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Cell organelle-based analysis of cell chirality
The maintenance of tight endothelial junctions requires the establishment of proper cell polarity, which includes not only the apicobasal and front-rear polarity but also the left-right (L-R) polarity. The cell possesses an intrinsic mechanism of orienting the L-R axis with respect to the other axes...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527183/ https://www.ncbi.nlm.nih.gov/pubmed/31143366 http://dx.doi.org/10.1080/19420889.2019.1605277 |
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author | Fan, Jie Zhang, Haokang Rahman, Tasnif Stanton, Diana N. Wan, Leo Q. |
author_facet | Fan, Jie Zhang, Haokang Rahman, Tasnif Stanton, Diana N. Wan, Leo Q. |
author_sort | Fan, Jie |
collection | PubMed |
description | The maintenance of tight endothelial junctions requires the establishment of proper cell polarity, which includes not only the apicobasal and front-rear polarity but also the left-right (L-R) polarity. The cell possesses an intrinsic mechanism of orienting the L-R axis with respect to the other axes, following a left-hand or right-hand rule, termed cell chirality. We have previously reported that endothelial cells exhibit a clockwise or rightward bias on ring-shaped micropatterns. Now we further characterize the chirality of individual endothelial cells on micropatterns by analyzing the L-R positioning of the cell centroid relative to the nucleus-centrosome axis. Our results show that the centroids of endothelial cells preferably polarized towards the right side of the nucleus-centrosome axis. This bias is consistent with cell chirality characterized by other methods. These results suggest that the positioning of cell organelles is intrinsically L-R biased inside individual cells. This L-R bias provides an opportunity for determining cell chirality in situ, even in vivo, without the limitations of using isolated cells in in vitro engineered platforms. |
format | Online Article Text |
id | pubmed-6527183 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-65271832019-05-29 Cell organelle-based analysis of cell chirality Fan, Jie Zhang, Haokang Rahman, Tasnif Stanton, Diana N. Wan, Leo Q. Commun Integr Biol Short Communication The maintenance of tight endothelial junctions requires the establishment of proper cell polarity, which includes not only the apicobasal and front-rear polarity but also the left-right (L-R) polarity. The cell possesses an intrinsic mechanism of orienting the L-R axis with respect to the other axes, following a left-hand or right-hand rule, termed cell chirality. We have previously reported that endothelial cells exhibit a clockwise or rightward bias on ring-shaped micropatterns. Now we further characterize the chirality of individual endothelial cells on micropatterns by analyzing the L-R positioning of the cell centroid relative to the nucleus-centrosome axis. Our results show that the centroids of endothelial cells preferably polarized towards the right side of the nucleus-centrosome axis. This bias is consistent with cell chirality characterized by other methods. These results suggest that the positioning of cell organelles is intrinsically L-R biased inside individual cells. This L-R bias provides an opportunity for determining cell chirality in situ, even in vivo, without the limitations of using isolated cells in in vitro engineered platforms. Taylor & Francis 2019-04-24 /pmc/articles/PMC6527183/ /pubmed/31143366 http://dx.doi.org/10.1080/19420889.2019.1605277 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Fan, Jie Zhang, Haokang Rahman, Tasnif Stanton, Diana N. Wan, Leo Q. Cell organelle-based analysis of cell chirality |
title | Cell organelle-based analysis of cell chirality |
title_full | Cell organelle-based analysis of cell chirality |
title_fullStr | Cell organelle-based analysis of cell chirality |
title_full_unstemmed | Cell organelle-based analysis of cell chirality |
title_short | Cell organelle-based analysis of cell chirality |
title_sort | cell organelle-based analysis of cell chirality |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527183/ https://www.ncbi.nlm.nih.gov/pubmed/31143366 http://dx.doi.org/10.1080/19420889.2019.1605277 |
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