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Aquaporin 1 is located on the intestinal basolateral membrane in Toxocara canis and might play a role in drug uptake

BACKGROUND: Aquaporins (AQPs) are a family of integral membrane channel proteins that facilitate the transport of water and other small solutes across cell membranes. AQPs appear to play crucial roles in parasite survival and represent possible drug targets for novel intervention strategy. In this w...

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Autores principales: Ma, Guangxu, Jiang, Aiyun, Luo, Yongfang, Luo, Yongli, Huang, Hancheng, Zhou, Rongqiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525457/
https://www.ncbi.nlm.nih.gov/pubmed/31101125
http://dx.doi.org/10.1186/s13071-019-3500-1
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author Ma, Guangxu
Jiang, Aiyun
Luo, Yongfang
Luo, Yongli
Huang, Hancheng
Zhou, Rongqiong
author_facet Ma, Guangxu
Jiang, Aiyun
Luo, Yongfang
Luo, Yongli
Huang, Hancheng
Zhou, Rongqiong
author_sort Ma, Guangxu
collection PubMed
description BACKGROUND: Aquaporins (AQPs) are a family of integral membrane channel proteins that facilitate the transport of water and other small solutes across cell membranes. AQPs appear to play crucial roles in parasite survival and represent possible drug targets for novel intervention strategy. In this work, we investigated the tissue distribution and biological roles of an aquaporin TcAQP1 in the neglected parasitic nematode Toxocara canis. METHODS: Recombinant C-terminal hydrophilic domain of AQP1 of T. canis (rTcAQP1c) and polyclonal antibody against rTcAQP1c were produced to analyse the tissue expression of native TcAQP1 in adult (female and male) worms using an immunohistochemical approach. RNA interference (RNAi), quantitative real-time PCR (qRT-PCR) and nematocidal assays were performed to investigate the functional roles of TcAQP1 in the adult stage of T. canis. RESULTS: Immunofluorescence analysis showed that TcAQP1 was localised predominantly in the epithelial linings of the reproductive tract and basolateral membrane of the intestine in the adult stage (female and male) of T. canis, indicating important roles in reproduction, nutrient absorption and/or osmoregulation. Treatment with silencing RNA for 24 h resulted in a significant reduction of Tc-aqp-1 mRNA level in adult T. canis, though no phenotypical change was observed. The efficient gene knockdown compromised the nematocidal activity of albendazole in vitro, suggesting the role of TcAQP1 in drug uptake. CONCLUSIONS: The findings of this study provide important information about tissue expression and functional roles of TcAQP1 protein in adult T. canis. Understanding the biological functions of this protein in other developmental stages of T. canis and related parasitic nematodes would contribute to the discovery of novel diagnostic or anthelmintic targets.
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spelling pubmed-65254572019-05-24 Aquaporin 1 is located on the intestinal basolateral membrane in Toxocara canis and might play a role in drug uptake Ma, Guangxu Jiang, Aiyun Luo, Yongfang Luo, Yongli Huang, Hancheng Zhou, Rongqiong Parasit Vectors Research BACKGROUND: Aquaporins (AQPs) are a family of integral membrane channel proteins that facilitate the transport of water and other small solutes across cell membranes. AQPs appear to play crucial roles in parasite survival and represent possible drug targets for novel intervention strategy. In this work, we investigated the tissue distribution and biological roles of an aquaporin TcAQP1 in the neglected parasitic nematode Toxocara canis. METHODS: Recombinant C-terminal hydrophilic domain of AQP1 of T. canis (rTcAQP1c) and polyclonal antibody against rTcAQP1c were produced to analyse the tissue expression of native TcAQP1 in adult (female and male) worms using an immunohistochemical approach. RNA interference (RNAi), quantitative real-time PCR (qRT-PCR) and nematocidal assays were performed to investigate the functional roles of TcAQP1 in the adult stage of T. canis. RESULTS: Immunofluorescence analysis showed that TcAQP1 was localised predominantly in the epithelial linings of the reproductive tract and basolateral membrane of the intestine in the adult stage (female and male) of T. canis, indicating important roles in reproduction, nutrient absorption and/or osmoregulation. Treatment with silencing RNA for 24 h resulted in a significant reduction of Tc-aqp-1 mRNA level in adult T. canis, though no phenotypical change was observed. The efficient gene knockdown compromised the nematocidal activity of albendazole in vitro, suggesting the role of TcAQP1 in drug uptake. CONCLUSIONS: The findings of this study provide important information about tissue expression and functional roles of TcAQP1 protein in adult T. canis. Understanding the biological functions of this protein in other developmental stages of T. canis and related parasitic nematodes would contribute to the discovery of novel diagnostic or anthelmintic targets. BioMed Central 2019-05-17 /pmc/articles/PMC6525457/ /pubmed/31101125 http://dx.doi.org/10.1186/s13071-019-3500-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ma, Guangxu
Jiang, Aiyun
Luo, Yongfang
Luo, Yongli
Huang, Hancheng
Zhou, Rongqiong
Aquaporin 1 is located on the intestinal basolateral membrane in Toxocara canis and might play a role in drug uptake
title Aquaporin 1 is located on the intestinal basolateral membrane in Toxocara canis and might play a role in drug uptake
title_full Aquaporin 1 is located on the intestinal basolateral membrane in Toxocara canis and might play a role in drug uptake
title_fullStr Aquaporin 1 is located on the intestinal basolateral membrane in Toxocara canis and might play a role in drug uptake
title_full_unstemmed Aquaporin 1 is located on the intestinal basolateral membrane in Toxocara canis and might play a role in drug uptake
title_short Aquaporin 1 is located on the intestinal basolateral membrane in Toxocara canis and might play a role in drug uptake
title_sort aquaporin 1 is located on the intestinal basolateral membrane in toxocara canis and might play a role in drug uptake
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6525457/
https://www.ncbi.nlm.nih.gov/pubmed/31101125
http://dx.doi.org/10.1186/s13071-019-3500-1
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