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Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro

Exosomes and other extracellular vesicles are key players in cell-to-cell communication, and it has been proposed that they are involved in different aspects of the response to ionizing radiation, including transmitting the radiation-induced bystander effect and mediating radioresistance. The functi...

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Autores principales: Abramowicz, Agata, Wojakowska, Anna, Marczak, Lukasz, Lysek-Gladysinska, Malgorzata, Smolarz, Mateusz, Story, Michael D, Polanska, Joanna, Widlak, Piotr, Pietrowska, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6530623/
https://www.ncbi.nlm.nih.gov/pubmed/30805606
http://dx.doi.org/10.1093/jrr/rrz001
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author Abramowicz, Agata
Wojakowska, Anna
Marczak, Lukasz
Lysek-Gladysinska, Malgorzata
Smolarz, Mateusz
Story, Michael D
Polanska, Joanna
Widlak, Piotr
Pietrowska, Monika
author_facet Abramowicz, Agata
Wojakowska, Anna
Marczak, Lukasz
Lysek-Gladysinska, Malgorzata
Smolarz, Mateusz
Story, Michael D
Polanska, Joanna
Widlak, Piotr
Pietrowska, Monika
author_sort Abramowicz, Agata
collection PubMed
description Exosomes and other extracellular vesicles are key players in cell-to-cell communication, and it has been proposed that they are involved in different aspects of the response to ionizing radiation, including transmitting the radiation-induced bystander effect and mediating radioresistance. The functional role of exosomes depends on their molecular cargo, including proteome content. Here we aimed to establish the proteome profile of exosomes released in vitro by irradiated UM-SCC6 cells derived from human head-and-neck cancer and to identify processes associated with radiation-affected proteins. Exosomes and other small extracellular vesicles were purified by size-exclusion chromatography from cell culture media collected 24 h after irradiation of cells with a single 2, 4 or 8 Gy dose, and then proteins were identified using a shotgun LC-MS/MS approach. Exosome-specific proteins encoded by 1217 unique genes were identified. There were 472 proteins whose abundance in exosomes was significantly affected by radiation (at any dose), including 425 upregulated and 47 downregulated species. The largest group of proteins affected by radiation (369 species) included those with increased abundance at all radiation doses (≥2 Gy). Several gene ontology terms were associated with radiation-affected exosome proteins. Among overrepresented processes were those involved in the response to radiation, the metabolism of radical oxygen species, DNA repair, chromatin packaging, and protein folding. Hence, the protein content of exosomes released by irradiated cells indicates their actual role in mediating the response to ionizing radiation.
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spelling pubmed-65306232019-05-28 Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro Abramowicz, Agata Wojakowska, Anna Marczak, Lukasz Lysek-Gladysinska, Malgorzata Smolarz, Mateusz Story, Michael D Polanska, Joanna Widlak, Piotr Pietrowska, Monika J Radiat Res Regular Paper Exosomes and other extracellular vesicles are key players in cell-to-cell communication, and it has been proposed that they are involved in different aspects of the response to ionizing radiation, including transmitting the radiation-induced bystander effect and mediating radioresistance. The functional role of exosomes depends on their molecular cargo, including proteome content. Here we aimed to establish the proteome profile of exosomes released in vitro by irradiated UM-SCC6 cells derived from human head-and-neck cancer and to identify processes associated with radiation-affected proteins. Exosomes and other small extracellular vesicles were purified by size-exclusion chromatography from cell culture media collected 24 h after irradiation of cells with a single 2, 4 or 8 Gy dose, and then proteins were identified using a shotgun LC-MS/MS approach. Exosome-specific proteins encoded by 1217 unique genes were identified. There were 472 proteins whose abundance in exosomes was significantly affected by radiation (at any dose), including 425 upregulated and 47 downregulated species. The largest group of proteins affected by radiation (369 species) included those with increased abundance at all radiation doses (≥2 Gy). Several gene ontology terms were associated with radiation-affected exosome proteins. Among overrepresented processes were those involved in the response to radiation, the metabolism of radical oxygen species, DNA repair, chromatin packaging, and protein folding. Hence, the protein content of exosomes released by irradiated cells indicates their actual role in mediating the response to ionizing radiation. Oxford University Press 2019-05 2019-02-26 /pmc/articles/PMC6530623/ /pubmed/30805606 http://dx.doi.org/10.1093/jrr/rrz001 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial reuse, please contact journals.permissions@oup.com
spellingShingle Regular Paper
Abramowicz, Agata
Wojakowska, Anna
Marczak, Lukasz
Lysek-Gladysinska, Malgorzata
Smolarz, Mateusz
Story, Michael D
Polanska, Joanna
Widlak, Piotr
Pietrowska, Monika
Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro
title Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro
title_full Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro
title_fullStr Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro
title_full_unstemmed Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro
title_short Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro
title_sort ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro
topic Regular Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6530623/
https://www.ncbi.nlm.nih.gov/pubmed/30805606
http://dx.doi.org/10.1093/jrr/rrz001
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