Different construction strategies affected on the physiology of Pichia pastoris strains highly expressed lipase by transcriptional analysis of key genes

We demonstrated previously that expression of Rhizomucor miehei lipase (RML) in Pichia pastoris could be significantly increased by addition of gene propeptide, optimized signal peptide codons and manipulation of gene dosage. In this study, effects of various strategies on the protein synthesis and...

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Detalles Bibliográficos
Autores principales: Huang, Jinjin, Wang, Qing, Bu, Wei, Chen, Lingxiao, Yang, Zhen, Zheng, Weifa, Li, Ying, Li, Jilun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527059/
https://www.ncbi.nlm.nih.gov/pubmed/31079540
http://dx.doi.org/10.1080/21655979.2019.1614422

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527059/
https://www.ncbi.nlm.nih.gov/pubmed/31079540
http://dx.doi.org/10.1080/21655979.2019.1614422

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