Cargando…

Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells

The role of hyaluronan (HA) in periodontal healing has been speculated via its interaction with the CD44 receptor. While HA-CD44 interactions have previously been implicated in numerous cell types; effect and mechanism of exogenous HA on periodontal ligament (PDL) cells is less clear. Herein, we exa...

Descripción completa

Detalles Bibliográficos
Autores principales: Al-Rekabi, Zeinab, Fura, Adriane M., Juhlin, Ilsa, Yassin, Alaa, Popowics, Tracy E., Sniadecki, Nathan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527381/
https://www.ncbi.nlm.nih.gov/pubmed/30676222
http://dx.doi.org/10.1080/19336918.2019.1568140
_version_ 1783420025921601536
author Al-Rekabi, Zeinab
Fura, Adriane M.
Juhlin, Ilsa
Yassin, Alaa
Popowics, Tracy E.
Sniadecki, Nathan J.
author_facet Al-Rekabi, Zeinab
Fura, Adriane M.
Juhlin, Ilsa
Yassin, Alaa
Popowics, Tracy E.
Sniadecki, Nathan J.
author_sort Al-Rekabi, Zeinab
collection PubMed
description The role of hyaluronan (HA) in periodontal healing has been speculated via its interaction with the CD44 receptor. While HA-CD44 interactions have previously been implicated in numerous cell types; effect and mechanism of exogenous HA on periodontal ligament (PDL) cells is less clear. Herein, we examine the effect of exogenous HA on contractility and migration in human and murine PDL cells using arrays of microposts and time-lapse microscopy. Our findings observed HA-treated human PDL cells as more contractile and less migratory than untreated cells. Moreover, the effect of HA on contractility and focal adhesion area was abrogated when PDL cells were treated with Y27632, an inhibitor of rho-dependent kinase, but not when these cells were treated with ML-7, an inhibitor of myosin light chain kinase. Our results provide insight into the mechanobiology of PDL cells, which may contribute towards the development of therapeutic strategies for periodontal healing and tissue regeneration.
format Online
Article
Text
id pubmed-6527381
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-65273812019-05-31 Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells Al-Rekabi, Zeinab Fura, Adriane M. Juhlin, Ilsa Yassin, Alaa Popowics, Tracy E. Sniadecki, Nathan J. Cell Adh Migr Research Article The role of hyaluronan (HA) in periodontal healing has been speculated via its interaction with the CD44 receptor. While HA-CD44 interactions have previously been implicated in numerous cell types; effect and mechanism of exogenous HA on periodontal ligament (PDL) cells is less clear. Herein, we examine the effect of exogenous HA on contractility and migration in human and murine PDL cells using arrays of microposts and time-lapse microscopy. Our findings observed HA-treated human PDL cells as more contractile and less migratory than untreated cells. Moreover, the effect of HA on contractility and focal adhesion area was abrogated when PDL cells were treated with Y27632, an inhibitor of rho-dependent kinase, but not when these cells were treated with ML-7, an inhibitor of myosin light chain kinase. Our results provide insight into the mechanobiology of PDL cells, which may contribute towards the development of therapeutic strategies for periodontal healing and tissue regeneration. Taylor & Francis 2019-02-08 /pmc/articles/PMC6527381/ /pubmed/30676222 http://dx.doi.org/10.1080/19336918.2019.1568140 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Al-Rekabi, Zeinab
Fura, Adriane M.
Juhlin, Ilsa
Yassin, Alaa
Popowics, Tracy E.
Sniadecki, Nathan J.
Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells
title Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells
title_full Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells
title_fullStr Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells
title_full_unstemmed Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells
title_short Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells
title_sort hyaluronan-cd44 interactions mediate contractility and migration in periodontal ligament cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527381/
https://www.ncbi.nlm.nih.gov/pubmed/30676222
http://dx.doi.org/10.1080/19336918.2019.1568140
work_keys_str_mv AT alrekabizeinab hyaluronancd44interactionsmediatecontractilityandmigrationinperiodontalligamentcells
AT furaadrianem hyaluronancd44interactionsmediatecontractilityandmigrationinperiodontalligamentcells
AT juhlinilsa hyaluronancd44interactionsmediatecontractilityandmigrationinperiodontalligamentcells
AT yassinalaa hyaluronancd44interactionsmediatecontractilityandmigrationinperiodontalligamentcells
AT popowicstracye hyaluronancd44interactionsmediatecontractilityandmigrationinperiodontalligamentcells
AT sniadeckinathanj hyaluronancd44interactionsmediatecontractilityandmigrationinperiodontalligamentcells