Cargando…

Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated

Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies...

Descripción completa

Detalles Bibliográficos
Autores principales: Sneeboer, Marjolein A. M., Snijders, Gijsje J. L. J., Berdowski, Woutje M., Fernández-Andreu, Alba, van Mierlo, Hans C., Berdenis van Berlekom, Amber, Litjens, Manja, Kahn, René S., Hol, Elly M., de Witte, Lot D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534632/
https://www.ncbi.nlm.nih.gov/pubmed/31127084
http://dx.doi.org/10.1038/s41398-019-0490-x
_version_ 1783421455054143488
author Sneeboer, Marjolein A. M.
Snijders, Gijsje J. L. J.
Berdowski, Woutje M.
Fernández-Andreu, Alba
van Mierlo, Hans C.
Berdenis van Berlekom, Amber
Litjens, Manja
Kahn, René S.
Hol, Elly M.
de Witte, Lot D.
author_facet Sneeboer, Marjolein A. M.
Snijders, Gijsje J. L. J.
Berdowski, Woutje M.
Fernández-Andreu, Alba
van Mierlo, Hans C.
Berdenis van Berlekom, Amber
Litjens, Manja
Kahn, René S.
Hol, Elly M.
de Witte, Lot D.
author_sort Sneeboer, Marjolein A. M.
collection PubMed
description Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies have addressed the involvement of microglia in BD so far and a more detailed immune profiling of microglial activation is lacking. Here, we applied a multi-level approach to determine the activation state of microglia in BD post-mortem brain tissue. We did not find differences in microglial density, and mRNA expression of microglial markers in the medial frontal gyrus (MFG) of patients with BD. Furthermore, we performed in-depth characterization of human primary microglia isolated from fresh brain tissue of the MFG, superior temporal gyrus (STG), and thalamus (THA). Similarly, these ex vivo isolated microglia did not show elevated expression of inflammatory markers. Finally, challenging the isolated microglia with LPS did not result in an increased immune response in patients with BD compared to controls. In conclusion, our study shows that microglia in post-mortem brain tissue of patients with BD are not immune activated.
format Online
Article
Text
id pubmed-6534632
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-65346322019-05-30 Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated Sneeboer, Marjolein A. M. Snijders, Gijsje J. L. J. Berdowski, Woutje M. Fernández-Andreu, Alba van Mierlo, Hans C. Berdenis van Berlekom, Amber Litjens, Manja Kahn, René S. Hol, Elly M. de Witte, Lot D. Transl Psychiatry Article Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies have addressed the involvement of microglia in BD so far and a more detailed immune profiling of microglial activation is lacking. Here, we applied a multi-level approach to determine the activation state of microglia in BD post-mortem brain tissue. We did not find differences in microglial density, and mRNA expression of microglial markers in the medial frontal gyrus (MFG) of patients with BD. Furthermore, we performed in-depth characterization of human primary microglia isolated from fresh brain tissue of the MFG, superior temporal gyrus (STG), and thalamus (THA). Similarly, these ex vivo isolated microglia did not show elevated expression of inflammatory markers. Finally, challenging the isolated microglia with LPS did not result in an increased immune response in patients with BD compared to controls. In conclusion, our study shows that microglia in post-mortem brain tissue of patients with BD are not immune activated. Nature Publishing Group UK 2019-05-24 /pmc/articles/PMC6534632/ /pubmed/31127084 http://dx.doi.org/10.1038/s41398-019-0490-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sneeboer, Marjolein A. M.
Snijders, Gijsje J. L. J.
Berdowski, Woutje M.
Fernández-Andreu, Alba
van Mierlo, Hans C.
Berdenis van Berlekom, Amber
Litjens, Manja
Kahn, René S.
Hol, Elly M.
de Witte, Lot D.
Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated
title Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated
title_full Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated
title_fullStr Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated
title_full_unstemmed Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated
title_short Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated
title_sort microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534632/
https://www.ncbi.nlm.nih.gov/pubmed/31127084
http://dx.doi.org/10.1038/s41398-019-0490-x
work_keys_str_mv AT sneeboermarjoleinam microgliainpostmortembraintissueofpatientswithbipolardisorderarenotimmuneactivated
AT snijdersgijsjejlj microgliainpostmortembraintissueofpatientswithbipolardisorderarenotimmuneactivated
AT berdowskiwoutjem microgliainpostmortembraintissueofpatientswithbipolardisorderarenotimmuneactivated
AT fernandezandreualba microgliainpostmortembraintissueofpatientswithbipolardisorderarenotimmuneactivated
AT microgliainpostmortembraintissueofpatientswithbipolardisorderarenotimmuneactivated
AT vanmierlohansc microgliainpostmortembraintissueofpatientswithbipolardisorderarenotimmuneactivated
AT berdenisvanberlekomamber microgliainpostmortembraintissueofpatientswithbipolardisorderarenotimmuneactivated
AT litjensmanja microgliainpostmortembraintissueofpatientswithbipolardisorderarenotimmuneactivated
AT kahnrenes microgliainpostmortembraintissueofpatientswithbipolardisorderarenotimmuneactivated
AT holellym microgliainpostmortembraintissueofpatientswithbipolardisorderarenotimmuneactivated
AT dewittelotd microgliainpostmortembraintissueofpatientswithbipolardisorderarenotimmuneactivated