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Prevalence of human pathogens of the clade Nakaseomyces in a culture collection—the first report on Candida bracarensis in Poland
Human pathogens belonging to the Nakaseomyces clade include Candida glabrata sensu stricto, Candida nivariensis and Candida bracarensis. Their highly similar phenotypic characteristics often lead to misidentification by conventional laboratory methods. Therefore, limited information on the true epid...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529382/ https://www.ncbi.nlm.nih.gov/pubmed/30361876 http://dx.doi.org/10.1007/s12223-018-0655-7 |
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author | Małek, Marianna Mrowiec, Paulina Klesiewicz, Karolina Skiba-Kurek, Iwona Szczepański, Adrian Białecka, Joanna Żak, Iwona Bogusz, Bożena Kędzierska, Jolanta Budak, Alicja Karczewska, Elżbieta |
author_facet | Małek, Marianna Mrowiec, Paulina Klesiewicz, Karolina Skiba-Kurek, Iwona Szczepański, Adrian Białecka, Joanna Żak, Iwona Bogusz, Bożena Kędzierska, Jolanta Budak, Alicja Karczewska, Elżbieta |
author_sort | Małek, Marianna |
collection | PubMed |
description | Human pathogens belonging to the Nakaseomyces clade include Candida glabrata sensu stricto, Candida nivariensis and Candida bracarensis. Their highly similar phenotypic characteristics often lead to misidentification by conventional laboratory methods. Therefore, limited information on the true epidemiology of the Candida glabrata species complex is available. Due to life-threatening infections caused by these species, it is crucial to supplement this knowledge. The aim of the study was to estimate the prevalence of C. bracarensis and C. nivariensis in a culture collection of C. glabrata complex isolates. The study covered 353 isolates identified by biochemical methods as C. glabrata, collected from paediatric and adult patients hospitalised at four medical centres in Southern Poland. The multiplex PCR was used to identify the strains. Further species confirmation was performed via sequencing and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) analysis. One isolate was recognised as C. bracarensis (0.28%). To our knowledge, it is the first isolate in Poland. C. glabrata sensu stricto species has been confirmed for all the remaining isolates. No C. nivariensis was found. Our study has shown that the prevalence of C. nivariensis and C. bracarensis strains is infrequent. However, it should be emphasised that the incidence of these strains may differ locally and depend on environmental factors and the population. |
format | Online Article Text |
id | pubmed-6529382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-65293822019-06-07 Prevalence of human pathogens of the clade Nakaseomyces in a culture collection—the first report on Candida bracarensis in Poland Małek, Marianna Mrowiec, Paulina Klesiewicz, Karolina Skiba-Kurek, Iwona Szczepański, Adrian Białecka, Joanna Żak, Iwona Bogusz, Bożena Kędzierska, Jolanta Budak, Alicja Karczewska, Elżbieta Folia Microbiol (Praha) Original Article Human pathogens belonging to the Nakaseomyces clade include Candida glabrata sensu stricto, Candida nivariensis and Candida bracarensis. Their highly similar phenotypic characteristics often lead to misidentification by conventional laboratory methods. Therefore, limited information on the true epidemiology of the Candida glabrata species complex is available. Due to life-threatening infections caused by these species, it is crucial to supplement this knowledge. The aim of the study was to estimate the prevalence of C. bracarensis and C. nivariensis in a culture collection of C. glabrata complex isolates. The study covered 353 isolates identified by biochemical methods as C. glabrata, collected from paediatric and adult patients hospitalised at four medical centres in Southern Poland. The multiplex PCR was used to identify the strains. Further species confirmation was performed via sequencing and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) analysis. One isolate was recognised as C. bracarensis (0.28%). To our knowledge, it is the first isolate in Poland. C. glabrata sensu stricto species has been confirmed for all the remaining isolates. No C. nivariensis was found. Our study has shown that the prevalence of C. nivariensis and C. bracarensis strains is infrequent. However, it should be emphasised that the incidence of these strains may differ locally and depend on environmental factors and the population. Springer Netherlands 2018-10-25 2019 /pmc/articles/PMC6529382/ /pubmed/30361876 http://dx.doi.org/10.1007/s12223-018-0655-7 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Małek, Marianna Mrowiec, Paulina Klesiewicz, Karolina Skiba-Kurek, Iwona Szczepański, Adrian Białecka, Joanna Żak, Iwona Bogusz, Bożena Kędzierska, Jolanta Budak, Alicja Karczewska, Elżbieta Prevalence of human pathogens of the clade Nakaseomyces in a culture collection—the first report on Candida bracarensis in Poland |
title | Prevalence of human pathogens of the clade Nakaseomyces in a culture collection—the first report on Candida bracarensis in Poland |
title_full | Prevalence of human pathogens of the clade Nakaseomyces in a culture collection—the first report on Candida bracarensis in Poland |
title_fullStr | Prevalence of human pathogens of the clade Nakaseomyces in a culture collection—the first report on Candida bracarensis in Poland |
title_full_unstemmed | Prevalence of human pathogens of the clade Nakaseomyces in a culture collection—the first report on Candida bracarensis in Poland |
title_short | Prevalence of human pathogens of the clade Nakaseomyces in a culture collection—the first report on Candida bracarensis in Poland |
title_sort | prevalence of human pathogens of the clade nakaseomyces in a culture collection—the first report on candida bracarensis in poland |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529382/ https://www.ncbi.nlm.nih.gov/pubmed/30361876 http://dx.doi.org/10.1007/s12223-018-0655-7 |
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