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Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker
INTRODUCTION: Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method. METHODS: A total of 1521 stool samples were collected from the stud...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Makerere Medical School
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6531938/ https://www.ncbi.nlm.nih.gov/pubmed/31148960 http://dx.doi.org/10.4314/ahs.v19i1.6 |
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author | Shahnazi, Mojtaba Mehrizi, Majid Zarezadeh Alizadeh, Safar Ali Heydarian, Peyman Saraei, Mehrzad Alipour, Mahmood Hajialilo, Elham |
author_facet | Shahnazi, Mojtaba Mehrizi, Majid Zarezadeh Alizadeh, Safar Ali Heydarian, Peyman Saraei, Mehrzad Alipour, Mahmood Hajialilo, Elham |
author_sort | Shahnazi, Mojtaba |
collection | PubMed |
description | INTRODUCTION: Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method. METHODS: A total of 1521 stool samples were collected from the study individuals. Initially, the identification of hymenolepis nana was confirmed by parasitological method including direct wet-mount and formalin-ethyl acetate concentration methods. Afterward, PCR-sequencing analysis of ribosomal ITS2 fragment was targeted to investigate the molecular identification of the parasite. RESULTS: Overall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration. All ten isolates were succefully amplified by PCR and further sequenced. The determined sequences were deposited in GenBank under the accession numbers MH337810 -MH337819. CONCLUSION: Our results clarified the presence of H. nana among the patients in the study areas. In addition, the molecular technique could be accessible when the human eggs are the only sources available to identify and diagnose the parasite. |
format | Online Article Text |
id | pubmed-6531938 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Makerere Medical School |
record_format | MEDLINE/PubMed |
spelling | pubmed-65319382019-05-30 Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker Shahnazi, Mojtaba Mehrizi, Majid Zarezadeh Alizadeh, Safar Ali Heydarian, Peyman Saraei, Mehrzad Alipour, Mahmood Hajialilo, Elham Afr Health Sci Articles INTRODUCTION: Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method. METHODS: A total of 1521 stool samples were collected from the study individuals. Initially, the identification of hymenolepis nana was confirmed by parasitological method including direct wet-mount and formalin-ethyl acetate concentration methods. Afterward, PCR-sequencing analysis of ribosomal ITS2 fragment was targeted to investigate the molecular identification of the parasite. RESULTS: Overall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration. All ten isolates were succefully amplified by PCR and further sequenced. The determined sequences were deposited in GenBank under the accession numbers MH337810 -MH337819. CONCLUSION: Our results clarified the presence of H. nana among the patients in the study areas. In addition, the molecular technique could be accessible when the human eggs are the only sources available to identify and diagnose the parasite. Makerere Medical School 2019-03 /pmc/articles/PMC6531938/ /pubmed/31148960 http://dx.doi.org/10.4314/ahs.v19i1.6 Text en © 2019 Shahnazi et al. Licensee African Health Sciences. This is an Open Access article distributed under the terms of the Creative commons Attribution License (https://creativecommons.org/licenses/BY/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Shahnazi, Mojtaba Mehrizi, Majid Zarezadeh Alizadeh, Safar Ali Heydarian, Peyman Saraei, Mehrzad Alipour, Mahmood Hajialilo, Elham Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker |
title | Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker |
title_full | Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker |
title_fullStr | Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker |
title_full_unstemmed | Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker |
title_short | Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker |
title_sort | molecular characterization of hymenolepis nana based on nuclear rdna its2 gene marker |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6531938/ https://www.ncbi.nlm.nih.gov/pubmed/31148960 http://dx.doi.org/10.4314/ahs.v19i1.6 |
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