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Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis
Palmitic acid (PA) is the most common saturated long-chain fatty acid that causes damage to heart muscle cells. However, the molecular mechanism of PA toxicity in myocardial cells is not fully understood. In the present study, we explored the effects of PA on proliferation and apoptosis of H9c2 card...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527925/ https://www.ncbi.nlm.nih.gov/pubmed/31064816 http://dx.doi.org/10.1042/BSR20190225 |
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author | Yang, Lei Guan, Gaopeng Lei, Lanjie Liu, Jianyun Cao, Lingling Wang, Xiangguo |
author_facet | Yang, Lei Guan, Gaopeng Lei, Lanjie Liu, Jianyun Cao, Lingling Wang, Xiangguo |
author_sort | Yang, Lei |
collection | PubMed |
description | Palmitic acid (PA) is the most common saturated long-chain fatty acid that causes damage to heart muscle cells. However, the molecular mechanism of PA toxicity in myocardial cells is not fully understood. In the present study, we explored the effects of PA on proliferation and apoptosis of H9c2 cardiomyocytes, and uncovered the signaling pathways involved in PA toxicity. Our study revealed induction of both oxidative and endoplasmic reticulum (ER) stresses and exacerbation of apoptosis in PA-treated H9c2 cells. Inhibition of oxidative stress by N-acetylcysteine (NAC) reduced apoptosis and decreased ER stress in PA-treated H9c2 cells. Moreover, inhibition of ER stress by 4-phenyl butyric acid decreased apoptosis and attenuated oxidative stress. In summary, the present study demonstrated that oxidative stress coordinates with ER stress to play important roles in PA-induced H9c2 cell apoptosis. |
format | Online Article Text |
id | pubmed-6527925 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65279252019-05-30 Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis Yang, Lei Guan, Gaopeng Lei, Lanjie Liu, Jianyun Cao, Lingling Wang, Xiangguo Biosci Rep Research Articles Palmitic acid (PA) is the most common saturated long-chain fatty acid that causes damage to heart muscle cells. However, the molecular mechanism of PA toxicity in myocardial cells is not fully understood. In the present study, we explored the effects of PA on proliferation and apoptosis of H9c2 cardiomyocytes, and uncovered the signaling pathways involved in PA toxicity. Our study revealed induction of both oxidative and endoplasmic reticulum (ER) stresses and exacerbation of apoptosis in PA-treated H9c2 cells. Inhibition of oxidative stress by N-acetylcysteine (NAC) reduced apoptosis and decreased ER stress in PA-treated H9c2 cells. Moreover, inhibition of ER stress by 4-phenyl butyric acid decreased apoptosis and attenuated oxidative stress. In summary, the present study demonstrated that oxidative stress coordinates with ER stress to play important roles in PA-induced H9c2 cell apoptosis. Portland Press Ltd. 2019-05-21 /pmc/articles/PMC6527925/ /pubmed/31064816 http://dx.doi.org/10.1042/BSR20190225 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Yang, Lei Guan, Gaopeng Lei, Lanjie Liu, Jianyun Cao, Lingling Wang, Xiangguo Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis |
title | Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis |
title_full | Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis |
title_fullStr | Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis |
title_full_unstemmed | Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis |
title_short | Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis |
title_sort | oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced h9c2 cell apoptosis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6527925/ https://www.ncbi.nlm.nih.gov/pubmed/31064816 http://dx.doi.org/10.1042/BSR20190225 |
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