Cargando…
Three-dimensional label-free imaging throughout adipocyte differentiation by stimulated Raman microscopy
Lipid droplets are lipid-storage organelles with a key role in lipid accumulation pathologies such as diabetes, obesity and atherosclerosis. Despite their important functions many aspects of lipid droplets biology are still unknown. This is partially due to the current use of exogenous labels to mon...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6528968/ https://www.ncbi.nlm.nih.gov/pubmed/31112567 http://dx.doi.org/10.1371/journal.pone.0216811 |
_version_ | 1783420309397831680 |
---|---|
author | Ferrara, Maria Antonietta Filograna, Angela Ranjan, Rajeev Corda, Daniela Valente, Carmen Sirleto, Luigi |
author_facet | Ferrara, Maria Antonietta Filograna, Angela Ranjan, Rajeev Corda, Daniela Valente, Carmen Sirleto, Luigi |
author_sort | Ferrara, Maria Antonietta |
collection | PubMed |
description | Lipid droplets are lipid-storage organelles with a key role in lipid accumulation pathologies such as diabetes, obesity and atherosclerosis. Despite their important functions many aspects of lipid droplets biology are still unknown. This is partially due to the current use of exogenous labels to monitor their formation and remodelling by invasive imaging methods. Here, we apply stimulated Raman scattering microscopy to acquire images with high spatial resolution along with resolving capabilities of lipids and proteins and three-dimensional sectioning. Our images and data analysis demonstrate an increase in the number of large (>15μm(2)) lipid droplets in human adipocyte cells during differentiation process. In addition, spatially-resolved maps of lipids and proteins inside cells and three dimensional reconstructions of lipids at the initial and final steps of adipocyte differentiation are reported, too. |
format | Online Article Text |
id | pubmed-6528968 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-65289682019-05-31 Three-dimensional label-free imaging throughout adipocyte differentiation by stimulated Raman microscopy Ferrara, Maria Antonietta Filograna, Angela Ranjan, Rajeev Corda, Daniela Valente, Carmen Sirleto, Luigi PLoS One Research Article Lipid droplets are lipid-storage organelles with a key role in lipid accumulation pathologies such as diabetes, obesity and atherosclerosis. Despite their important functions many aspects of lipid droplets biology are still unknown. This is partially due to the current use of exogenous labels to monitor their formation and remodelling by invasive imaging methods. Here, we apply stimulated Raman scattering microscopy to acquire images with high spatial resolution along with resolving capabilities of lipids and proteins and three-dimensional sectioning. Our images and data analysis demonstrate an increase in the number of large (>15μm(2)) lipid droplets in human adipocyte cells during differentiation process. In addition, spatially-resolved maps of lipids and proteins inside cells and three dimensional reconstructions of lipids at the initial and final steps of adipocyte differentiation are reported, too. Public Library of Science 2019-05-21 /pmc/articles/PMC6528968/ /pubmed/31112567 http://dx.doi.org/10.1371/journal.pone.0216811 Text en © 2019 Ferrara et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ferrara, Maria Antonietta Filograna, Angela Ranjan, Rajeev Corda, Daniela Valente, Carmen Sirleto, Luigi Three-dimensional label-free imaging throughout adipocyte differentiation by stimulated Raman microscopy |
title | Three-dimensional label-free imaging throughout adipocyte differentiation by stimulated Raman microscopy |
title_full | Three-dimensional label-free imaging throughout adipocyte differentiation by stimulated Raman microscopy |
title_fullStr | Three-dimensional label-free imaging throughout adipocyte differentiation by stimulated Raman microscopy |
title_full_unstemmed | Three-dimensional label-free imaging throughout adipocyte differentiation by stimulated Raman microscopy |
title_short | Three-dimensional label-free imaging throughout adipocyte differentiation by stimulated Raman microscopy |
title_sort | three-dimensional label-free imaging throughout adipocyte differentiation by stimulated raman microscopy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6528968/ https://www.ncbi.nlm.nih.gov/pubmed/31112567 http://dx.doi.org/10.1371/journal.pone.0216811 |
work_keys_str_mv | AT ferraramariaantonietta threedimensionallabelfreeimagingthroughoutadipocytedifferentiationbystimulatedramanmicroscopy AT filogranaangela threedimensionallabelfreeimagingthroughoutadipocytedifferentiationbystimulatedramanmicroscopy AT ranjanrajeev threedimensionallabelfreeimagingthroughoutadipocytedifferentiationbystimulatedramanmicroscopy AT cordadaniela threedimensionallabelfreeimagingthroughoutadipocytedifferentiationbystimulatedramanmicroscopy AT valentecarmen threedimensionallabelfreeimagingthroughoutadipocytedifferentiationbystimulatedramanmicroscopy AT sirletoluigi threedimensionallabelfreeimagingthroughoutadipocytedifferentiationbystimulatedramanmicroscopy |