells (TCs) population
por: Viganò, M., et al.
Publicado: (2017)

High Levels of Circulating Type II Collagen Degradation Marker (CTx-II) Are Associated with Specific VDR Polymorphisms in Patients with Adult Vertebral Osteochondrosis
por: Cauci, Sabina, et al.
Publicado: (2017)

The roles and therapeutic potentialof mesenchymal stem/stromal cells and their extracellular vesicles in tendinopathies
por: Quintero, Daniel, et al.
Publicado: (2023)

Mesenchymal stem cells as therapeutic target of biophysical stimulation for the treatment of musculoskeletal disorders
por: Viganò, Marco, et al.
Publicado: (2016)

The Effect of Three Different Suture Anchors for Rotator Cuff Repair on Primary Cultures of Human Bone Marrow Mesenchymal Stem Cells
por: Thiébat, Gabriele, et al.
Publicado: (2018)

Superior Osteo-Inductive and Osteo-Conductive Properties of Trabecular Titanium vs. PEEK Scaffolds on Human Mesenchymal Stem Cells: A Proof of Concept for the Use of Fusion Cages
por: Ragni, Enrico, et al.
Publicado: (2021)

Dose-Related and Time-Dependent Development of Collagenase-Induced Tendinopathy in Rats
por: Perucca Orfei, Carlotta, et al.
Publicado: (2016)

Cell-based therapies have disease-modifying effects on osteoarthritis in animal models. A systematic review by the ESSKA Orthobiologic Initiative. Part 1: adipose tissue-derived cell-based injectable therapies
por: Perucca Orfei, Carlotta, et al.
Publicado: (2022)

Human Tendon Stem/Progenitor Cell Features and Functionality Are Highly Influenced by in vitro Culture Conditions
por: Perucca Orfei, Carlotta, et al.
Publicado: (2021)

Inflammatory priming with IL-1β promotes the immunomodulatory behavior of adipose derived stem cells
por: Colombini, Alessandra, et al.
Publicado: (2022)

Comparison of EV-free fraction, EVs, and total secretome of amniotic mesenchymal stromal cells for their immunomodulatory potential: a translational perspective
por: Papait, Andrea, et al.
Publicado: (2022)

Systematic review and meta-analysis on the use of human platelet lysate for mesenchymal stem cell cultures: comparison with fetal bovine serum and considerations on the production protocol
por: Palombella, Silvia, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_n5uhl2i67irn1icsali6u4841b