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Bacterial cell division is recognized by the septin cytoskeleton for restriction by autophagy

Septins are cytoskeletal proteins widely recognized for their role in eukaryotic cell division. Septins also assemble into cage-like structures that entrap cytosolic Shigella flexneri targeted to macroautophagy/autophagy. Although the Shigella septin cage was discovered ~10 y ago, how septins recogn...

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Autores principales: Krokowski, Sina, Mostowy, Serge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526865/
https://www.ncbi.nlm.nih.gov/pubmed/30857451
http://dx.doi.org/10.1080/15548627.2019.1586499
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author Krokowski, Sina
Mostowy, Serge
author_facet Krokowski, Sina
Mostowy, Serge
author_sort Krokowski, Sina
collection PubMed
description Septins are cytoskeletal proteins widely recognized for their role in eukaryotic cell division. Septins also assemble into cage-like structures that entrap cytosolic Shigella flexneri targeted to macroautophagy/autophagy. Although the Shigella septin cage was discovered ~10 y ago, how septins recognize Shigella was poorly understood. We found that septins are recruited to regions of micrometer-scale curvature presented by dividing bacterial cells, and cardiolipin (a curvature-specific phospholipid) promotes septin recruitment to these regions. Chemical manipulation of bacteria revealed that following recruitment, septins assemble into cages around growing bacterial cells. Once assembled, septin cages inhibit Shigella cell division by autophagy and fusion with lysosomes. Thus, recognition of dividing bacterial cells by the septin cytoskeleton targets intracellular pathogens to antibacterial autophagy.
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spelling pubmed-65268652019-05-29 Bacterial cell division is recognized by the septin cytoskeleton for restriction by autophagy Krokowski, Sina Mostowy, Serge Autophagy Commentary Septins are cytoskeletal proteins widely recognized for their role in eukaryotic cell division. Septins also assemble into cage-like structures that entrap cytosolic Shigella flexneri targeted to macroautophagy/autophagy. Although the Shigella septin cage was discovered ~10 y ago, how septins recognize Shigella was poorly understood. We found that septins are recruited to regions of micrometer-scale curvature presented by dividing bacterial cells, and cardiolipin (a curvature-specific phospholipid) promotes septin recruitment to these regions. Chemical manipulation of bacteria revealed that following recruitment, septins assemble into cages around growing bacterial cells. Once assembled, septin cages inhibit Shigella cell division by autophagy and fusion with lysosomes. Thus, recognition of dividing bacterial cells by the septin cytoskeleton targets intracellular pathogens to antibacterial autophagy. Taylor & Francis 2019-03-11 /pmc/articles/PMC6526865/ /pubmed/30857451 http://dx.doi.org/10.1080/15548627.2019.1586499 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Commentary
Krokowski, Sina
Mostowy, Serge
Bacterial cell division is recognized by the septin cytoskeleton for restriction by autophagy
title Bacterial cell division is recognized by the septin cytoskeleton for restriction by autophagy
title_full Bacterial cell division is recognized by the septin cytoskeleton for restriction by autophagy
title_fullStr Bacterial cell division is recognized by the septin cytoskeleton for restriction by autophagy
title_full_unstemmed Bacterial cell division is recognized by the septin cytoskeleton for restriction by autophagy
title_short Bacterial cell division is recognized by the septin cytoskeleton for restriction by autophagy
title_sort bacterial cell division is recognized by the septin cytoskeleton for restriction by autophagy
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526865/
https://www.ncbi.nlm.nih.gov/pubmed/30857451
http://dx.doi.org/10.1080/15548627.2019.1586499
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