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Comparative Genome Characterization of a Petroleum-Degrading Bacillus subtilis Strain DM2

The complete genome sequence of Bacillus subtilis strain DM2 isolated from petroleum-contaminated soil on the Tibetan Plateau was determined. The genome of strain DM2 consists of a circular chromosome of 4,238,631 bp for 4458 protein-coding genes and a plasmid of 84,240 bp coding for 103 genes. Thir...

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Autores principales: Li, Shi-Weng, Liu, Meng-Yuan, Yang, Rui-Qi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6530121/
https://www.ncbi.nlm.nih.gov/pubmed/31205931
http://dx.doi.org/10.1155/2019/7410823
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author Li, Shi-Weng
Liu, Meng-Yuan
Yang, Rui-Qi
author_facet Li, Shi-Weng
Liu, Meng-Yuan
Yang, Rui-Qi
author_sort Li, Shi-Weng
collection PubMed
description The complete genome sequence of Bacillus subtilis strain DM2 isolated from petroleum-contaminated soil on the Tibetan Plateau was determined. The genome of strain DM2 consists of a circular chromosome of 4,238,631 bp for 4458 protein-coding genes and a plasmid of 84,240 bp coding for 103 genes. Thirty-four genomic islands coding for 330 proteins and 5 prophages are found in the genome. The DDH value shows that strain DM2 belongs to B. subtilis subsp. subtilis subspecies, but significant variations of the genome are also present. Comparative analysis showed that the genome of strain DM2 encodes some strain-specific proteins in comparison with B. subtilis subsp. subtilis str. 168, such as carboxymuconolactone decarboxylase family protein, gfo/Idh/MocA family oxidoreductases, GlsB/YeaQ/YmgE family stress response membrane protein, HlyC/CorC family transporters, LLM class flavin-dependent oxidoreductase, and LPXTG cell wall anchor domain-containing protein. Most of the common strain-specific proteins in DM2 and MJ01 strains, or proteins unique to DM2 strain, are involved in the pathways related to stress response, signaling, and hydrocarbon degradation. Furthermore, the strain DM2 genome contains 122 genes coding for developed two-component systems and 138 genes coding for ABC transporter systems. The prominent features of the strain DM2 genome reflect the evolutionary fitness of this strain to harsh conditions and hydrocarbon utilization.
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spelling pubmed-65301212019-06-16 Comparative Genome Characterization of a Petroleum-Degrading Bacillus subtilis Strain DM2 Li, Shi-Weng Liu, Meng-Yuan Yang, Rui-Qi Int J Genomics Research Article The complete genome sequence of Bacillus subtilis strain DM2 isolated from petroleum-contaminated soil on the Tibetan Plateau was determined. The genome of strain DM2 consists of a circular chromosome of 4,238,631 bp for 4458 protein-coding genes and a plasmid of 84,240 bp coding for 103 genes. Thirty-four genomic islands coding for 330 proteins and 5 prophages are found in the genome. The DDH value shows that strain DM2 belongs to B. subtilis subsp. subtilis subspecies, but significant variations of the genome are also present. Comparative analysis showed that the genome of strain DM2 encodes some strain-specific proteins in comparison with B. subtilis subsp. subtilis str. 168, such as carboxymuconolactone decarboxylase family protein, gfo/Idh/MocA family oxidoreductases, GlsB/YeaQ/YmgE family stress response membrane protein, HlyC/CorC family transporters, LLM class flavin-dependent oxidoreductase, and LPXTG cell wall anchor domain-containing protein. Most of the common strain-specific proteins in DM2 and MJ01 strains, or proteins unique to DM2 strain, are involved in the pathways related to stress response, signaling, and hydrocarbon degradation. Furthermore, the strain DM2 genome contains 122 genes coding for developed two-component systems and 138 genes coding for ABC transporter systems. The prominent features of the strain DM2 genome reflect the evolutionary fitness of this strain to harsh conditions and hydrocarbon utilization. Hindawi 2019-05-08 /pmc/articles/PMC6530121/ /pubmed/31205931 http://dx.doi.org/10.1155/2019/7410823 Text en Copyright © 2019 Shi-Weng Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Shi-Weng
Liu, Meng-Yuan
Yang, Rui-Qi
Comparative Genome Characterization of a Petroleum-Degrading Bacillus subtilis Strain DM2
title Comparative Genome Characterization of a Petroleum-Degrading Bacillus subtilis Strain DM2
title_full Comparative Genome Characterization of a Petroleum-Degrading Bacillus subtilis Strain DM2
title_fullStr Comparative Genome Characterization of a Petroleum-Degrading Bacillus subtilis Strain DM2
title_full_unstemmed Comparative Genome Characterization of a Petroleum-Degrading Bacillus subtilis Strain DM2
title_short Comparative Genome Characterization of a Petroleum-Degrading Bacillus subtilis Strain DM2
title_sort comparative genome characterization of a petroleum-degrading bacillus subtilis strain dm2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6530121/
https://www.ncbi.nlm.nih.gov/pubmed/31205931
http://dx.doi.org/10.1155/2019/7410823
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