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Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse
The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. A...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532964/ https://www.ncbi.nlm.nih.gov/pubmed/31120961 http://dx.doi.org/10.1371/journal.pone.0217391 |
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author | Almeida, Daniela Santos Paz, Lucas Nogueira de Oliveira, Daiana Santos Silva, Danielle Nascimento Ristow, Paula Hamond, Camila Costa, Federico Portela, Ricardo Wagner Estrela-Lima, Alessandra Pinna, Melissa Hanzen |
author_facet | Almeida, Daniela Santos Paz, Lucas Nogueira de Oliveira, Daiana Santos Silva, Danielle Nascimento Ristow, Paula Hamond, Camila Costa, Federico Portela, Ricardo Wagner Estrela-Lima, Alessandra Pinna, Melissa Hanzen |
author_sort | Almeida, Daniela Santos |
collection | PubMed |
description | The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis. |
format | Online Article Text |
id | pubmed-6532964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-65329642019-06-05 Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse Almeida, Daniela Santos Paz, Lucas Nogueira de Oliveira, Daiana Santos Silva, Danielle Nascimento Ristow, Paula Hamond, Camila Costa, Federico Portela, Ricardo Wagner Estrela-Lima, Alessandra Pinna, Melissa Hanzen PLoS One Research Article The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis. Public Library of Science 2019-05-23 /pmc/articles/PMC6532964/ /pubmed/31120961 http://dx.doi.org/10.1371/journal.pone.0217391 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Almeida, Daniela Santos Paz, Lucas Nogueira de Oliveira, Daiana Santos Silva, Danielle Nascimento Ristow, Paula Hamond, Camila Costa, Federico Portela, Ricardo Wagner Estrela-Lima, Alessandra Pinna, Melissa Hanzen Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse |
title | Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse |
title_full | Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse |
title_fullStr | Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse |
title_full_unstemmed | Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse |
title_short | Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse |
title_sort | investigation of chronic infection by leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532964/ https://www.ncbi.nlm.nih.gov/pubmed/31120961 http://dx.doi.org/10.1371/journal.pone.0217391 |
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