Cargando…

Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging

Background: In recent years, non-invasive imaging technologies for early cancer detection have drawn worldwide attention. In this study, an antinucleolin aptamer, AS1411, was successfully conjugated to BODIPY-labeled chitosan and studied on T47D and HEK-293 cell lines. Methods: After conjugation of...

Descripción completa

Detalles Bibliográficos
Autores principales: Taki, Setareh, Ardestani, Mehdi Shafiee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526928/
https://www.ncbi.nlm.nih.gov/pubmed/31190811
http://dx.doi.org/10.2147/IJN.S202561
_version_ 1783419971269820416
author Taki, Setareh
Ardestani, Mehdi Shafiee
author_facet Taki, Setareh
Ardestani, Mehdi Shafiee
author_sort Taki, Setareh
collection PubMed
description Background: In recent years, non-invasive imaging technologies for early cancer detection have drawn worldwide attention. In this study, an antinucleolin aptamer, AS1411, was successfully conjugated to BODIPY-labeled chitosan and studied on T47D and HEK-293 cell lines. Methods: After conjugation of the aptamer to chitosan nanoparticles and purification, its structure was confirmed using atomic force microscopy (AFM), electrophoretic light scattering (ELS) and dynamic light scattering (DLS). Results of AFM, DLS and ELS of both conjugation and chitosan were compared for confirmation of conjugation. Conjugates were mixed with BODIPY FL fluorescent dye, purified and lyophilized. The labeled conjugate was characterized using Fourier-transform infrared spectroscopy, ultraviolet–visible spectroscopy, ELS and DLS. In vitro cellular uptake and cytotoxic effects of BODIPY-labeled chitosan–AS1411 aptamer conjugates were evaluated using the XTT assay on T47D and HEK-293 cells and flow cytometry on T47D cells. Results: The data showed that uptake of BODIPY-labeled chitosan–AS1411 aptamer conjugate was satisfactory. Moreover, there was no statistically significant cytotoxicity of the conjugate on either cell line. Conclusion: The outcomes confirmed the potential application of this new targeted imaging agent as a novel cancer diagnostic agent for molecular imaging.
format Online
Article
Text
id pubmed-6526928
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-65269282019-06-12 Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging Taki, Setareh Ardestani, Mehdi Shafiee Int J Nanomedicine Original Research Background: In recent years, non-invasive imaging technologies for early cancer detection have drawn worldwide attention. In this study, an antinucleolin aptamer, AS1411, was successfully conjugated to BODIPY-labeled chitosan and studied on T47D and HEK-293 cell lines. Methods: After conjugation of the aptamer to chitosan nanoparticles and purification, its structure was confirmed using atomic force microscopy (AFM), electrophoretic light scattering (ELS) and dynamic light scattering (DLS). Results of AFM, DLS and ELS of both conjugation and chitosan were compared for confirmation of conjugation. Conjugates were mixed with BODIPY FL fluorescent dye, purified and lyophilized. The labeled conjugate was characterized using Fourier-transform infrared spectroscopy, ultraviolet–visible spectroscopy, ELS and DLS. In vitro cellular uptake and cytotoxic effects of BODIPY-labeled chitosan–AS1411 aptamer conjugates were evaluated using the XTT assay on T47D and HEK-293 cells and flow cytometry on T47D cells. Results: The data showed that uptake of BODIPY-labeled chitosan–AS1411 aptamer conjugate was satisfactory. Moreover, there was no statistically significant cytotoxicity of the conjugate on either cell line. Conclusion: The outcomes confirmed the potential application of this new targeted imaging agent as a novel cancer diagnostic agent for molecular imaging. Dove 2019-05-15 /pmc/articles/PMC6526928/ /pubmed/31190811 http://dx.doi.org/10.2147/IJN.S202561 Text en © 2019 Taki and Ardestani. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Taki, Setareh
Ardestani, Mehdi Shafiee
Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging
title Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging
title_full Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging
title_fullStr Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging
title_full_unstemmed Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging
title_short Novel nanosized AS1411–chitosan–BODIPY conjugate for molecular fluorescent imaging
title_sort novel nanosized as1411–chitosan–bodipy conjugate for molecular fluorescent imaging
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526928/
https://www.ncbi.nlm.nih.gov/pubmed/31190811
http://dx.doi.org/10.2147/IJN.S202561
work_keys_str_mv AT takisetareh novelnanosizedas1411chitosanbodipyconjugateformolecularfluorescentimaging
AT ardestanimehdishafiee novelnanosizedas1411chitosanbodipyconjugateformolecularfluorescentimaging