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Bone marrow stromal cells interaction with titanium; Effects of composition and surface modification

Inflammation and implant loosening are major concerns when using titanium implants for hard tissue engineering applications. Surface modification is one of the promising tools to enhance tissue-material integration in metallic implants. Here, we used anodization technique to modify the surface of co...

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Autores principales: Duvvuru, Murali Krishna, Han, Weiguo, Chowdhury, Prantik Roy, Vahabzadeh, Sahar, Sciammarella, Federico, Elsawa, Sherine F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6530826/
https://www.ncbi.nlm.nih.gov/pubmed/31116747
http://dx.doi.org/10.1371/journal.pone.0216087
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author Duvvuru, Murali Krishna
Han, Weiguo
Chowdhury, Prantik Roy
Vahabzadeh, Sahar
Sciammarella, Federico
Elsawa, Sherine F.
author_facet Duvvuru, Murali Krishna
Han, Weiguo
Chowdhury, Prantik Roy
Vahabzadeh, Sahar
Sciammarella, Federico
Elsawa, Sherine F.
author_sort Duvvuru, Murali Krishna
collection PubMed
description Inflammation and implant loosening are major concerns when using titanium implants for hard tissue engineering applications. Surface modification is one of the promising tools to enhance tissue-material integration in metallic implants. Here, we used anodization technique to modify the surface of commercially pure titanium (CP-Ti) and titanium alloy (Ti-6Al-4V) samples. Our results show that electrolyte composition, anodization time and voltage dictated the formation of well-organized nanotubes. Although electrolyte containing HF in water resulted in nanotube formation on Ti, the presence of NH4F and ethylene glycol was necessary for successful nanotube formation on Ti-6Al-4V. Upon examination of the interaction of bone marrow stromal cells (BMSCs) with the modified samples, we found that Ti-6Al-4V without nanotubes induced cell proliferation and cluster of differentiation 40 ligand (CD40L) expression which facilitates B-cell activation to promote early bone healing. However, the expression of glioma associated protein 2 (GLI2), which regulates CD40L, was reduced in Ti-6Al-4V and the presence of nanotubes further reduced its expression. The inflammatory cytokine interleukin-6 (IL-6) expression was reduced by nanotube presence on Ti. These results suggest that Ti-6Al-4V with nanotubes may be suitable implants because they have no effect on BMSC growth and inflammation.
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spelling pubmed-65308262019-05-31 Bone marrow stromal cells interaction with titanium; Effects of composition and surface modification Duvvuru, Murali Krishna Han, Weiguo Chowdhury, Prantik Roy Vahabzadeh, Sahar Sciammarella, Federico Elsawa, Sherine F. PLoS One Research Article Inflammation and implant loosening are major concerns when using titanium implants for hard tissue engineering applications. Surface modification is one of the promising tools to enhance tissue-material integration in metallic implants. Here, we used anodization technique to modify the surface of commercially pure titanium (CP-Ti) and titanium alloy (Ti-6Al-4V) samples. Our results show that electrolyte composition, anodization time and voltage dictated the formation of well-organized nanotubes. Although electrolyte containing HF in water resulted in nanotube formation on Ti, the presence of NH4F and ethylene glycol was necessary for successful nanotube formation on Ti-6Al-4V. Upon examination of the interaction of bone marrow stromal cells (BMSCs) with the modified samples, we found that Ti-6Al-4V without nanotubes induced cell proliferation and cluster of differentiation 40 ligand (CD40L) expression which facilitates B-cell activation to promote early bone healing. However, the expression of glioma associated protein 2 (GLI2), which regulates CD40L, was reduced in Ti-6Al-4V and the presence of nanotubes further reduced its expression. The inflammatory cytokine interleukin-6 (IL-6) expression was reduced by nanotube presence on Ti. These results suggest that Ti-6Al-4V with nanotubes may be suitable implants because they have no effect on BMSC growth and inflammation. Public Library of Science 2019-05-22 /pmc/articles/PMC6530826/ /pubmed/31116747 http://dx.doi.org/10.1371/journal.pone.0216087 Text en © 2019 Duvvuru et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Duvvuru, Murali Krishna
Han, Weiguo
Chowdhury, Prantik Roy
Vahabzadeh, Sahar
Sciammarella, Federico
Elsawa, Sherine F.
Bone marrow stromal cells interaction with titanium; Effects of composition and surface modification
title Bone marrow stromal cells interaction with titanium; Effects of composition and surface modification
title_full Bone marrow stromal cells interaction with titanium; Effects of composition and surface modification
title_fullStr Bone marrow stromal cells interaction with titanium; Effects of composition and surface modification
title_full_unstemmed Bone marrow stromal cells interaction with titanium; Effects of composition and surface modification
title_short Bone marrow stromal cells interaction with titanium; Effects of composition and surface modification
title_sort bone marrow stromal cells interaction with titanium; effects of composition and surface modification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6530826/
https://www.ncbi.nlm.nih.gov/pubmed/31116747
http://dx.doi.org/10.1371/journal.pone.0216087
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