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Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells
The role of hyaluronan (HA) in periodontal healing has been speculated via its interaction with the CD44 receptor. While HA-CD44 interactions have previously been implicated in numerous cell types; effect and mechanism of exogenous HA on periodontal ligament (PDL) cells is less clear. Herein, we exa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527381/ https://www.ncbi.nlm.nih.gov/pubmed/30676222 http://dx.doi.org/10.1080/19336918.2019.1568140 |
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author | Al-Rekabi, Zeinab Fura, Adriane M. Juhlin, Ilsa Yassin, Alaa Popowics, Tracy E. Sniadecki, Nathan J. |
author_facet | Al-Rekabi, Zeinab Fura, Adriane M. Juhlin, Ilsa Yassin, Alaa Popowics, Tracy E. Sniadecki, Nathan J. |
author_sort | Al-Rekabi, Zeinab |
collection | PubMed |
description | The role of hyaluronan (HA) in periodontal healing has been speculated via its interaction with the CD44 receptor. While HA-CD44 interactions have previously been implicated in numerous cell types; effect and mechanism of exogenous HA on periodontal ligament (PDL) cells is less clear. Herein, we examine the effect of exogenous HA on contractility and migration in human and murine PDL cells using arrays of microposts and time-lapse microscopy. Our findings observed HA-treated human PDL cells as more contractile and less migratory than untreated cells. Moreover, the effect of HA on contractility and focal adhesion area was abrogated when PDL cells were treated with Y27632, an inhibitor of rho-dependent kinase, but not when these cells were treated with ML-7, an inhibitor of myosin light chain kinase. Our results provide insight into the mechanobiology of PDL cells, which may contribute towards the development of therapeutic strategies for periodontal healing and tissue regeneration. |
format | Online Article Text |
id | pubmed-6527381 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-65273812019-05-31 Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells Al-Rekabi, Zeinab Fura, Adriane M. Juhlin, Ilsa Yassin, Alaa Popowics, Tracy E. Sniadecki, Nathan J. Cell Adh Migr Research Article The role of hyaluronan (HA) in periodontal healing has been speculated via its interaction with the CD44 receptor. While HA-CD44 interactions have previously been implicated in numerous cell types; effect and mechanism of exogenous HA on periodontal ligament (PDL) cells is less clear. Herein, we examine the effect of exogenous HA on contractility and migration in human and murine PDL cells using arrays of microposts and time-lapse microscopy. Our findings observed HA-treated human PDL cells as more contractile and less migratory than untreated cells. Moreover, the effect of HA on contractility and focal adhesion area was abrogated when PDL cells were treated with Y27632, an inhibitor of rho-dependent kinase, but not when these cells were treated with ML-7, an inhibitor of myosin light chain kinase. Our results provide insight into the mechanobiology of PDL cells, which may contribute towards the development of therapeutic strategies for periodontal healing and tissue regeneration. Taylor & Francis 2019-02-08 /pmc/articles/PMC6527381/ /pubmed/30676222 http://dx.doi.org/10.1080/19336918.2019.1568140 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Al-Rekabi, Zeinab Fura, Adriane M. Juhlin, Ilsa Yassin, Alaa Popowics, Tracy E. Sniadecki, Nathan J. Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells |
title | Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells |
title_full | Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells |
title_fullStr | Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells |
title_full_unstemmed | Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells |
title_short | Hyaluronan-CD44 interactions mediate contractility and migration in periodontal ligament cells |
title_sort | hyaluronan-cd44 interactions mediate contractility and migration in periodontal ligament cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527381/ https://www.ncbi.nlm.nih.gov/pubmed/30676222 http://dx.doi.org/10.1080/19336918.2019.1568140 |
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