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Bacterial biofilm in salivary stones

PURPOSE: To assess the susceptibility of salivary stones to bacterial biofilm formation, which may be involved in the development of salivary gland infection, and to investigate a relation between microbiological aspects and patient characteristics. METHODS: This prospective study comprises of 54 pa...

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Autores principales: Perez-Tanoira, Ramón, Aarnisalo, Antti, Haapaniemi, Aaro, Saarinen, Riitta, Kuusela, Pentti, Kinnari, Teemu J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529597/
https://www.ncbi.nlm.nih.gov/pubmed/31028534
http://dx.doi.org/10.1007/s00405-019-05445-1
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author Perez-Tanoira, Ramón
Aarnisalo, Antti
Haapaniemi, Aaro
Saarinen, Riitta
Kuusela, Pentti
Kinnari, Teemu J.
author_facet Perez-Tanoira, Ramón
Aarnisalo, Antti
Haapaniemi, Aaro
Saarinen, Riitta
Kuusela, Pentti
Kinnari, Teemu J.
author_sort Perez-Tanoira, Ramón
collection PubMed
description PURPOSE: To assess the susceptibility of salivary stones to bacterial biofilm formation, which may be involved in the development of salivary gland infection, and to investigate a relation between microbiological aspects and patient characteristics. METHODS: This prospective study comprises of 54 patients with sialolithiasis attended in Helsinki University Hospital during 2014–2016. A total of 55 salivary stones were removed, and studied for biofilm formation using fluorescence microscopy and sonication. The isolated organisms were quantified and identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. RESULTS: Biofilm formation was confirmed on the surface of 39 (70.9%) stones. A total of 96 microorganisms were isolated from 45 salivary stones (81.8%). Two or more organisms were isolated in 33 (73.3%) cases. The main isolates were Streptococcus mitis/oralis (n = 27; 28.1%), followed by Streptococcus anginosus (n = 10; 9.6%), Rothia spp. (n = 8; 8.3%), Streptococcus constellatus (n = 7; 7.3%), and Streptococcus gordonii (n = 6; 6.2%). In all patients showing pre-operative (12 cases) or peri-operative (three cases) drainage of pus, the presence of biofilm was detected in microscopy (p = 0.004). Four patients showed post-operative infection, and in three of them (75.0%), the presence of biofilm was detected. Increased number of pus drainage was found among patients with reflux symptoms or use of proton-pump inhibitors. CONCLUSIONS: Salivary stones are susceptible to bacterial biofilm formation, which could be related with the development and severity of the inflammation and the refractory nature of the disease. Sonication of salivary gland stones could be a useful method for finding the etiology of the chronic infection.
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spelling pubmed-65295972019-06-07 Bacterial biofilm in salivary stones Perez-Tanoira, Ramón Aarnisalo, Antti Haapaniemi, Aaro Saarinen, Riitta Kuusela, Pentti Kinnari, Teemu J. Eur Arch Otorhinolaryngol Head & Neck PURPOSE: To assess the susceptibility of salivary stones to bacterial biofilm formation, which may be involved in the development of salivary gland infection, and to investigate a relation between microbiological aspects and patient characteristics. METHODS: This prospective study comprises of 54 patients with sialolithiasis attended in Helsinki University Hospital during 2014–2016. A total of 55 salivary stones were removed, and studied for biofilm formation using fluorescence microscopy and sonication. The isolated organisms were quantified and identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. RESULTS: Biofilm formation was confirmed on the surface of 39 (70.9%) stones. A total of 96 microorganisms were isolated from 45 salivary stones (81.8%). Two or more organisms were isolated in 33 (73.3%) cases. The main isolates were Streptococcus mitis/oralis (n = 27; 28.1%), followed by Streptococcus anginosus (n = 10; 9.6%), Rothia spp. (n = 8; 8.3%), Streptococcus constellatus (n = 7; 7.3%), and Streptococcus gordonii (n = 6; 6.2%). In all patients showing pre-operative (12 cases) or peri-operative (three cases) drainage of pus, the presence of biofilm was detected in microscopy (p = 0.004). Four patients showed post-operative infection, and in three of them (75.0%), the presence of biofilm was detected. Increased number of pus drainage was found among patients with reflux symptoms or use of proton-pump inhibitors. CONCLUSIONS: Salivary stones are susceptible to bacterial biofilm formation, which could be related with the development and severity of the inflammation and the refractory nature of the disease. Sonication of salivary gland stones could be a useful method for finding the etiology of the chronic infection. Springer Berlin Heidelberg 2019-04-26 2019 /pmc/articles/PMC6529597/ /pubmed/31028534 http://dx.doi.org/10.1007/s00405-019-05445-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Head & Neck
Perez-Tanoira, Ramón
Aarnisalo, Antti
Haapaniemi, Aaro
Saarinen, Riitta
Kuusela, Pentti
Kinnari, Teemu J.
Bacterial biofilm in salivary stones
title Bacterial biofilm in salivary stones
title_full Bacterial biofilm in salivary stones
title_fullStr Bacterial biofilm in salivary stones
title_full_unstemmed Bacterial biofilm in salivary stones
title_short Bacterial biofilm in salivary stones
title_sort bacterial biofilm in salivary stones
topic Head & Neck
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529597/
https://www.ncbi.nlm.nih.gov/pubmed/31028534
http://dx.doi.org/10.1007/s00405-019-05445-1
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