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Determination of tobacco alkaloid enantiomers using reversed phase UPLC/MS/MS
Nʹ-Nitrosonornicotine (NNN), a carcinogenic tobacco-specific Nʹ-nitrosamine (TSNA), is on the FDA list of harmful and potentially harmful constituents (HPHCs). Nornicotine, a product of the demethylation of nicotine, is the immediate alkaloid precursor for NNN formation. Nicotine, nornicotine and NN...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525314/ https://www.ncbi.nlm.nih.gov/pubmed/31193304 http://dx.doi.org/10.1016/j.heliyon.2019.e01719 |
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author | Ji, Huihua Wu, Ying Fannin, Franklin Bush, Lowell |
author_facet | Ji, Huihua Wu, Ying Fannin, Franklin Bush, Lowell |
author_sort | Ji, Huihua |
collection | PubMed |
description | Nʹ-Nitrosonornicotine (NNN), a carcinogenic tobacco-specific Nʹ-nitrosamine (TSNA), is on the FDA list of harmful and potentially harmful constituents (HPHCs). Nornicotine, a product of the demethylation of nicotine, is the immediate alkaloid precursor for NNN formation. Nicotine, nornicotine and NNN are optically active. The accumulation of the isomers of nicotine, nornicotine, and NNN impacts their biological activity. In this paper, we report the determination of tobacco alkaloid enantiomers (including nicotine, nornicotine, anabasine, and anatabine) in samples of different tobacco lines using a reversed phase ultra-performance liquid chromatography-tandem mass spectrometer (UPLC/MS/MS) method. Current method demonstates excellent detection capability for all alkaloid enantiomers, with correlation coefficients (r(2)) > 0.996 within their linear dynamic ranges. The limit of detection (LOD) and limit of quantitation (LOQ) of all analytes are less than 10 ng/mL and 30 ng/mL, respectively. In addition, their recovery and coefficient of variation (CV%) are within 100–115% and 0.2–3.7%, respectively. The method validated in this paper is simple, fast, and sensitive for the quantification of alkaloid enantiomers in tobacco leaf and has been applied to investigations of tobacco alkaloid enantiomer ratios in different tobacco lines and tobacco products. |
format | Online Article Text |
id | pubmed-6525314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-65253142019-05-28 Determination of tobacco alkaloid enantiomers using reversed phase UPLC/MS/MS Ji, Huihua Wu, Ying Fannin, Franklin Bush, Lowell Heliyon Article Nʹ-Nitrosonornicotine (NNN), a carcinogenic tobacco-specific Nʹ-nitrosamine (TSNA), is on the FDA list of harmful and potentially harmful constituents (HPHCs). Nornicotine, a product of the demethylation of nicotine, is the immediate alkaloid precursor for NNN formation. Nicotine, nornicotine and NNN are optically active. The accumulation of the isomers of nicotine, nornicotine, and NNN impacts their biological activity. In this paper, we report the determination of tobacco alkaloid enantiomers (including nicotine, nornicotine, anabasine, and anatabine) in samples of different tobacco lines using a reversed phase ultra-performance liquid chromatography-tandem mass spectrometer (UPLC/MS/MS) method. Current method demonstates excellent detection capability for all alkaloid enantiomers, with correlation coefficients (r(2)) > 0.996 within their linear dynamic ranges. The limit of detection (LOD) and limit of quantitation (LOQ) of all analytes are less than 10 ng/mL and 30 ng/mL, respectively. In addition, their recovery and coefficient of variation (CV%) are within 100–115% and 0.2–3.7%, respectively. The method validated in this paper is simple, fast, and sensitive for the quantification of alkaloid enantiomers in tobacco leaf and has been applied to investigations of tobacco alkaloid enantiomer ratios in different tobacco lines and tobacco products. Elsevier 2019-05-15 /pmc/articles/PMC6525314/ /pubmed/31193304 http://dx.doi.org/10.1016/j.heliyon.2019.e01719 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Ji, Huihua Wu, Ying Fannin, Franklin Bush, Lowell Determination of tobacco alkaloid enantiomers using reversed phase UPLC/MS/MS |
title | Determination of tobacco alkaloid enantiomers using reversed phase UPLC/MS/MS |
title_full | Determination of tobacco alkaloid enantiomers using reversed phase UPLC/MS/MS |
title_fullStr | Determination of tobacco alkaloid enantiomers using reversed phase UPLC/MS/MS |
title_full_unstemmed | Determination of tobacco alkaloid enantiomers using reversed phase UPLC/MS/MS |
title_short | Determination of tobacco alkaloid enantiomers using reversed phase UPLC/MS/MS |
title_sort | determination of tobacco alkaloid enantiomers using reversed phase uplc/ms/ms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525314/ https://www.ncbi.nlm.nih.gov/pubmed/31193304 http://dx.doi.org/10.1016/j.heliyon.2019.e01719 |
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