A novel plasmid carrying carbapenem-resistant gene bla(KPC-2) in Pseudomonas aeruginosa

A carbapenem-resistant Pseudomonas aeruginosa strain PA1011 (ST463) was isolated from a patient in a surgical intensive care unit. PCR detection showed that PA1011 carried the bla(KPC-2) gene. A plasmid was isolated and sequenced using the Illumina NextSeq 500 and PacBio RSII sequencing platforms. T...

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Autores principales: Hu, Yan-Yan, Wang, Qi, Sun, Qiao-Ling, Chen, Gong-Xiang, Zhang, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526029/
https://www.ncbi.nlm.nih.gov/pubmed/31190916
http://dx.doi.org/10.2147/IDR.S196390
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author Hu, Yan-Yan
Wang, Qi
Sun, Qiao-Ling
Chen, Gong-Xiang
Zhang, Rong
author_facet Hu, Yan-Yan
Wang, Qi
Sun, Qiao-Ling
Chen, Gong-Xiang
Zhang, Rong
author_sort Hu, Yan-Yan
collection PubMed
description A carbapenem-resistant Pseudomonas aeruginosa strain PA1011 (ST463) was isolated from a patient in a surgical intensive care unit. PCR detection showed that PA1011 carried the bla(KPC-2) gene. A plasmid was isolated and sequenced using the Illumina NextSeq 500 and PacBio RSII sequencing platforms. The plasmid was named pPA1011 and carried the carbapenem-resistant gene bla(KPC-2.) pPA1011 was a 62,793 bp in length with an average G+C content of 58.8%. It was identified as a novel plasmid and encoded a novel genetic environment of bla(KPC-2) gene (ΔIS6-Tn3-ISKpn8-bla(KPC-2)-ISKpn6-IS26).
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spelling pubmed-65260292019-06-12 A novel plasmid carrying carbapenem-resistant gene bla(KPC-2) in Pseudomonas aeruginosa Hu, Yan-Yan Wang, Qi Sun, Qiao-Ling Chen, Gong-Xiang Zhang, Rong Infect Drug Resist Short Report A carbapenem-resistant Pseudomonas aeruginosa strain PA1011 (ST463) was isolated from a patient in a surgical intensive care unit. PCR detection showed that PA1011 carried the bla(KPC-2) gene. A plasmid was isolated and sequenced using the Illumina NextSeq 500 and PacBio RSII sequencing platforms. The plasmid was named pPA1011 and carried the carbapenem-resistant gene bla(KPC-2.) pPA1011 was a 62,793 bp in length with an average G+C content of 58.8%. It was identified as a novel plasmid and encoded a novel genetic environment of bla(KPC-2) gene (ΔIS6-Tn3-ISKpn8-bla(KPC-2)-ISKpn6-IS26). Dove 2019-05-14 /pmc/articles/PMC6526029/ /pubmed/31190916 http://dx.doi.org/10.2147/IDR.S196390 Text en © 2019 Hu et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Short Report
Hu, Yan-Yan
Wang, Qi
Sun, Qiao-Ling
Chen, Gong-Xiang
Zhang, Rong
A novel plasmid carrying carbapenem-resistant gene bla(KPC-2) in Pseudomonas aeruginosa
title A novel plasmid carrying carbapenem-resistant gene bla(KPC-2) in Pseudomonas aeruginosa
title_full A novel plasmid carrying carbapenem-resistant gene bla(KPC-2) in Pseudomonas aeruginosa
title_fullStr A novel plasmid carrying carbapenem-resistant gene bla(KPC-2) in Pseudomonas aeruginosa
title_full_unstemmed A novel plasmid carrying carbapenem-resistant gene bla(KPC-2) in Pseudomonas aeruginosa
title_short A novel plasmid carrying carbapenem-resistant gene bla(KPC-2) in Pseudomonas aeruginosa
title_sort novel plasmid carrying carbapenem-resistant gene bla(kpc-2) in pseudomonas aeruginosa
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526029/
https://www.ncbi.nlm.nih.gov/pubmed/31190916
http://dx.doi.org/10.2147/IDR.S196390
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