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Oxidative stress mediates depot-specific functional differences of human adipose-derived stem cells

BACKGROUND: Visceral (VS) fat depot is known to have defective adipogenic functions compared to subcutaneous (SC) fat, but its mechanism of origin is unclear. OBJECTIVE: We tested our hypothesis that the degree of oxidative stress in adipose-derived stem cells (ASCs) from these depots may account fo...

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Autores principales: Sriram, Sandhya, Yuan, Chengxiang, Chakraborty, Smarajit, Tay, Winson, Park, Min, Shabbir, Asim, Toh, Sue-Anne, Han, Weiping, Sugii, Shigeki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6528291/
https://www.ncbi.nlm.nih.gov/pubmed/31113471
http://dx.doi.org/10.1186/s13287-019-1240-y
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author Sriram, Sandhya
Yuan, Chengxiang
Chakraborty, Smarajit
Tay, Winson
Park, Min
Shabbir, Asim
Toh, Sue-Anne
Han, Weiping
Sugii, Shigeki
author_facet Sriram, Sandhya
Yuan, Chengxiang
Chakraborty, Smarajit
Tay, Winson
Park, Min
Shabbir, Asim
Toh, Sue-Anne
Han, Weiping
Sugii, Shigeki
author_sort Sriram, Sandhya
collection PubMed
description BACKGROUND: Visceral (VS) fat depot is known to have defective adipogenic functions compared to subcutaneous (SC) fat, but its mechanism of origin is unclear. OBJECTIVE: We tested our hypothesis that the degree of oxidative stress in adipose-derived stem cells (ASCs) from these depots may account for this difference. METHODS: ASCs were isolated from VS (omental region) and SC (abdominal region) fat depots of human subjects undergoing bariatric surgery. ASCs from VS and SC fat were investigated for their cellular characteristics in reactive oxygen species (ROS), metabolism, gene expression, proliferation, senescence, migration, and adipocyte differentiation. ASCs were also treated with antioxidant ascorbic acid (vitamin C). RESULTS: We found that human VS-derived ASCs exhibit excessive oxidative stress characterized by high reactive oxygen species (ROS), compared to SC-derived ASCs. Gene expression analyses indicate that the VS-ASCs exhibit higher levels of genes involved in pro-oxidant and pro-inflammatory pathways and lower levels of genes in antioxidant and anti-inflammatory pathways. VS-ASCs have impaired cellular functions compared to SC-ASCs, such as slower proliferation, early senescence, less migratory activity, and poor adipogenic capability in vitro. Treatment with ascorbic acid decreased ROS levels drastically in VS-ASCs. Ascorbic acid treatment substantially improved proliferation, senescence, migration, and adipogenic capacities of compromised ASCs caused by high ROS. CONCLUSIONS: This finding suggests the fat depot-specific differences of cellular defects originating from stem cell population. Considering clinical potentials of human ASCs for cell therapies, this also offers a possible strategy for improving their therapeutic qualities through antioxidants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13287-019-1240-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-65282912019-05-28 Oxidative stress mediates depot-specific functional differences of human adipose-derived stem cells Sriram, Sandhya Yuan, Chengxiang Chakraborty, Smarajit Tay, Winson Park, Min Shabbir, Asim Toh, Sue-Anne Han, Weiping Sugii, Shigeki Stem Cell Res Ther Short Report BACKGROUND: Visceral (VS) fat depot is known to have defective adipogenic functions compared to subcutaneous (SC) fat, but its mechanism of origin is unclear. OBJECTIVE: We tested our hypothesis that the degree of oxidative stress in adipose-derived stem cells (ASCs) from these depots may account for this difference. METHODS: ASCs were isolated from VS (omental region) and SC (abdominal region) fat depots of human subjects undergoing bariatric surgery. ASCs from VS and SC fat were investigated for their cellular characteristics in reactive oxygen species (ROS), metabolism, gene expression, proliferation, senescence, migration, and adipocyte differentiation. ASCs were also treated with antioxidant ascorbic acid (vitamin C). RESULTS: We found that human VS-derived ASCs exhibit excessive oxidative stress characterized by high reactive oxygen species (ROS), compared to SC-derived ASCs. Gene expression analyses indicate that the VS-ASCs exhibit higher levels of genes involved in pro-oxidant and pro-inflammatory pathways and lower levels of genes in antioxidant and anti-inflammatory pathways. VS-ASCs have impaired cellular functions compared to SC-ASCs, such as slower proliferation, early senescence, less migratory activity, and poor adipogenic capability in vitro. Treatment with ascorbic acid decreased ROS levels drastically in VS-ASCs. Ascorbic acid treatment substantially improved proliferation, senescence, migration, and adipogenic capacities of compromised ASCs caused by high ROS. CONCLUSIONS: This finding suggests the fat depot-specific differences of cellular defects originating from stem cell population. Considering clinical potentials of human ASCs for cell therapies, this also offers a possible strategy for improving their therapeutic qualities through antioxidants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13287-019-1240-y) contains supplementary material, which is available to authorized users. BioMed Central 2019-05-21 /pmc/articles/PMC6528291/ /pubmed/31113471 http://dx.doi.org/10.1186/s13287-019-1240-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Sriram, Sandhya
Yuan, Chengxiang
Chakraborty, Smarajit
Tay, Winson
Park, Min
Shabbir, Asim
Toh, Sue-Anne
Han, Weiping
Sugii, Shigeki
Oxidative stress mediates depot-specific functional differences of human adipose-derived stem cells
title Oxidative stress mediates depot-specific functional differences of human adipose-derived stem cells
title_full Oxidative stress mediates depot-specific functional differences of human adipose-derived stem cells
title_fullStr Oxidative stress mediates depot-specific functional differences of human adipose-derived stem cells
title_full_unstemmed Oxidative stress mediates depot-specific functional differences of human adipose-derived stem cells
title_short Oxidative stress mediates depot-specific functional differences of human adipose-derived stem cells
title_sort oxidative stress mediates depot-specific functional differences of human adipose-derived stem cells
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6528291/
https://www.ncbi.nlm.nih.gov/pubmed/31113471
http://dx.doi.org/10.1186/s13287-019-1240-y
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