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In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time
BACKGROUND: Knowledge of microbiota composition, persistence, and transmission as well as the overall function of the bacterial community is important and may be linked to honey bee health. This study aimed to investigate the inter-individual variation in the gut microbiota in honey bee larvae and p...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534886/ https://www.ncbi.nlm.nih.gov/pubmed/31126234 http://dx.doi.org/10.1186/s12866-019-1490-y |
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author | Hroncova, Zuzana Killer, Jiri Hakl, Josef Titera, Dalibor Havlik, Jaroslav |
author_facet | Hroncova, Zuzana Killer, Jiri Hakl, Josef Titera, Dalibor Havlik, Jaroslav |
author_sort | Hroncova, Zuzana |
collection | PubMed |
description | BACKGROUND: Knowledge of microbiota composition, persistence, and transmission as well as the overall function of the bacterial community is important and may be linked to honey bee health. This study aimed to investigate the inter-individual variation in the gut microbiota in honey bee larvae and pupae. RESULTS: Individual larvae differed in the composition of major bacterial groups. In the majority of 5th instar bees, Firmicutes showed predominance (70%); however, after larval defecation and during pupation, the abundance decreased to 40%, in favour of Gammaproteobacteria. The 5th instar larvae hosted significantly more (P < 0.001) Firmicutes than black pupae. Power calculations revealed that 11 and 18 replicate-individuals, respectively, were required for the detection of significant differences (P < 0.05) in the Bacteroidetes and Firmicutes abundance between stages, while higher numbers of replicates were required for Actinobacteria (478 replicates) and Gammaproteobacteria (111 replicates). CONCLUSIONS: Although sample processing and extraction protocols may have had a significant influence, sampling is very important for studying the bee microbiome, and the importance of the number of individuals pooled in samples used for microbiome studies should not be underestimated. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-019-1490-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6534886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-65348862019-05-30 In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time Hroncova, Zuzana Killer, Jiri Hakl, Josef Titera, Dalibor Havlik, Jaroslav BMC Microbiol Research Article BACKGROUND: Knowledge of microbiota composition, persistence, and transmission as well as the overall function of the bacterial community is important and may be linked to honey bee health. This study aimed to investigate the inter-individual variation in the gut microbiota in honey bee larvae and pupae. RESULTS: Individual larvae differed in the composition of major bacterial groups. In the majority of 5th instar bees, Firmicutes showed predominance (70%); however, after larval defecation and during pupation, the abundance decreased to 40%, in favour of Gammaproteobacteria. The 5th instar larvae hosted significantly more (P < 0.001) Firmicutes than black pupae. Power calculations revealed that 11 and 18 replicate-individuals, respectively, were required for the detection of significant differences (P < 0.05) in the Bacteroidetes and Firmicutes abundance between stages, while higher numbers of replicates were required for Actinobacteria (478 replicates) and Gammaproteobacteria (111 replicates). CONCLUSIONS: Although sample processing and extraction protocols may have had a significant influence, sampling is very important for studying the bee microbiome, and the importance of the number of individuals pooled in samples used for microbiome studies should not be underestimated. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-019-1490-y) contains supplementary material, which is available to authorized users. BioMed Central 2019-05-24 /pmc/articles/PMC6534886/ /pubmed/31126234 http://dx.doi.org/10.1186/s12866-019-1490-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Hroncova, Zuzana Killer, Jiri Hakl, Josef Titera, Dalibor Havlik, Jaroslav In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time |
title | In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time |
title_full | In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time |
title_fullStr | In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time |
title_full_unstemmed | In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time |
title_short | In-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time |
title_sort | in-hive variation of the gut microbial composition of honey bee larvae and pupae from the same oviposition time |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534886/ https://www.ncbi.nlm.nih.gov/pubmed/31126234 http://dx.doi.org/10.1186/s12866-019-1490-y |
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