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Molecular Organization and Chromosomal Localization Analysis of 5S rDNA Clusters in Autotetraploids Derived From Carassius auratus Red Var. (♀) × Megalobrama amblycephala (♂)
The autotetraploid fish (4n = 200, RRRR) (abbreviated as 4nRR) resulted from the whole genome duplication of red crucian carp (Carassius auratus red var., 2n = 100, RR) (abbreviated as RCC). During investigation of the influence of polyploidization on organization and evolution of the multigene fami...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529582/ https://www.ncbi.nlm.nih.gov/pubmed/31156700 http://dx.doi.org/10.3389/fgene.2019.00437 |
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author | Qin, QinBo Liu, QiWen Wang, ChongQing Cao, Liu Zhou, YuWei Qin, Huan Zhao, Chun Liu, ShaoJun |
author_facet | Qin, QinBo Liu, QiWen Wang, ChongQing Cao, Liu Zhou, YuWei Qin, Huan Zhao, Chun Liu, ShaoJun |
author_sort | Qin, QinBo |
collection | PubMed |
description | The autotetraploid fish (4n = 200, RRRR) (abbreviated as 4nRR) resulted from the whole genome duplication of red crucian carp (Carassius auratus red var., 2n = 100, RR) (abbreviated as RCC). During investigation of the influence of polyploidization on organization and evolution of the multigene family of 5S rDNA, molecular organization and chromosomal localization of the 5S rDNA were characterized in autotetraploid fish. By sequence analysis of the coding region (5S) and adjacent non-transcribed spacer (NTS), three distinct 5S rDNA units (type I: 203 bp; type II: 340 bp; and type III: 477bp) were identified and characterized in 4nRR. These 5S rDNA units were inherited from their female parent (RCC), in which obvious base variations in NTS and array recombination of repeat units were found. Using fluorescence in situ hybridization employing different 5S rDNA units as probes, these 5S rDNA clusters were localized in chromosomes of 4nRR, respectively, and showed obvious loss of chromosomal loci (type I and type II). Our data revealed genetic variation of the 5S rDNA multigene family in the genome of autopolyploid fish. Furthermore, results provided new insights into the evolutionary patterns of this vertebrate multigene family. |
format | Online Article Text |
id | pubmed-6529582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-65295822019-05-31 Molecular Organization and Chromosomal Localization Analysis of 5S rDNA Clusters in Autotetraploids Derived From Carassius auratus Red Var. (♀) × Megalobrama amblycephala (♂) Qin, QinBo Liu, QiWen Wang, ChongQing Cao, Liu Zhou, YuWei Qin, Huan Zhao, Chun Liu, ShaoJun Front Genet Genetics The autotetraploid fish (4n = 200, RRRR) (abbreviated as 4nRR) resulted from the whole genome duplication of red crucian carp (Carassius auratus red var., 2n = 100, RR) (abbreviated as RCC). During investigation of the influence of polyploidization on organization and evolution of the multigene family of 5S rDNA, molecular organization and chromosomal localization of the 5S rDNA were characterized in autotetraploid fish. By sequence analysis of the coding region (5S) and adjacent non-transcribed spacer (NTS), three distinct 5S rDNA units (type I: 203 bp; type II: 340 bp; and type III: 477bp) were identified and characterized in 4nRR. These 5S rDNA units were inherited from their female parent (RCC), in which obvious base variations in NTS and array recombination of repeat units were found. Using fluorescence in situ hybridization employing different 5S rDNA units as probes, these 5S rDNA clusters were localized in chromosomes of 4nRR, respectively, and showed obvious loss of chromosomal loci (type I and type II). Our data revealed genetic variation of the 5S rDNA multigene family in the genome of autopolyploid fish. Furthermore, results provided new insights into the evolutionary patterns of this vertebrate multigene family. Frontiers Media S.A. 2019-05-15 /pmc/articles/PMC6529582/ /pubmed/31156700 http://dx.doi.org/10.3389/fgene.2019.00437 Text en Copyright © 2019 Qin, Liu, Wang, Cao, Zhou, Qin, Zhao and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Qin, QinBo Liu, QiWen Wang, ChongQing Cao, Liu Zhou, YuWei Qin, Huan Zhao, Chun Liu, ShaoJun Molecular Organization and Chromosomal Localization Analysis of 5S rDNA Clusters in Autotetraploids Derived From Carassius auratus Red Var. (♀) × Megalobrama amblycephala (♂) |
title | Molecular Organization and Chromosomal Localization Analysis of 5S rDNA Clusters in Autotetraploids Derived From Carassius auratus Red Var. (♀) × Megalobrama amblycephala (♂) |
title_full | Molecular Organization and Chromosomal Localization Analysis of 5S rDNA Clusters in Autotetraploids Derived From Carassius auratus Red Var. (♀) × Megalobrama amblycephala (♂) |
title_fullStr | Molecular Organization and Chromosomal Localization Analysis of 5S rDNA Clusters in Autotetraploids Derived From Carassius auratus Red Var. (♀) × Megalobrama amblycephala (♂) |
title_full_unstemmed | Molecular Organization and Chromosomal Localization Analysis of 5S rDNA Clusters in Autotetraploids Derived From Carassius auratus Red Var. (♀) × Megalobrama amblycephala (♂) |
title_short | Molecular Organization and Chromosomal Localization Analysis of 5S rDNA Clusters in Autotetraploids Derived From Carassius auratus Red Var. (♀) × Megalobrama amblycephala (♂) |
title_sort | molecular organization and chromosomal localization analysis of 5s rdna clusters in autotetraploids derived from carassius auratus red var. (♀) × megalobrama amblycephala (♂) |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529582/ https://www.ncbi.nlm.nih.gov/pubmed/31156700 http://dx.doi.org/10.3389/fgene.2019.00437 |
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