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Silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse

Understanding the molecular mechanism of glaucoma and development of neuroprotectants is significantly hindered by the lack of a reliable animal model that accurately recapitulates human glaucoma. Here, we sought to develop a mouse model for the secondary glaucoma that is often observed in humans af...

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Autores principales: Zhang, Jie, Li, Liang, Huang, Haoliang, Fang, Fang, Webber, Hannah C, Zhuang, Pei, Liu, Liang, Dalal, Roopa, Tang, Peter H, Mahajan, Vinit B, Sun, Yang, Li, Shaohua, Zhang, Mingchang, Goldberg, Jeffrey L, Hu, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6533060/
https://www.ncbi.nlm.nih.gov/pubmed/31090540
http://dx.doi.org/10.7554/eLife.45881
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author Zhang, Jie
Li, Liang
Huang, Haoliang
Fang, Fang
Webber, Hannah C
Zhuang, Pei
Liu, Liang
Dalal, Roopa
Tang, Peter H
Mahajan, Vinit B
Sun, Yang
Li, Shaohua
Zhang, Mingchang
Goldberg, Jeffrey L
Hu, Yang
author_facet Zhang, Jie
Li, Liang
Huang, Haoliang
Fang, Fang
Webber, Hannah C
Zhuang, Pei
Liu, Liang
Dalal, Roopa
Tang, Peter H
Mahajan, Vinit B
Sun, Yang
Li, Shaohua
Zhang, Mingchang
Goldberg, Jeffrey L
Hu, Yang
author_sort Zhang, Jie
collection PubMed
description Understanding the molecular mechanism of glaucoma and development of neuroprotectants is significantly hindered by the lack of a reliable animal model that accurately recapitulates human glaucoma. Here, we sought to develop a mouse model for the secondary glaucoma that is often observed in humans after silicone oil (SO) blocks the pupil or migrates into the anterior chamber following vitreoretinal surgery. We observed significant intraocular pressure (IOP) elevation after intracameral injection of SO, and that SO removal allows IOP to return quickly to normal. This simple, inducible and reversible mouse ocular hypertension model shows dynamic changes of visual function that correlate with progressive retinal ganglion cell (RGC) loss and axon degeneration. It may be applicable with only minor modifications to a range of animal species in which it will generate stable, robust IOP elevation and significant neurodegeneration that will facilitate selection of neuroprotectants and investigating the pathogenesis of ocular hypertension-induced glaucoma.
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spelling pubmed-65330602019-05-28 Silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse Zhang, Jie Li, Liang Huang, Haoliang Fang, Fang Webber, Hannah C Zhuang, Pei Liu, Liang Dalal, Roopa Tang, Peter H Mahajan, Vinit B Sun, Yang Li, Shaohua Zhang, Mingchang Goldberg, Jeffrey L Hu, Yang eLife Neuroscience Understanding the molecular mechanism of glaucoma and development of neuroprotectants is significantly hindered by the lack of a reliable animal model that accurately recapitulates human glaucoma. Here, we sought to develop a mouse model for the secondary glaucoma that is often observed in humans after silicone oil (SO) blocks the pupil or migrates into the anterior chamber following vitreoretinal surgery. We observed significant intraocular pressure (IOP) elevation after intracameral injection of SO, and that SO removal allows IOP to return quickly to normal. This simple, inducible and reversible mouse ocular hypertension model shows dynamic changes of visual function that correlate with progressive retinal ganglion cell (RGC) loss and axon degeneration. It may be applicable with only minor modifications to a range of animal species in which it will generate stable, robust IOP elevation and significant neurodegeneration that will facilitate selection of neuroprotectants and investigating the pathogenesis of ocular hypertension-induced glaucoma. eLife Sciences Publications, Ltd 2019-05-15 /pmc/articles/PMC6533060/ /pubmed/31090540 http://dx.doi.org/10.7554/eLife.45881 Text en © 2019, Zhang et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Zhang, Jie
Li, Liang
Huang, Haoliang
Fang, Fang
Webber, Hannah C
Zhuang, Pei
Liu, Liang
Dalal, Roopa
Tang, Peter H
Mahajan, Vinit B
Sun, Yang
Li, Shaohua
Zhang, Mingchang
Goldberg, Jeffrey L
Hu, Yang
Silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse
title Silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse
title_full Silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse
title_fullStr Silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse
title_full_unstemmed Silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse
title_short Silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse
title_sort silicone oil-induced ocular hypertension and glaucomatous neurodegeneration in mouse
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6533060/
https://www.ncbi.nlm.nih.gov/pubmed/31090540
http://dx.doi.org/10.7554/eLife.45881
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