A photo-cleavable surfactant for top-down proteomics

We report the identification of a photo-cleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo) that can be rapidly degraded upon UV irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to SDS and is mass spectrometry (MS)-compatible. Import...

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Detalles Bibliográficos
Autores principales: Brown, Kyle A., Chen, Bifan, Guardado-Alvarez, Tania M., Lin, Ziqing, Hwang, Leekyoung, Ayaz-Guner, Serife, Jin, Song, Ge, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532422/
https://www.ncbi.nlm.nih.gov/pubmed/30988469
http://dx.doi.org/10.1038/s41592-019-0391-1
Descripción
Sumario:We report the identification of a photo-cleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo) that can be rapidly degraded upon UV irradiation, for top-down proteomics. Azo can effectively solubilize proteins with performance comparable to SDS and is mass spectrometry (MS)-compatible. Importantly, Azo-aided top-down proteomics enables the solubilization of membrane proteins for comprehensive characterization of post-translational modifications. Moreover, Azo is simple to synthesize and can be used as a general SDS replacement in SDS-PAGE.

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