A novel plasmid carrying carbapenem-resistant gene bla(KPC-2) in Pseudomonas aeruginosa

A carbapenem-resistant Pseudomonas aeruginosa strain PA1011 (ST463) was isolated from a patient in a surgical intensive care unit. PCR detection showed that PA1011 carried the bla(KPC-2) gene. A plasmid was isolated and sequenced using the Illumina NextSeq 500 and PacBio RSII sequencing platforms. T...

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Detalles Bibliográficos
Autores principales: Hu, Yan-Yan, Wang, Qi, Sun, Qiao-Ling, Chen, Gong-Xiang, Zhang, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526029/
https://www.ncbi.nlm.nih.gov/pubmed/31190916
http://dx.doi.org/10.2147/IDR.S196390
Descripción
Sumario:A carbapenem-resistant Pseudomonas aeruginosa strain PA1011 (ST463) was isolated from a patient in a surgical intensive care unit. PCR detection showed that PA1011 carried the bla(KPC-2) gene. A plasmid was isolated and sequenced using the Illumina NextSeq 500 and PacBio RSII sequencing platforms. The plasmid was named pPA1011 and carried the carbapenem-resistant gene bla(KPC-2.) pPA1011 was a 62,793 bp in length with an average G+C content of 58.8%. It was identified as a novel plasmid and encoded a novel genetic environment of bla(KPC-2) gene (ΔIS6-Tn3-ISKpn8-bla(KPC-2)-ISKpn6-IS26).

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