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Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against Aspergillus flavus—A Common Poultry Feed Contaminant

SIMPLE SUMMARY: Mycotoxicosis in poultry has been seriously damaging the poultry production in Pakistan, resulting in economic losses to the country. The present study may act as a preliminary step for exploring the effect of indigenously characterized potential probiotic lactobacilli on aflatoxin p...

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Autores principales: Azeem, Nimra, Nawaz, Muhammad, Anjum, Aftab Ahmad, Saeed, Shagufta, Sana, Saba, Mustafa, Amina, Yousuf, Muhammad Rizwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523852/
https://www.ncbi.nlm.nih.gov/pubmed/30991667
http://dx.doi.org/10.3390/ani9040166
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author Azeem, Nimra
Nawaz, Muhammad
Anjum, Aftab Ahmad
Saeed, Shagufta
Sana, Saba
Mustafa, Amina
Yousuf, Muhammad Rizwan
author_facet Azeem, Nimra
Nawaz, Muhammad
Anjum, Aftab Ahmad
Saeed, Shagufta
Sana, Saba
Mustafa, Amina
Yousuf, Muhammad Rizwan
author_sort Azeem, Nimra
collection PubMed
description SIMPLE SUMMARY: Mycotoxicosis in poultry has been seriously damaging the poultry production in Pakistan, resulting in economic losses to the country. The present study may act as a preliminary step for exploring the effect of indigenously characterized potential probiotic lactobacilli on aflatoxin production by Aspergillus flavus. The present study explored anti-fungal Lactobacillus strains. Further investigations revealed their in vitro aflatoxin binding and anti-aflatoxigenic capabilities. These findings demonstrated L. gallinarum PL 149 to be an effective binder of aflatoxin B1 which may be used as a biocontrol agent against A. flavus and aflatoxin B1 production. It may be further employed for aflatoxin binding in poultry gut after in vivo evaluations. ABSTRACT: Aflatoxin contamination in human food and animal feed is a threat to public safety. Aflatoxin B1 (AFB1) can be especially damaging to poultry production and consequently economic development of Pakistan. The present study assessed the in vitro binding of AFB1 by indigenously characterized probiotic lactobacilli. Six isolates (Lactobacillus gallinarum PDP 10, Lactobacillus reuetri FYP 38, Lactobacillus fermentum PDP 24, Lactobacillus gallinarum PL 53, Lactobacillus paracasei PL 120, and Lactobacillus gallinarum PL 149) were tested for activity against toxigenic Aspergillus flavus W-7.1 (AFB1 producer) by well diffusion assay. Only three isolates (PL 53, PL 120, and PL 149) had activity against A. flavus W-7.1. The ameliorative effect of these probiotic isolates on AFB1 production was determined by co-culturing fungus with lactobacilli for 12 days, followed by aflatoxin quantification by high-performance liquid chromatography. In vitro AFB1 binding capacities of lactobacilli were determined by their incubation with a standard amount of AFB1 in phosphate buffer saline at 37 °C for 2 h. AFB1 binding capacities of isolates ranged from 28–65%. Four isolates (PDP 10, PDP 24, PL 120, and PL 149) also ceased aflatoxin production completely, whereas PL 53 showed 55% reduction in AFB1 production as compared to control. The present study demonstrated Lactobacillus gallinarum PL 149 to be an effective candidate AFB1 binding agent against Aspergillus flavus. These findings further support the binding ability of lactic acid bacteria for dietary contaminants.
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spelling pubmed-65238522019-06-04 Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against Aspergillus flavus—A Common Poultry Feed Contaminant Azeem, Nimra Nawaz, Muhammad Anjum, Aftab Ahmad Saeed, Shagufta Sana, Saba Mustafa, Amina Yousuf, Muhammad Rizwan Animals (Basel) Article SIMPLE SUMMARY: Mycotoxicosis in poultry has been seriously damaging the poultry production in Pakistan, resulting in economic losses to the country. The present study may act as a preliminary step for exploring the effect of indigenously characterized potential probiotic lactobacilli on aflatoxin production by Aspergillus flavus. The present study explored anti-fungal Lactobacillus strains. Further investigations revealed their in vitro aflatoxin binding and anti-aflatoxigenic capabilities. These findings demonstrated L. gallinarum PL 149 to be an effective binder of aflatoxin B1 which may be used as a biocontrol agent against A. flavus and aflatoxin B1 production. It may be further employed for aflatoxin binding in poultry gut after in vivo evaluations. ABSTRACT: Aflatoxin contamination in human food and animal feed is a threat to public safety. Aflatoxin B1 (AFB1) can be especially damaging to poultry production and consequently economic development of Pakistan. The present study assessed the in vitro binding of AFB1 by indigenously characterized probiotic lactobacilli. Six isolates (Lactobacillus gallinarum PDP 10, Lactobacillus reuetri FYP 38, Lactobacillus fermentum PDP 24, Lactobacillus gallinarum PL 53, Lactobacillus paracasei PL 120, and Lactobacillus gallinarum PL 149) were tested for activity against toxigenic Aspergillus flavus W-7.1 (AFB1 producer) by well diffusion assay. Only three isolates (PL 53, PL 120, and PL 149) had activity against A. flavus W-7.1. The ameliorative effect of these probiotic isolates on AFB1 production was determined by co-culturing fungus with lactobacilli for 12 days, followed by aflatoxin quantification by high-performance liquid chromatography. In vitro AFB1 binding capacities of lactobacilli were determined by their incubation with a standard amount of AFB1 in phosphate buffer saline at 37 °C for 2 h. AFB1 binding capacities of isolates ranged from 28–65%. Four isolates (PDP 10, PDP 24, PL 120, and PL 149) also ceased aflatoxin production completely, whereas PL 53 showed 55% reduction in AFB1 production as compared to control. The present study demonstrated Lactobacillus gallinarum PL 149 to be an effective candidate AFB1 binding agent against Aspergillus flavus. These findings further support the binding ability of lactic acid bacteria for dietary contaminants. MDPI 2019-04-15 /pmc/articles/PMC6523852/ /pubmed/30991667 http://dx.doi.org/10.3390/ani9040166 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Azeem, Nimra
Nawaz, Muhammad
Anjum, Aftab Ahmad
Saeed, Shagufta
Sana, Saba
Mustafa, Amina
Yousuf, Muhammad Rizwan
Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against Aspergillus flavus—A Common Poultry Feed Contaminant
title Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against Aspergillus flavus—A Common Poultry Feed Contaminant
title_full Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against Aspergillus flavus—A Common Poultry Feed Contaminant
title_fullStr Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against Aspergillus flavus—A Common Poultry Feed Contaminant
title_full_unstemmed Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against Aspergillus flavus—A Common Poultry Feed Contaminant
title_short Activity and Anti-Aflatoxigenic Effect of Indigenously Characterized Probiotic Lactobacilli against Aspergillus flavus—A Common Poultry Feed Contaminant
title_sort activity and anti-aflatoxigenic effect of indigenously characterized probiotic lactobacilli against aspergillus flavus—a common poultry feed contaminant
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523852/
https://www.ncbi.nlm.nih.gov/pubmed/30991667
http://dx.doi.org/10.3390/ani9040166
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