Cargando…

Influence of own mother's milk and different proportions of formula on intestinal microbiota of very preterm newborns

OBJECTIVE: To determine the differences in preterm infants’ stool microbiota considering the use of exclusive own mother’s milk and formula in different proportions in the first 28 days of life. METHODS: The study included newborns with GA ≤ 32 weeks divided in 5 group according the feeding regimen:...

Descripción completa

Detalles Bibliográficos
Autores principales: Zanella, Adriana, Silveira, Rita C., Roesch, Luiz F. W., Corso, Andréa L., Dobbler, Priscila T., Mai, Volker, Procianoy, Renato S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527203/
https://www.ncbi.nlm.nih.gov/pubmed/31107919
http://dx.doi.org/10.1371/journal.pone.0217296
_version_ 1783420004174135296
author Zanella, Adriana
Silveira, Rita C.
Roesch, Luiz F. W.
Corso, Andréa L.
Dobbler, Priscila T.
Mai, Volker
Procianoy, Renato S.
author_facet Zanella, Adriana
Silveira, Rita C.
Roesch, Luiz F. W.
Corso, Andréa L.
Dobbler, Priscila T.
Mai, Volker
Procianoy, Renato S.
author_sort Zanella, Adriana
collection PubMed
description OBJECTIVE: To determine the differences in preterm infants’ stool microbiota considering the use of exclusive own mother’s milk and formula in different proportions in the first 28 days of life. METHODS: The study included newborns with GA ≤ 32 weeks divided in 5 group according the feeding regimen: 7 exclusive own mother’s milk, 8 exclusive preterm formula, 16 mixed feeding with >70% own mother’s milk, 16 mixed feeding with >70% preterm formula, and 15 mixed 50% own mother’s milk and preterm formula. Exclusion criteria: congenital infections, congenital malformations and newborns of drug addicted mothers. Stools were collected weekly during the first 28 days. Microbial DNA extraction, 16S rRNA amplification and sequencing were performed. RESULTS: All groups were similar in perinatal and neonatal data. There were significant differences in microbial community among treatments. Approximately 37% of the variation in distance between microbial communities was explained by use of exclusive own mother´s milk only compared to other diets. The diet composed by exclusive own mother´s milk allowed for greater microbial richness (average of 85 OTUs) while diets based on preferably formula, exclusive formula, preferably maternal milk, and mixed of formula and maternal milk presented an average of 9, 29, 23, and 25 OTUs respectively. The mean proportion of the genus Escherichia and Clostridium was always greater in those containing formula than in the those with maternal milk only. CONCLUSIONS: Fecal microbiota in the neonatal period of preterm infants fed with exclusive own mother’s milk presented increased richness and differences in microbial composition from those fed with different proportions of formula.
format Online
Article
Text
id pubmed-6527203
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-65272032019-06-05 Influence of own mother's milk and different proportions of formula on intestinal microbiota of very preterm newborns Zanella, Adriana Silveira, Rita C. Roesch, Luiz F. W. Corso, Andréa L. Dobbler, Priscila T. Mai, Volker Procianoy, Renato S. PLoS One Research Article OBJECTIVE: To determine the differences in preterm infants’ stool microbiota considering the use of exclusive own mother’s milk and formula in different proportions in the first 28 days of life. METHODS: The study included newborns with GA ≤ 32 weeks divided in 5 group according the feeding regimen: 7 exclusive own mother’s milk, 8 exclusive preterm formula, 16 mixed feeding with >70% own mother’s milk, 16 mixed feeding with >70% preterm formula, and 15 mixed 50% own mother’s milk and preterm formula. Exclusion criteria: congenital infections, congenital malformations and newborns of drug addicted mothers. Stools were collected weekly during the first 28 days. Microbial DNA extraction, 16S rRNA amplification and sequencing were performed. RESULTS: All groups were similar in perinatal and neonatal data. There were significant differences in microbial community among treatments. Approximately 37% of the variation in distance between microbial communities was explained by use of exclusive own mother´s milk only compared to other diets. The diet composed by exclusive own mother´s milk allowed for greater microbial richness (average of 85 OTUs) while diets based on preferably formula, exclusive formula, preferably maternal milk, and mixed of formula and maternal milk presented an average of 9, 29, 23, and 25 OTUs respectively. The mean proportion of the genus Escherichia and Clostridium was always greater in those containing formula than in the those with maternal milk only. CONCLUSIONS: Fecal microbiota in the neonatal period of preterm infants fed with exclusive own mother’s milk presented increased richness and differences in microbial composition from those fed with different proportions of formula. Public Library of Science 2019-05-20 /pmc/articles/PMC6527203/ /pubmed/31107919 http://dx.doi.org/10.1371/journal.pone.0217296 Text en © 2019 Zanella et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zanella, Adriana
Silveira, Rita C.
Roesch, Luiz F. W.
Corso, Andréa L.
Dobbler, Priscila T.
Mai, Volker
Procianoy, Renato S.
Influence of own mother's milk and different proportions of formula on intestinal microbiota of very preterm newborns
title Influence of own mother's milk and different proportions of formula on intestinal microbiota of very preterm newborns
title_full Influence of own mother's milk and different proportions of formula on intestinal microbiota of very preterm newborns
title_fullStr Influence of own mother's milk and different proportions of formula on intestinal microbiota of very preterm newborns
title_full_unstemmed Influence of own mother's milk and different proportions of formula on intestinal microbiota of very preterm newborns
title_short Influence of own mother's milk and different proportions of formula on intestinal microbiota of very preterm newborns
title_sort influence of own mother's milk and different proportions of formula on intestinal microbiota of very preterm newborns
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527203/
https://www.ncbi.nlm.nih.gov/pubmed/31107919
http://dx.doi.org/10.1371/journal.pone.0217296
work_keys_str_mv AT zanellaadriana influenceofownmothersmilkanddifferentproportionsofformulaonintestinalmicrobiotaofverypretermnewborns
AT silveiraritac influenceofownmothersmilkanddifferentproportionsofformulaonintestinalmicrobiotaofverypretermnewborns
AT roeschluizfw influenceofownmothersmilkanddifferentproportionsofformulaonintestinalmicrobiotaofverypretermnewborns
AT corsoandreal influenceofownmothersmilkanddifferentproportionsofformulaonintestinalmicrobiotaofverypretermnewborns
AT dobblerpriscilat influenceofownmothersmilkanddifferentproportionsofformulaonintestinalmicrobiotaofverypretermnewborns
AT maivolker influenceofownmothersmilkanddifferentproportionsofformulaonintestinalmicrobiotaofverypretermnewborns
AT procianoyrenatos influenceofownmothersmilkanddifferentproportionsofformulaonintestinalmicrobiotaofverypretermnewborns