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P5CR1 protein expression and the effect of gene-silencing on lung adenocarcinoma

The present study aimed to investigate the expression of pyrroline-5-carboxylate reductase 1 (P5CR1) protein in lung adenocarcinoma and paracancerous tissues and to explore the effect of silencing the encoding gene PYCR1 on the proliferation, migration, invasion, and cisplatin sensitivity in lung ad...

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Autores principales: She, Yang, Mao, Aiyou, Li, Feng, Wei, Xiaobin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6524628/
https://www.ncbi.nlm.nih.gov/pubmed/31143549
http://dx.doi.org/10.7717/peerj.6934
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author She, Yang
Mao, Aiyou
Li, Feng
Wei, Xiaobin
author_facet She, Yang
Mao, Aiyou
Li, Feng
Wei, Xiaobin
author_sort She, Yang
collection PubMed
description The present study aimed to investigate the expression of pyrroline-5-carboxylate reductase 1 (P5CR1) protein in lung adenocarcinoma and paracancerous tissues and to explore the effect of silencing the encoding gene PYCR1 on the proliferation, migration, invasion, and cisplatin sensitivity in lung adenocarcinoma cells, thereby providing a novel therapeutic target for the treatment of the disease. Immunohistochemistry staining was used to detect the P5CR1 protein expression in lung adenocarcinoma and paracancerous tissues, and statistical analysis evaluated the correlation between P5CR1 protein expression and gender, age, tissue part, or pathological grade. The CCK8 assay was performed to detect the proliferation and cisplatin sensitivity, while the effect of PYCR1 on the migration and invasion of lung adenocarcinoma cells was detected by scratch test and transwell chamber assay. The findings demonstrated that the P5CR1 protein expression was significantly elevated in lung adenocarcinoma tissues and correlated with the pathological grade, whereas no significant correlation was established between the protein expression and gender, age, or tissue part. Furthermore, after PYCR1 gene silencing, the proliferation and invasion were significantly suppressed, while the sensitivity to cisplatin was significantly enhanced. Therefore, it can be speculated that the PYCR1 gene affects the biological behavior of lung adenocarcinoma and cisplatin resistance, serving as a potential therapeutic target for lung adenocarcinoma.
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spelling pubmed-65246282019-05-29 P5CR1 protein expression and the effect of gene-silencing on lung adenocarcinoma She, Yang Mao, Aiyou Li, Feng Wei, Xiaobin PeerJ Cell Biology The present study aimed to investigate the expression of pyrroline-5-carboxylate reductase 1 (P5CR1) protein in lung adenocarcinoma and paracancerous tissues and to explore the effect of silencing the encoding gene PYCR1 on the proliferation, migration, invasion, and cisplatin sensitivity in lung adenocarcinoma cells, thereby providing a novel therapeutic target for the treatment of the disease. Immunohistochemistry staining was used to detect the P5CR1 protein expression in lung adenocarcinoma and paracancerous tissues, and statistical analysis evaluated the correlation between P5CR1 protein expression and gender, age, tissue part, or pathological grade. The CCK8 assay was performed to detect the proliferation and cisplatin sensitivity, while the effect of PYCR1 on the migration and invasion of lung adenocarcinoma cells was detected by scratch test and transwell chamber assay. The findings demonstrated that the P5CR1 protein expression was significantly elevated in lung adenocarcinoma tissues and correlated with the pathological grade, whereas no significant correlation was established between the protein expression and gender, age, or tissue part. Furthermore, after PYCR1 gene silencing, the proliferation and invasion were significantly suppressed, while the sensitivity to cisplatin was significantly enhanced. Therefore, it can be speculated that the PYCR1 gene affects the biological behavior of lung adenocarcinoma and cisplatin resistance, serving as a potential therapeutic target for lung adenocarcinoma. PeerJ Inc. 2019-05-14 /pmc/articles/PMC6524628/ /pubmed/31143549 http://dx.doi.org/10.7717/peerj.6934 Text en © 2019 She et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Cell Biology
She, Yang
Mao, Aiyou
Li, Feng
Wei, Xiaobin
P5CR1 protein expression and the effect of gene-silencing on lung adenocarcinoma
title P5CR1 protein expression and the effect of gene-silencing on lung adenocarcinoma
title_full P5CR1 protein expression and the effect of gene-silencing on lung adenocarcinoma
title_fullStr P5CR1 protein expression and the effect of gene-silencing on lung adenocarcinoma
title_full_unstemmed P5CR1 protein expression and the effect of gene-silencing on lung adenocarcinoma
title_short P5CR1 protein expression and the effect of gene-silencing on lung adenocarcinoma
title_sort p5cr1 protein expression and the effect of gene-silencing on lung adenocarcinoma
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6524628/
https://www.ncbi.nlm.nih.gov/pubmed/31143549
http://dx.doi.org/10.7717/peerj.6934
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