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Glucosinolate Distribution in the Aerial Parts of sel1-10, a Disruption Mutant of the Sulfate Transporter SULTR1;2, in Mature Arabidopsis thaliana Plants

Plants take up sulfur (S), an essential element for all organisms, as sulfate, which is mainly attributed to the function of SULTR1;2 in Arabidopsis. A disruption mutant of SULTR1;2, sel1-10, has been characterized with phenotypes similar to plants grown under sulfur deficiency (−S). Although the ef...

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Autores principales: Morikawa-Ichinose, Tomomi, Kim, Sun-Ju, Allahham, Alaa, Kawaguchi, Ryota, Maruyama-Nakashita, Akiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6524378/
https://www.ncbi.nlm.nih.gov/pubmed/30974830
http://dx.doi.org/10.3390/plants8040095
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author Morikawa-Ichinose, Tomomi
Kim, Sun-Ju
Allahham, Alaa
Kawaguchi, Ryota
Maruyama-Nakashita, Akiko
author_facet Morikawa-Ichinose, Tomomi
Kim, Sun-Ju
Allahham, Alaa
Kawaguchi, Ryota
Maruyama-Nakashita, Akiko
author_sort Morikawa-Ichinose, Tomomi
collection PubMed
description Plants take up sulfur (S), an essential element for all organisms, as sulfate, which is mainly attributed to the function of SULTR1;2 in Arabidopsis. A disruption mutant of SULTR1;2, sel1-10, has been characterized with phenotypes similar to plants grown under sulfur deficiency (−S). Although the effects of −S on S metabolism were well investigated in seedlings, no studies have been performed on mature Arabidopsis plants. To study further the effects of −S on S metabolism, we analyzed the accumulation and distribution of S-containing compounds in different parts of mature sel1-10 and of the wild-type (WT) plants grown under long-day conditions. While the levels of sulfate, cysteine, and glutathione were almost similar between sel1-10 and WT, levels of glucosinolates (GSLs) differed between them depending on the parts of the plant. GSLs levels in the leaves and stems were generally lower in sel1-10 than those in WT. However, sel1-10 seeds maintained similar levels of aliphatic GSLs to those in WT plants. GSL accumulation in reproductive tissues is likely to be prioritized even when sulfate supply is limited in sel1-10 for its role in S storage and plant defense.
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spelling pubmed-65243782019-06-05 Glucosinolate Distribution in the Aerial Parts of sel1-10, a Disruption Mutant of the Sulfate Transporter SULTR1;2, in Mature Arabidopsis thaliana Plants Morikawa-Ichinose, Tomomi Kim, Sun-Ju Allahham, Alaa Kawaguchi, Ryota Maruyama-Nakashita, Akiko Plants (Basel) Article Plants take up sulfur (S), an essential element for all organisms, as sulfate, which is mainly attributed to the function of SULTR1;2 in Arabidopsis. A disruption mutant of SULTR1;2, sel1-10, has been characterized with phenotypes similar to plants grown under sulfur deficiency (−S). Although the effects of −S on S metabolism were well investigated in seedlings, no studies have been performed on mature Arabidopsis plants. To study further the effects of −S on S metabolism, we analyzed the accumulation and distribution of S-containing compounds in different parts of mature sel1-10 and of the wild-type (WT) plants grown under long-day conditions. While the levels of sulfate, cysteine, and glutathione were almost similar between sel1-10 and WT, levels of glucosinolates (GSLs) differed between them depending on the parts of the plant. GSLs levels in the leaves and stems were generally lower in sel1-10 than those in WT. However, sel1-10 seeds maintained similar levels of aliphatic GSLs to those in WT plants. GSL accumulation in reproductive tissues is likely to be prioritized even when sulfate supply is limited in sel1-10 for its role in S storage and plant defense. MDPI 2019-04-10 /pmc/articles/PMC6524378/ /pubmed/30974830 http://dx.doi.org/10.3390/plants8040095 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Morikawa-Ichinose, Tomomi
Kim, Sun-Ju
Allahham, Alaa
Kawaguchi, Ryota
Maruyama-Nakashita, Akiko
Glucosinolate Distribution in the Aerial Parts of sel1-10, a Disruption Mutant of the Sulfate Transporter SULTR1;2, in Mature Arabidopsis thaliana Plants
title Glucosinolate Distribution in the Aerial Parts of sel1-10, a Disruption Mutant of the Sulfate Transporter SULTR1;2, in Mature Arabidopsis thaliana Plants
title_full Glucosinolate Distribution in the Aerial Parts of sel1-10, a Disruption Mutant of the Sulfate Transporter SULTR1;2, in Mature Arabidopsis thaliana Plants
title_fullStr Glucosinolate Distribution in the Aerial Parts of sel1-10, a Disruption Mutant of the Sulfate Transporter SULTR1;2, in Mature Arabidopsis thaliana Plants
title_full_unstemmed Glucosinolate Distribution in the Aerial Parts of sel1-10, a Disruption Mutant of the Sulfate Transporter SULTR1;2, in Mature Arabidopsis thaliana Plants
title_short Glucosinolate Distribution in the Aerial Parts of sel1-10, a Disruption Mutant of the Sulfate Transporter SULTR1;2, in Mature Arabidopsis thaliana Plants
title_sort glucosinolate distribution in the aerial parts of sel1-10, a disruption mutant of the sulfate transporter sultr1;2, in mature arabidopsis thaliana plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6524378/
https://www.ncbi.nlm.nih.gov/pubmed/30974830
http://dx.doi.org/10.3390/plants8040095
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