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In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression

BACKGROUND: Identification and assessment of therapeutic potential of natural products derived from medicinal plants have led to the discovery of innovative and economical drugs to treat several diseases, including chronic wounds. In vitro cell based scratch assay is an appropriate and inexpensive m...

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Autores principales: Bolla, Srinivasa Rao, Mohammed Al-Subaie, Abeer, Yousuf Al-Jindan, Reem, Papayya Balakrishna, Janardhana, Kanchi Ravi, Padma, Veeraraghavan, Vishnu Priya, Arumugam Pillai, Aruthra, Gollapalli, Shiva Shankar Reddy, Palpath Joseph, Joel, Surapaneni, Krishna Mohan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529694/
https://www.ncbi.nlm.nih.gov/pubmed/31193473
http://dx.doi.org/10.1016/j.heliyon.2019.e01648
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author Bolla, Srinivasa Rao
Mohammed Al-Subaie, Abeer
Yousuf Al-Jindan, Reem
Papayya Balakrishna, Janardhana
Kanchi Ravi, Padma
Veeraraghavan, Vishnu Priya
Arumugam Pillai, Aruthra
Gollapalli, Shiva Shankar Reddy
Palpath Joseph, Joel
Surapaneni, Krishna Mohan
author_facet Bolla, Srinivasa Rao
Mohammed Al-Subaie, Abeer
Yousuf Al-Jindan, Reem
Papayya Balakrishna, Janardhana
Kanchi Ravi, Padma
Veeraraghavan, Vishnu Priya
Arumugam Pillai, Aruthra
Gollapalli, Shiva Shankar Reddy
Palpath Joseph, Joel
Surapaneni, Krishna Mohan
author_sort Bolla, Srinivasa Rao
collection PubMed
description BACKGROUND: Identification and assessment of therapeutic potential of natural products derived from medicinal plants have led to the discovery of innovative and economical drugs to treat several diseases, including chronic wounds. In vitro cell based scratch assay is an appropriate and inexpensive method for initial understanding of wound healing potential of medicinal plant extracts. The current study was aimed at investigating the wound healing capacity of Aristolochia saccata leaf extract by using scratch assay as a primary model, where proliferative and migratory capabilities of test compounds could be monitored through microscopy studies. A. saccata is an evergreen climbing shrub belongs to the family Aristolochiaceae. METHODS: Methanolic extraction of the plant material was done using Soxhlet apparatus and the cytotoxicity of the extract on L929 cells was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. L929 is a human fibroblast cell line. In vitro scratch assay was performed to evaluate the wound healing properties of A. saccata leaf extract and possible mechanism of action was analyzed by flow cytometric expression studies of an extracellular matrix (ECM) factor, collagen type-1. RESULTS: MTT assay revealed that A. saccata leaf extract had no cytotoxic effect on the cells and at higher concentrations, the extract showed mild toxicity resulting in the death of just 2.88% cells. Scratch assay showed 34.05%, 70.00%, 93.52% wound closure at 12hrs, 24hrs and 48hrs of incubation respectively. These results were similar compared to positive control which showed 37.60, 56.41 and 99.05% of wound closure. Further, flow cytometry-based studies revealed that the A. saccata leaf extract induced the expression of ECM remodelling factor collagen-1. CONCLUSION: Our study revealed the wound healing capabilities of A. saccata In vitro. Hence, A. saccata could be recommended as a potential source of wound healing agents.
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spelling pubmed-65296942019-05-28 In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression Bolla, Srinivasa Rao Mohammed Al-Subaie, Abeer Yousuf Al-Jindan, Reem Papayya Balakrishna, Janardhana Kanchi Ravi, Padma Veeraraghavan, Vishnu Priya Arumugam Pillai, Aruthra Gollapalli, Shiva Shankar Reddy Palpath Joseph, Joel Surapaneni, Krishna Mohan Heliyon Article BACKGROUND: Identification and assessment of therapeutic potential of natural products derived from medicinal plants have led to the discovery of innovative and economical drugs to treat several diseases, including chronic wounds. In vitro cell based scratch assay is an appropriate and inexpensive method for initial understanding of wound healing potential of medicinal plant extracts. The current study was aimed at investigating the wound healing capacity of Aristolochia saccata leaf extract by using scratch assay as a primary model, where proliferative and migratory capabilities of test compounds could be monitored through microscopy studies. A. saccata is an evergreen climbing shrub belongs to the family Aristolochiaceae. METHODS: Methanolic extraction of the plant material was done using Soxhlet apparatus and the cytotoxicity of the extract on L929 cells was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. L929 is a human fibroblast cell line. In vitro scratch assay was performed to evaluate the wound healing properties of A. saccata leaf extract and possible mechanism of action was analyzed by flow cytometric expression studies of an extracellular matrix (ECM) factor, collagen type-1. RESULTS: MTT assay revealed that A. saccata leaf extract had no cytotoxic effect on the cells and at higher concentrations, the extract showed mild toxicity resulting in the death of just 2.88% cells. Scratch assay showed 34.05%, 70.00%, 93.52% wound closure at 12hrs, 24hrs and 48hrs of incubation respectively. These results were similar compared to positive control which showed 37.60, 56.41 and 99.05% of wound closure. Further, flow cytometry-based studies revealed that the A. saccata leaf extract induced the expression of ECM remodelling factor collagen-1. CONCLUSION: Our study revealed the wound healing capabilities of A. saccata In vitro. Hence, A. saccata could be recommended as a potential source of wound healing agents. Elsevier 2019-05-20 /pmc/articles/PMC6529694/ /pubmed/31193473 http://dx.doi.org/10.1016/j.heliyon.2019.e01648 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Bolla, Srinivasa Rao
Mohammed Al-Subaie, Abeer
Yousuf Al-Jindan, Reem
Papayya Balakrishna, Janardhana
Kanchi Ravi, Padma
Veeraraghavan, Vishnu Priya
Arumugam Pillai, Aruthra
Gollapalli, Shiva Shankar Reddy
Palpath Joseph, Joel
Surapaneni, Krishna Mohan
In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression
title In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression
title_full In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression
title_fullStr In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression
title_full_unstemmed In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression
title_short In vitro wound healing potency of methanolic leaf extract of Aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression
title_sort in vitro wound healing potency of methanolic leaf extract of aristolochia saccata is possibly mediated by its stimulatory effect on collagen-1 expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529694/
https://www.ncbi.nlm.nih.gov/pubmed/31193473
http://dx.doi.org/10.1016/j.heliyon.2019.e01648
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