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Multiplexed temporally focused light shaping through a gradient index lens for precise in-depth optogenetic photostimulation

In the past 10 years, the use of light has become irreplaceable for the optogenetic study and control of neurons and neural circuits. Optical techniques are however limited by scattering and can only see through a depth of few hundreds µm in living tissues. GRIN lens based micro-endoscopes represent...

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Autores principales: Accanto, Nicolò, Chen, I-Wen, Ronzitti, Emiliano, Molinier, Clément, Tourain, Christophe, Papagiakoumou, Eirini, Emiliani, Valentina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527563/
https://www.ncbi.nlm.nih.gov/pubmed/31110187
http://dx.doi.org/10.1038/s41598-019-43933-w
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author Accanto, Nicolò
Chen, I-Wen
Ronzitti, Emiliano
Molinier, Clément
Tourain, Christophe
Papagiakoumou, Eirini
Emiliani, Valentina
author_facet Accanto, Nicolò
Chen, I-Wen
Ronzitti, Emiliano
Molinier, Clément
Tourain, Christophe
Papagiakoumou, Eirini
Emiliani, Valentina
author_sort Accanto, Nicolò
collection PubMed
description In the past 10 years, the use of light has become irreplaceable for the optogenetic study and control of neurons and neural circuits. Optical techniques are however limited by scattering and can only see through a depth of few hundreds µm in living tissues. GRIN lens based micro-endoscopes represent a powerful solution to reach deeper regions. In this work we demonstrate that cutting edge optical methods for the precise photostimulation of multiple neurons in three dimensions can be performed through a GRIN lens. By spatio-temporally shaping a laser beam in the two-photon regime we project several tens of spatially confined targets in a volume of at least 100 × 150 × 300 µm(3). We then apply such approach to the optogenetic stimulation of multiple neurons simultaneously in vivo in mice. Our work paves the way for an all-optical investigation of neural circuits in previously inaccessible brain areas.
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spelling pubmed-65275632019-05-30 Multiplexed temporally focused light shaping through a gradient index lens for precise in-depth optogenetic photostimulation Accanto, Nicolò Chen, I-Wen Ronzitti, Emiliano Molinier, Clément Tourain, Christophe Papagiakoumou, Eirini Emiliani, Valentina Sci Rep Article In the past 10 years, the use of light has become irreplaceable for the optogenetic study and control of neurons and neural circuits. Optical techniques are however limited by scattering and can only see through a depth of few hundreds µm in living tissues. GRIN lens based micro-endoscopes represent a powerful solution to reach deeper regions. In this work we demonstrate that cutting edge optical methods for the precise photostimulation of multiple neurons in three dimensions can be performed through a GRIN lens. By spatio-temporally shaping a laser beam in the two-photon regime we project several tens of spatially confined targets in a volume of at least 100 × 150 × 300 µm(3). We then apply such approach to the optogenetic stimulation of multiple neurons simultaneously in vivo in mice. Our work paves the way for an all-optical investigation of neural circuits in previously inaccessible brain areas. Nature Publishing Group UK 2019-05-20 /pmc/articles/PMC6527563/ /pubmed/31110187 http://dx.doi.org/10.1038/s41598-019-43933-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Accanto, Nicolò
Chen, I-Wen
Ronzitti, Emiliano
Molinier, Clément
Tourain, Christophe
Papagiakoumou, Eirini
Emiliani, Valentina
Multiplexed temporally focused light shaping through a gradient index lens for precise in-depth optogenetic photostimulation
title Multiplexed temporally focused light shaping through a gradient index lens for precise in-depth optogenetic photostimulation
title_full Multiplexed temporally focused light shaping through a gradient index lens for precise in-depth optogenetic photostimulation
title_fullStr Multiplexed temporally focused light shaping through a gradient index lens for precise in-depth optogenetic photostimulation
title_full_unstemmed Multiplexed temporally focused light shaping through a gradient index lens for precise in-depth optogenetic photostimulation
title_short Multiplexed temporally focused light shaping through a gradient index lens for precise in-depth optogenetic photostimulation
title_sort multiplexed temporally focused light shaping through a gradient index lens for precise in-depth optogenetic photostimulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527563/
https://www.ncbi.nlm.nih.gov/pubmed/31110187
http://dx.doi.org/10.1038/s41598-019-43933-w
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