A screening method to identify efficient sgRNAs in Arabidopsis, used in conjunction with cell-specific lignin reduction

BACKGROUND: Single guide RNA (sgRNA) selection is important for the efficiency of CRISPR/Cas9-mediated genome editing. However, in plants, the rules governing selection are not well established. RESULTS: We developed a facile transient assay to screen sgRNA efficiency. We then used it to test top-pe...

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Detalles Bibliográficos
Autores principales: Liang, Yan, Eudes, Aymerick, Yogiswara, Sasha, Jing, Beibei, Benites, Veronica T., Yamanaka, Reo, Cheng-Yue, Clarabelle, Baidoo, Edward E., Mortimer, Jenny C., Scheller, Henrik V., Loqué, Dominique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532251/
https://www.ncbi.nlm.nih.gov/pubmed/31143243
http://dx.doi.org/10.1186/s13068-019-1467-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532251/
https://www.ncbi.nlm.nih.gov/pubmed/31143243
http://dx.doi.org/10.1186/s13068-019-1467-y

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