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Matrix Metalloproteinase 2 Knockdown Suppresses the Proliferation of HepG2 and Huh7 Cells and Enhances the Cisplatin Effect
BACKGROUND: This study evaluated the functions of matrix metalloproteinase 2 (MMP2) in hepatocellular carcinoma (HCC) cells and assessed the effects of MMP2 on HCC cell sensitivity to cisplatin. METHODOLOGY: HepG2 and Huh7 cells were cultured. A pre-experiment was performed to explore the optimal tr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534103/ https://www.ncbi.nlm.nih.gov/pubmed/31157304 http://dx.doi.org/10.1515/med-2019-0039 |
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author | Liu, Jiangwei Li, Xiaocheng Huang, Jianzhao Liu, Yan |
author_facet | Liu, Jiangwei Li, Xiaocheng Huang, Jianzhao Liu, Yan |
author_sort | Liu, Jiangwei |
collection | PubMed |
description | BACKGROUND: This study evaluated the functions of matrix metalloproteinase 2 (MMP2) in hepatocellular carcinoma (HCC) cells and assessed the effects of MMP2 on HCC cell sensitivity to cisplatin. METHODOLOGY: HepG2 and Huh7 cells were cultured. A pre-experiment was performed to explore the optimal transduction conditions of the MMP2-siRNA lentivirus (si-MMP2). Quantitative real-time PCR and western blot assays were performed to measure the expression levels of MMP2 in HepG2 and Huh7 cells. An MTT assay was used to evaluate cell proliferation, and flow cytometry analysis was applied to examine cell apoptosis. A Transwell assay was carried out to assess cell invasion. RESULTS: The optimal virus:cell ratio was 100 multiplicity of infection (MOI) for both cells, and the optimal transduction times for HepG2 and Huh7 cells were 48 h and 72 h, respectively. MMP2 knockdown significantly decreased the mRNA and protein levels of MMP2 in both cell lines (P<0.01). MMP2 knockdown significantly decreased the proliferation and increased the apoptosis of HepG2 and Huh7 cells (P<0.01). Co-treatment with si-MMP2 and cisplatin significantly increased the sensitivity of HepG2 and Huh7 cells to cisplatin (P<0.01). CONCLUSION: MMP2 may act as an oncogene and may be a potential therapeutic target in HCC. |
format | Online Article Text |
id | pubmed-6534103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-65341032019-05-31 Matrix Metalloproteinase 2 Knockdown Suppresses the Proliferation of HepG2 and Huh7 Cells and Enhances the Cisplatin Effect Liu, Jiangwei Li, Xiaocheng Huang, Jianzhao Liu, Yan Open Med (Wars) Research Article BACKGROUND: This study evaluated the functions of matrix metalloproteinase 2 (MMP2) in hepatocellular carcinoma (HCC) cells and assessed the effects of MMP2 on HCC cell sensitivity to cisplatin. METHODOLOGY: HepG2 and Huh7 cells were cultured. A pre-experiment was performed to explore the optimal transduction conditions of the MMP2-siRNA lentivirus (si-MMP2). Quantitative real-time PCR and western blot assays were performed to measure the expression levels of MMP2 in HepG2 and Huh7 cells. An MTT assay was used to evaluate cell proliferation, and flow cytometry analysis was applied to examine cell apoptosis. A Transwell assay was carried out to assess cell invasion. RESULTS: The optimal virus:cell ratio was 100 multiplicity of infection (MOI) for both cells, and the optimal transduction times for HepG2 and Huh7 cells were 48 h and 72 h, respectively. MMP2 knockdown significantly decreased the mRNA and protein levels of MMP2 in both cell lines (P<0.01). MMP2 knockdown significantly decreased the proliferation and increased the apoptosis of HepG2 and Huh7 cells (P<0.01). Co-treatment with si-MMP2 and cisplatin significantly increased the sensitivity of HepG2 and Huh7 cells to cisplatin (P<0.01). CONCLUSION: MMP2 may act as an oncogene and may be a potential therapeutic target in HCC. De Gruyter 2019-05-17 /pmc/articles/PMC6534103/ /pubmed/31157304 http://dx.doi.org/10.1515/med-2019-0039 Text en © 2019 Jiangwei Liu et al., published by De Gruyter http://creativecommons.org/licenses/by-nc-nd/4.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. |
spellingShingle | Research Article Liu, Jiangwei Li, Xiaocheng Huang, Jianzhao Liu, Yan Matrix Metalloproteinase 2 Knockdown Suppresses the Proliferation of HepG2 and Huh7 Cells and Enhances the Cisplatin Effect |
title | Matrix Metalloproteinase 2 Knockdown Suppresses the Proliferation of HepG2 and Huh7 Cells and Enhances the Cisplatin Effect |
title_full | Matrix Metalloproteinase 2 Knockdown Suppresses the Proliferation of HepG2 and Huh7 Cells and Enhances the Cisplatin Effect |
title_fullStr | Matrix Metalloproteinase 2 Knockdown Suppresses the Proliferation of HepG2 and Huh7 Cells and Enhances the Cisplatin Effect |
title_full_unstemmed | Matrix Metalloproteinase 2 Knockdown Suppresses the Proliferation of HepG2 and Huh7 Cells and Enhances the Cisplatin Effect |
title_short | Matrix Metalloproteinase 2 Knockdown Suppresses the Proliferation of HepG2 and Huh7 Cells and Enhances the Cisplatin Effect |
title_sort | matrix metalloproteinase 2 knockdown suppresses the proliferation of hepg2 and huh7 cells and enhances the cisplatin effect |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534103/ https://www.ncbi.nlm.nih.gov/pubmed/31157304 http://dx.doi.org/10.1515/med-2019-0039 |
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