Cargando…
Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging
Background: In recent years, non-invasive imaging technologies for early cancer detection have drawn worldwide attention. In this study, an antinucleolin aptamer, AS1411, was successfully conjugated to BODIPY-labeled chitosan and studied on T47D and HEK-293 cell lines. Methods: After conjugation of...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526928/ https://www.ncbi.nlm.nih.gov/pubmed/31190811 http://dx.doi.org/10.2147/IJN.S202561 |
_version_ | 1783419971269820416 |
---|---|
author | Taki, Setareh Ardestani, Mehdi Shafiee |
author_facet | Taki, Setareh Ardestani, Mehdi Shafiee |
author_sort | Taki, Setareh |
collection | PubMed |
description | Background: In recent years, non-invasive imaging technologies for early cancer detection have drawn worldwide attention. In this study, an antinucleolin aptamer, AS1411, was successfully conjugated to BODIPY-labeled chitosan and studied on T47D and HEK-293 cell lines. Methods: After conjugation of the aptamer to chitosan nanoparticles and purification, its structure was confirmed using atomic force microscopy (AFM), electrophoretic light scattering (ELS) and dynamic light scattering (DLS). Results of AFM, DLS and ELS of both conjugation and chitosan were compared for confirmation of conjugation. Conjugates were mixed with BODIPY FL fluorescent dye, purified and lyophilized. The labeled conjugate was characterized using Fourier-transform infrared spectroscopy, ultraviolet–visible spectroscopy, ELS and DLS. In vitro cellular uptake and cytotoxic effects of BODIPY-labeled chitosan–AS1411 aptamer conjugates were evaluated using the XTT assay on T47D and HEK-293 cells and flow cytometry on T47D cells. Results: The data showed that uptake of BODIPY-labeled chitosan–AS1411 aptamer conjugate was satisfactory. Moreover, there was no statistically significant cytotoxicity of the conjugate on either cell line. Conclusion: The outcomes confirmed the potential application of this new targeted imaging agent as a novel cancer diagnostic agent for molecular imaging. |
format | Online Article Text |
id | pubmed-6526928 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-65269282019-06-12 Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging Taki, Setareh Ardestani, Mehdi Shafiee Int J Nanomedicine Original Research Background: In recent years, non-invasive imaging technologies for early cancer detection have drawn worldwide attention. In this study, an antinucleolin aptamer, AS1411, was successfully conjugated to BODIPY-labeled chitosan and studied on T47D and HEK-293 cell lines. Methods: After conjugation of the aptamer to chitosan nanoparticles and purification, its structure was confirmed using atomic force microscopy (AFM), electrophoretic light scattering (ELS) and dynamic light scattering (DLS). Results of AFM, DLS and ELS of both conjugation and chitosan were compared for confirmation of conjugation. Conjugates were mixed with BODIPY FL fluorescent dye, purified and lyophilized. The labeled conjugate was characterized using Fourier-transform infrared spectroscopy, ultraviolet–visible spectroscopy, ELS and DLS. In vitro cellular uptake and cytotoxic effects of BODIPY-labeled chitosan–AS1411 aptamer conjugates were evaluated using the XTT assay on T47D and HEK-293 cells and flow cytometry on T47D cells. Results: The data showed that uptake of BODIPY-labeled chitosan–AS1411 aptamer conjugate was satisfactory. Moreover, there was no statistically significant cytotoxicity of the conjugate on either cell line. Conclusion: The outcomes confirmed the potential application of this new targeted imaging agent as a novel cancer diagnostic agent for molecular imaging. Dove 2019-05-15 /pmc/articles/PMC6526928/ /pubmed/31190811 http://dx.doi.org/10.2147/IJN.S202561 Text en © 2019 Taki and Ardestani. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Taki, Setareh Ardestani, Mehdi Shafiee Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging |
title | Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging |
title_full | Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging |
title_fullStr | Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging |
title_full_unstemmed | Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging |
title_short | Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging |
title_sort | novel nanosized as1411–chitosan–bodipy conjugate for molecular fluorescent imaging |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526928/ https://www.ncbi.nlm.nih.gov/pubmed/31190811 http://dx.doi.org/10.2147/IJN.S202561 |
work_keys_str_mv | AT takisetareh novelnanosizedas1411chitosanbodipyconjugateformolecularfluorescentimaging AT ardestanimehdishafiee novelnanosizedas1411chitosanbodipyconjugateformolecularfluorescentimaging |