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Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats

We examined the cytoprotective effect of quercetin via activator protein (AP-1) and the heat shock protein 70 (Hsp70) pathway against light-induced retinal degeneration in rats. Quercetin was administered intraperitoneally to Sprague-Dawley rats for seven days before light exposure to intense white...

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Autores principales: Koyama, Yasurou, Kaidzu, Sachiko, Kim, Yong-Chul, Matsuoka, Yotaro, Ishihara, Tomoe, Ohira, Akihiro, Tanito, Masaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523921/
https://www.ncbi.nlm.nih.gov/pubmed/30934771
http://dx.doi.org/10.3390/antiox8040079
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author Koyama, Yasurou
Kaidzu, Sachiko
Kim, Yong-Chul
Matsuoka, Yotaro
Ishihara, Tomoe
Ohira, Akihiro
Tanito, Masaki
author_facet Koyama, Yasurou
Kaidzu, Sachiko
Kim, Yong-Chul
Matsuoka, Yotaro
Ishihara, Tomoe
Ohira, Akihiro
Tanito, Masaki
author_sort Koyama, Yasurou
collection PubMed
description We examined the cytoprotective effect of quercetin via activator protein (AP-1) and the heat shock protein 70 (Hsp70) pathway against light-induced retinal degeneration in rats. Quercetin was administered intraperitoneally to Sprague-Dawley rats for seven days before light exposure to intense white fluorescent light (3000 lux) for 24 h. Light-induced retinal damage was determined by the number of rows of photoreceptor cell nuclei, the microstructures of the rod outer segments and retinal pigment epithelium, and terminal deoxynucleotidyl transferase (TdT)-mediated 2′-Deoxyuridine-5′-triphosphate (dUTP) nick end labeling. To elucidate the cytoprotective mechanism of quercetin, expression levels were measured in the rat retinas of 8-hydroxy-deoxyguanosine (8-OHdG), a marker of oxidative stress; Hsp70; and transcription factor AP-1 transcription activity. Pretreatment with quercetin inhibited light-induced photoreceptor cellular apoptosis and subsequent retinal degeneration in rats. 8-OHdG and Hsp70 protein expressions were up-regulated markedly by light exposure and suppressed by quercetin pretreatment. The results of an electrophoretic mobility shift assay showed that AP-1-binding activity was activated by light exposure, and binding of c-Fos and c-Jun, but not JunB, mediated the binding activity. Intraperitoneal administration of quercetin decreases photooxidative damage in the retina and mediates cytoprotection against light-induced photoreceptor cell degeneration in rats. Suppression of the heterodimeric combination of c-Jun and c-Fos proteins at the AP-1 binding site is highly involved in quercetin-mediated cytoprotection.
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spelling pubmed-65239212019-06-03 Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats Koyama, Yasurou Kaidzu, Sachiko Kim, Yong-Chul Matsuoka, Yotaro Ishihara, Tomoe Ohira, Akihiro Tanito, Masaki Antioxidants (Basel) Article We examined the cytoprotective effect of quercetin via activator protein (AP-1) and the heat shock protein 70 (Hsp70) pathway against light-induced retinal degeneration in rats. Quercetin was administered intraperitoneally to Sprague-Dawley rats for seven days before light exposure to intense white fluorescent light (3000 lux) for 24 h. Light-induced retinal damage was determined by the number of rows of photoreceptor cell nuclei, the microstructures of the rod outer segments and retinal pigment epithelium, and terminal deoxynucleotidyl transferase (TdT)-mediated 2′-Deoxyuridine-5′-triphosphate (dUTP) nick end labeling. To elucidate the cytoprotective mechanism of quercetin, expression levels were measured in the rat retinas of 8-hydroxy-deoxyguanosine (8-OHdG), a marker of oxidative stress; Hsp70; and transcription factor AP-1 transcription activity. Pretreatment with quercetin inhibited light-induced photoreceptor cellular apoptosis and subsequent retinal degeneration in rats. 8-OHdG and Hsp70 protein expressions were up-regulated markedly by light exposure and suppressed by quercetin pretreatment. The results of an electrophoretic mobility shift assay showed that AP-1-binding activity was activated by light exposure, and binding of c-Fos and c-Jun, but not JunB, mediated the binding activity. Intraperitoneal administration of quercetin decreases photooxidative damage in the retina and mediates cytoprotection against light-induced photoreceptor cell degeneration in rats. Suppression of the heterodimeric combination of c-Jun and c-Fos proteins at the AP-1 binding site is highly involved in quercetin-mediated cytoprotection. MDPI 2019-03-27 /pmc/articles/PMC6523921/ /pubmed/30934771 http://dx.doi.org/10.3390/antiox8040079 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Koyama, Yasurou
Kaidzu, Sachiko
Kim, Yong-Chul
Matsuoka, Yotaro
Ishihara, Tomoe
Ohira, Akihiro
Tanito, Masaki
Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats
title Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats
title_full Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats
title_fullStr Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats
title_full_unstemmed Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats
title_short Suppression of Light-Induced Retinal Degeneration by Quercetin via the AP-1 Pathway in Rats
title_sort suppression of light-induced retinal degeneration by quercetin via the ap-1 pathway in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523921/
https://www.ncbi.nlm.nih.gov/pubmed/30934771
http://dx.doi.org/10.3390/antiox8040079
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