The Netrin-4/Laminin γ1/Neogenin-1 complex mediates migration in SK-N-SH neuroblastoma cells

Neuroblastoma (NB) is the most common pediatric extracranial solid tumor. It arises during development of the sympathetic nervous system. Netrin-4 (NTN4), a laminin-related protein, has been proposed as a key factor to target NB metastasis, although there is controversy about its function. Here, we...

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Autores principales: Villanueva, Andrea A., Puvogel, Sofía, Lois, Pablo, Muñoz-Palma, Ernesto, Ramírez Orellana, Manuel, Lubieniecki, Fabiana, Casco Claro, Fernando, Gallegos, Iván, García-Castro, Javier, Sanchez-Gomez, Pilar, Torres, Vicente A., Palma, Verónica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527380/
https://www.ncbi.nlm.nih.gov/pubmed/30160193
http://dx.doi.org/10.1080/19336918.2018.1506652
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author Villanueva, Andrea A.
Puvogel, Sofía
Lois, Pablo
Muñoz-Palma, Ernesto
Ramírez Orellana, Manuel
Lubieniecki, Fabiana
Casco Claro, Fernando
Gallegos, Iván
García-Castro, Javier
Sanchez-Gomez, Pilar
Torres, Vicente A.
Palma, Verónica
author_facet Villanueva, Andrea A.
Puvogel, Sofía
Lois, Pablo
Muñoz-Palma, Ernesto
Ramírez Orellana, Manuel
Lubieniecki, Fabiana
Casco Claro, Fernando
Gallegos, Iván
García-Castro, Javier
Sanchez-Gomez, Pilar
Torres, Vicente A.
Palma, Verónica
author_sort Villanueva, Andrea A.
collection PubMed
description Neuroblastoma (NB) is the most common pediatric extracranial solid tumor. It arises during development of the sympathetic nervous system. Netrin-4 (NTN4), a laminin-related protein, has been proposed as a key factor to target NB metastasis, although there is controversy about its function. Here, we show that NTN4 is broadly expressed in tumor, stroma and blood vessels of NB patient samples. Furthermore, NTN4 was shown to act as a cell adhesion molecule required for the migration induced by Neogenin-1 (NEO1) in SK-N-SH neuroblastoma cells. Therefore, we propose that NTN4, by forming a ternary complex with Laminin γ1 (LMγ1) and NEO1, acts as an essential extracellular matrix component, which induces the migration of SK-N-SH cells.
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spelling pubmed-65273802019-05-31 The Netrin-4/Laminin γ1/Neogenin-1 complex mediates migration in SK-N-SH neuroblastoma cells Villanueva, Andrea A. Puvogel, Sofía Lois, Pablo Muñoz-Palma, Ernesto Ramírez Orellana, Manuel Lubieniecki, Fabiana Casco Claro, Fernando Gallegos, Iván García-Castro, Javier Sanchez-Gomez, Pilar Torres, Vicente A. Palma, Verónica Cell Adh Migr Short Communication Neuroblastoma (NB) is the most common pediatric extracranial solid tumor. It arises during development of the sympathetic nervous system. Netrin-4 (NTN4), a laminin-related protein, has been proposed as a key factor to target NB metastasis, although there is controversy about its function. Here, we show that NTN4 is broadly expressed in tumor, stroma and blood vessels of NB patient samples. Furthermore, NTN4 was shown to act as a cell adhesion molecule required for the migration induced by Neogenin-1 (NEO1) in SK-N-SH neuroblastoma cells. Therefore, we propose that NTN4, by forming a ternary complex with Laminin γ1 (LMγ1) and NEO1, acts as an essential extracellular matrix component, which induces the migration of SK-N-SH cells. Taylor & Francis 2018-08-30 /pmc/articles/PMC6527380/ /pubmed/30160193 http://dx.doi.org/10.1080/19336918.2018.1506652 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Villanueva, Andrea A.
Puvogel, Sofía
Lois, Pablo
Muñoz-Palma, Ernesto
Ramírez Orellana, Manuel
Lubieniecki, Fabiana
Casco Claro, Fernando
Gallegos, Iván
García-Castro, Javier
Sanchez-Gomez, Pilar
Torres, Vicente A.
Palma, Verónica
The Netrin-4/Laminin γ1/Neogenin-1 complex mediates migration in SK-N-SH neuroblastoma cells
title The Netrin-4/Laminin γ1/Neogenin-1 complex mediates migration in SK-N-SH neuroblastoma cells
title_full The Netrin-4/Laminin γ1/Neogenin-1 complex mediates migration in SK-N-SH neuroblastoma cells
title_fullStr The Netrin-4/Laminin γ1/Neogenin-1 complex mediates migration in SK-N-SH neuroblastoma cells
title_full_unstemmed The Netrin-4/Laminin γ1/Neogenin-1 complex mediates migration in SK-N-SH neuroblastoma cells
title_short The Netrin-4/Laminin γ1/Neogenin-1 complex mediates migration in SK-N-SH neuroblastoma cells
title_sort netrin-4/laminin γ1/neogenin-1 complex mediates migration in sk-n-sh neuroblastoma cells
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527380/
https://www.ncbi.nlm.nih.gov/pubmed/30160193
http://dx.doi.org/10.1080/19336918.2018.1506652
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