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Response surface based optimization of laccase production from Bacillus sp. MSK-01 using fruit juice waste as an effective substrate

Laccases are multicopper oxidases containing four copper atoms per monomer distributed in three redox sites. Because of its tremendous applications in different areas, isolation of new laccases with wide range of industrial implementation. The present study focuses on the optimization of laccase pro...

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Detalles Bibliográficos
Autores principales: Sondhi, Sonica, Saini, Kiranjot
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525328/
https://www.ncbi.nlm.nih.gov/pubmed/31193314
http://dx.doi.org/10.1016/j.heliyon.2019.e01718
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author Sondhi, Sonica
Saini, Kiranjot
author_facet Sondhi, Sonica
Saini, Kiranjot
author_sort Sondhi, Sonica
collection PubMed
description Laccases are multicopper oxidases containing four copper atoms per monomer distributed in three redox sites. Because of its tremendous applications in different areas, isolation of new laccases with wide range of industrial implementation. The present study focuses on the optimization of laccase production from Bacillus sp. MSK-01 under solid state fermentation conditions using fruit juice waste as the substrate. MSKLAC was produced extracellularly by the bacteria. This laccase was able to oxidize ABTS and syringaldazine. Various nutritional and environmental factors were utilized for increasing the enzyme yield. Plackett Burman was used to study the influence of input parameters on laccase yield. Tween-80, initial moisture ratio and magnesium sulphate were the major influencing factor affecting laccase yield. Central composite design of RSM was used for the modelling of experiment. Desirability approach was used to optimize laccase yield. Maximum laccase yield of 1645 IUg(−1) was obtained when 0.55% of tween -80, 1:2.34 initial moisture ratio and 300μM magnesium sulphate was used. A 470 fold increase in the yield of laccase from unoptimized condition was obtained.
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spelling pubmed-65253282019-05-28 Response surface based optimization of laccase production from Bacillus sp. MSK-01 using fruit juice waste as an effective substrate Sondhi, Sonica Saini, Kiranjot Heliyon Article Laccases are multicopper oxidases containing four copper atoms per monomer distributed in three redox sites. Because of its tremendous applications in different areas, isolation of new laccases with wide range of industrial implementation. The present study focuses on the optimization of laccase production from Bacillus sp. MSK-01 under solid state fermentation conditions using fruit juice waste as the substrate. MSKLAC was produced extracellularly by the bacteria. This laccase was able to oxidize ABTS and syringaldazine. Various nutritional and environmental factors were utilized for increasing the enzyme yield. Plackett Burman was used to study the influence of input parameters on laccase yield. Tween-80, initial moisture ratio and magnesium sulphate were the major influencing factor affecting laccase yield. Central composite design of RSM was used for the modelling of experiment. Desirability approach was used to optimize laccase yield. Maximum laccase yield of 1645 IUg(−1) was obtained when 0.55% of tween -80, 1:2.34 initial moisture ratio and 300μM magnesium sulphate was used. A 470 fold increase in the yield of laccase from unoptimized condition was obtained. Elsevier 2019-05-16 /pmc/articles/PMC6525328/ /pubmed/31193314 http://dx.doi.org/10.1016/j.heliyon.2019.e01718 Text en © 2019 The Authors. Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Sondhi, Sonica
Saini, Kiranjot
Response surface based optimization of laccase production from Bacillus sp. MSK-01 using fruit juice waste as an effective substrate
title Response surface based optimization of laccase production from Bacillus sp. MSK-01 using fruit juice waste as an effective substrate
title_full Response surface based optimization of laccase production from Bacillus sp. MSK-01 using fruit juice waste as an effective substrate
title_fullStr Response surface based optimization of laccase production from Bacillus sp. MSK-01 using fruit juice waste as an effective substrate
title_full_unstemmed Response surface based optimization of laccase production from Bacillus sp. MSK-01 using fruit juice waste as an effective substrate
title_short Response surface based optimization of laccase production from Bacillus sp. MSK-01 using fruit juice waste as an effective substrate
title_sort response surface based optimization of laccase production from bacillus sp. msk-01 using fruit juice waste as an effective substrate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525328/
https://www.ncbi.nlm.nih.gov/pubmed/31193314
http://dx.doi.org/10.1016/j.heliyon.2019.e01718
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