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Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths

Clusters of biological cells play an important role in normal and disease states, such as in the release of insulin from pancreatic islets and in the enhanced spread of cancer by clusters of circulating tumor cells. We report a method to pattern cells into clusters having sizes correlated to the dim...

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Detalles Bibliográficos
Autores principales: Banovetz, Joseph T., Li, Min, Pagariya, Darshna, Kim, Sungu, Ganapathysubramanian, Baskar, Anand, Robbyn K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523886/
https://www.ncbi.nlm.nih.gov/pubmed/31018537
http://dx.doi.org/10.3390/mi10040271
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author Banovetz, Joseph T.
Li, Min
Pagariya, Darshna
Kim, Sungu
Ganapathysubramanian, Baskar
Anand, Robbyn K.
author_facet Banovetz, Joseph T.
Li, Min
Pagariya, Darshna
Kim, Sungu
Ganapathysubramanian, Baskar
Anand, Robbyn K.
author_sort Banovetz, Joseph T.
collection PubMed
description Clusters of biological cells play an important role in normal and disease states, such as in the release of insulin from pancreatic islets and in the enhanced spread of cancer by clusters of circulating tumor cells. We report a method to pattern cells into clusters having sizes correlated to the dimensions of each electrode in an array of wireless bipolar electrodes (BPEs). The cells are captured by dielectrophoresis (DEP), which confers selectivity, and patterns cells without the need for physical barriers or adhesive interactions that can alter cell function. Our findings demonstrate that this approach readily achieves fine control of cell cluster size over a broader range set by other experimental parameters. These parameters include the magnitude of the voltage applied externally to drive capture at the BPE array, the rate of fluid flow, and the time allowed for DEP-based cell capture. Therefore, the reported method is anticipated to allow the influence of cluster size on cell function to be more fully investigated.
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spelling pubmed-65238862019-06-03 Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths Banovetz, Joseph T. Li, Min Pagariya, Darshna Kim, Sungu Ganapathysubramanian, Baskar Anand, Robbyn K. Micromachines (Basel) Article Clusters of biological cells play an important role in normal and disease states, such as in the release of insulin from pancreatic islets and in the enhanced spread of cancer by clusters of circulating tumor cells. We report a method to pattern cells into clusters having sizes correlated to the dimensions of each electrode in an array of wireless bipolar electrodes (BPEs). The cells are captured by dielectrophoresis (DEP), which confers selectivity, and patterns cells without the need for physical barriers or adhesive interactions that can alter cell function. Our findings demonstrate that this approach readily achieves fine control of cell cluster size over a broader range set by other experimental parameters. These parameters include the magnitude of the voltage applied externally to drive capture at the BPE array, the rate of fluid flow, and the time allowed for DEP-based cell capture. Therefore, the reported method is anticipated to allow the influence of cluster size on cell function to be more fully investigated. MDPI 2019-04-23 /pmc/articles/PMC6523886/ /pubmed/31018537 http://dx.doi.org/10.3390/mi10040271 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Banovetz, Joseph T.
Li, Min
Pagariya, Darshna
Kim, Sungu
Ganapathysubramanian, Baskar
Anand, Robbyn K.
Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths
title Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths
title_full Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths
title_fullStr Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths
title_full_unstemmed Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths
title_short Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths
title_sort defining cell cluster size by dielectrophoretic capture at an array of wireless electrodes of several distinct lengths
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523886/
https://www.ncbi.nlm.nih.gov/pubmed/31018537
http://dx.doi.org/10.3390/mi10040271
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