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Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs

This study reports (for the first time) the staining ability of vital (0.4% trypan blue and 1% neutral red) and fluorescent (Hoechst 33258) dyes to differentiate between live and dead Schistosoma haematobium (S. haematobium) eggs in human urine samples. Since S. haematobium egg is important in disea...

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Autores principales: Forson, Peter O., Tetteh-Quarcoo, Patience B., Ahenkorah, John, Aryee, Robert, Okine, Esther N., Afutu, Emmanuel, Djameh, Georgina I., Agyapong, Jeffrey, Anang, Abraham K., Ayeh-Kumi, Patrick F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6524036/
https://www.ncbi.nlm.nih.gov/pubmed/31018598
http://dx.doi.org/10.3390/medsci7040064
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author Forson, Peter O.
Tetteh-Quarcoo, Patience B.
Ahenkorah, John
Aryee, Robert
Okine, Esther N.
Afutu, Emmanuel
Djameh, Georgina I.
Agyapong, Jeffrey
Anang, Abraham K.
Ayeh-Kumi, Patrick F.
author_facet Forson, Peter O.
Tetteh-Quarcoo, Patience B.
Ahenkorah, John
Aryee, Robert
Okine, Esther N.
Afutu, Emmanuel
Djameh, Georgina I.
Agyapong, Jeffrey
Anang, Abraham K.
Ayeh-Kumi, Patrick F.
author_sort Forson, Peter O.
collection PubMed
description This study reports (for the first time) the staining ability of vital (0.4% trypan blue and 1% neutral red) and fluorescent (Hoechst 33258) dyes to differentiate between live and dead Schistosoma haematobium (S. haematobium) eggs in human urine samples. Since S. haematobium egg is important in disease pathology, diagnosis, transmission, and drug development research, it is essential to be able to easily distinguish live eggs from dead ones. Staining is considered a way of enhancing the identification of live and dead eggs. Urine samples from school children were examined for the presence of S. haematobium eggs. Vital and fluorescent dyes were used to stain the samples that contained S. haematobium eggs, after which they were observed using light and fluorescent microscopes, respectively. The Hoechst 33258 provided a good staining outcome for differentiation between live and dead eggs, followed by 0.4% Trypan blue. Regarding the 1% neutral red stain, even though it provided some evidence of which egg was alive or dead, the distinction was not very clear; therefore, it could be useful when used in combination with other stains for egg viability determination. The benefits of this study will include assessing the effect of drugs on S. haematobium eggs in Schistosomiasis research.
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spelling pubmed-65240362019-06-04 Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs Forson, Peter O. Tetteh-Quarcoo, Patience B. Ahenkorah, John Aryee, Robert Okine, Esther N. Afutu, Emmanuel Djameh, Georgina I. Agyapong, Jeffrey Anang, Abraham K. Ayeh-Kumi, Patrick F. Med Sci (Basel) Article This study reports (for the first time) the staining ability of vital (0.4% trypan blue and 1% neutral red) and fluorescent (Hoechst 33258) dyes to differentiate between live and dead Schistosoma haematobium (S. haematobium) eggs in human urine samples. Since S. haematobium egg is important in disease pathology, diagnosis, transmission, and drug development research, it is essential to be able to easily distinguish live eggs from dead ones. Staining is considered a way of enhancing the identification of live and dead eggs. Urine samples from school children were examined for the presence of S. haematobium eggs. Vital and fluorescent dyes were used to stain the samples that contained S. haematobium eggs, after which they were observed using light and fluorescent microscopes, respectively. The Hoechst 33258 provided a good staining outcome for differentiation between live and dead eggs, followed by 0.4% Trypan blue. Regarding the 1% neutral red stain, even though it provided some evidence of which egg was alive or dead, the distinction was not very clear; therefore, it could be useful when used in combination with other stains for egg viability determination. The benefits of this study will include assessing the effect of drugs on S. haematobium eggs in Schistosomiasis research. MDPI 2019-04-23 /pmc/articles/PMC6524036/ /pubmed/31018598 http://dx.doi.org/10.3390/medsci7040064 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Forson, Peter O.
Tetteh-Quarcoo, Patience B.
Ahenkorah, John
Aryee, Robert
Okine, Esther N.
Afutu, Emmanuel
Djameh, Georgina I.
Agyapong, Jeffrey
Anang, Abraham K.
Ayeh-Kumi, Patrick F.
Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs
title Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs
title_full Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs
title_fullStr Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs
title_full_unstemmed Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs
title_short Ability of Vital and Fluorescent Staining in the Differentiation of Schistosoma haematobium Live and Dead Eggs
title_sort ability of vital and fluorescent staining in the differentiation of schistosoma haematobium live and dead eggs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6524036/
https://www.ncbi.nlm.nih.gov/pubmed/31018598
http://dx.doi.org/10.3390/medsci7040064
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