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Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis

Palmitic acid (PA) is the most common saturated long-chain fatty acid that causes damage to heart muscle cells. However, the molecular mechanism of PA toxicity in myocardial cells is not fully understood. In the present study, we explored the effects of PA on proliferation and apoptosis of H9c2 card...

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Autores principales: Yang, Lei, Guan, Gaopeng, Lei, Lanjie, Liu, Jianyun, Cao, Lingling, Wang, Xiangguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527925/
https://www.ncbi.nlm.nih.gov/pubmed/31064816
http://dx.doi.org/10.1042/BSR20190225
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author Yang, Lei
Guan, Gaopeng
Lei, Lanjie
Liu, Jianyun
Cao, Lingling
Wang, Xiangguo
author_facet Yang, Lei
Guan, Gaopeng
Lei, Lanjie
Liu, Jianyun
Cao, Lingling
Wang, Xiangguo
author_sort Yang, Lei
collection PubMed
description Palmitic acid (PA) is the most common saturated long-chain fatty acid that causes damage to heart muscle cells. However, the molecular mechanism of PA toxicity in myocardial cells is not fully understood. In the present study, we explored the effects of PA on proliferation and apoptosis of H9c2 cardiomyocytes, and uncovered the signaling pathways involved in PA toxicity. Our study revealed induction of both oxidative and endoplasmic reticulum (ER) stresses and exacerbation of apoptosis in PA-treated H9c2 cells. Inhibition of oxidative stress by N-acetylcysteine (NAC) reduced apoptosis and decreased ER stress in PA-treated H9c2 cells. Moreover, inhibition of ER stress by 4-phenyl butyric acid decreased apoptosis and attenuated oxidative stress. In summary, the present study demonstrated that oxidative stress coordinates with ER stress to play important roles in PA-induced H9c2 cell apoptosis.
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spelling pubmed-65279252019-05-30 Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis Yang, Lei Guan, Gaopeng Lei, Lanjie Liu, Jianyun Cao, Lingling Wang, Xiangguo Biosci Rep Research Articles Palmitic acid (PA) is the most common saturated long-chain fatty acid that causes damage to heart muscle cells. However, the molecular mechanism of PA toxicity in myocardial cells is not fully understood. In the present study, we explored the effects of PA on proliferation and apoptosis of H9c2 cardiomyocytes, and uncovered the signaling pathways involved in PA toxicity. Our study revealed induction of both oxidative and endoplasmic reticulum (ER) stresses and exacerbation of apoptosis in PA-treated H9c2 cells. Inhibition of oxidative stress by N-acetylcysteine (NAC) reduced apoptosis and decreased ER stress in PA-treated H9c2 cells. Moreover, inhibition of ER stress by 4-phenyl butyric acid decreased apoptosis and attenuated oxidative stress. In summary, the present study demonstrated that oxidative stress coordinates with ER stress to play important roles in PA-induced H9c2 cell apoptosis. Portland Press Ltd. 2019-05-21 /pmc/articles/PMC6527925/ /pubmed/31064816 http://dx.doi.org/10.1042/BSR20190225 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Yang, Lei
Guan, Gaopeng
Lei, Lanjie
Liu, Jianyun
Cao, Lingling
Wang, Xiangguo
Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis
title Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis
title_full Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis
title_fullStr Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis
title_full_unstemmed Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis
title_short Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis
title_sort oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced h9c2 cell apoptosis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527925/
https://www.ncbi.nlm.nih.gov/pubmed/31064816
http://dx.doi.org/10.1042/BSR20190225
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