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Determining the Need for Repeat Testing of Blood Ethanol Concentration: Evaluation of the Synchron Blood Ethyl Alcohol Assay Kit

BACKGROUND: In clinical laboratories, a common practice used to verify tests prior to reporting is repeat testing. Our objective was to evaluate the differences between the results of blood ethanol concentration (BEC) test repetitions and report on the role of repeat testing to prevent reporting of...

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Autores principales: Ustundağ, Yasemin, Huysal, Kağan, Eren, Sevim Eşmedere
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534961/
https://www.ncbi.nlm.nih.gov/pubmed/31156342
http://dx.doi.org/10.2478/jomb-2018-0032
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author Ustundağ, Yasemin
Huysal, Kağan
Eren, Sevim Eşmedere
author_facet Ustundağ, Yasemin
Huysal, Kağan
Eren, Sevim Eşmedere
author_sort Ustundağ, Yasemin
collection PubMed
description BACKGROUND: In clinical laboratories, a common practice used to verify tests prior to reporting is repeat testing. Our objective was to evaluate the differences between the results of blood ethanol concentration (BEC) test repetitions and report on the role of repeat testing to prevent reporting of incorrect results. METHODS: We conducted a retrospective study of data retrieved from the Bursa Yuksek Ihtisas Training and Research Hospital’s document management system by calculating the percentage change between repeated BEC test runs. To assess for clinical relevance, the bias between two results from the same sample was compared using the 1988 Clinical Laboratory Improvement Amendments’ (CLIA) proficiency testing allowable total error (TEa) limits. RESULTS: From a total of 1,627 BEC tests performed between January 2017 and January 2018, 70% (1,133) were repeat tested. Of these, 830 resulted in BECs between 0–5 mmol/L, of which 237 (28.5%) were above the 25% acceptable TEa. Two hundred seventy-six BEC test results were greater than >14 mmol/L, and there was a good consensus between the initial and repeat test results (99%). In this group, the mean bias was 0.0% (95%, CI = -9.8–9.8%). However, three of the repeat test results were considered significantly different. There were two discordant results in the 5–14 mmol/L ethanol level, and the mean bias was 2.1% (95%, CI = -15.0–19.1%). CONCLUSION: The majority of the repeated BEC test values were the same as the baseline value; therefore, there may be limited benefit in continuing such frequent repeated analyses.
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spelling pubmed-65349612019-05-31 Determining the Need for Repeat Testing of Blood Ethanol Concentration: Evaluation of the Synchron Blood Ethyl Alcohol Assay Kit Ustundağ, Yasemin Huysal, Kağan Eren, Sevim Eşmedere J Med Biochem Original Paper BACKGROUND: In clinical laboratories, a common practice used to verify tests prior to reporting is repeat testing. Our objective was to evaluate the differences between the results of blood ethanol concentration (BEC) test repetitions and report on the role of repeat testing to prevent reporting of incorrect results. METHODS: We conducted a retrospective study of data retrieved from the Bursa Yuksek Ihtisas Training and Research Hospital’s document management system by calculating the percentage change between repeated BEC test runs. To assess for clinical relevance, the bias between two results from the same sample was compared using the 1988 Clinical Laboratory Improvement Amendments’ (CLIA) proficiency testing allowable total error (TEa) limits. RESULTS: From a total of 1,627 BEC tests performed between January 2017 and January 2018, 70% (1,133) were repeat tested. Of these, 830 resulted in BECs between 0–5 mmol/L, of which 237 (28.5%) were above the 25% acceptable TEa. Two hundred seventy-six BEC test results were greater than >14 mmol/L, and there was a good consensus between the initial and repeat test results (99%). In this group, the mean bias was 0.0% (95%, CI = -9.8–9.8%). However, three of the repeat test results were considered significantly different. There were two discordant results in the 5–14 mmol/L ethanol level, and the mean bias was 2.1% (95%, CI = -15.0–19.1%). CONCLUSION: The majority of the repeated BEC test values were the same as the baseline value; therefore, there may be limited benefit in continuing such frequent repeated analyses. Sciendo 2019-05-11 /pmc/articles/PMC6534961/ /pubmed/31156342 http://dx.doi.org/10.2478/jomb-2018-0032 Text en © 2019 Yasemin Ustundağ, Kağan Huysal, Sevim Eşmedere Eren, published by Sciendo http://creativecommons.org/licenses/by-nc-nd/3.0 This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
spellingShingle Original Paper
Ustundağ, Yasemin
Huysal, Kağan
Eren, Sevim Eşmedere
Determining the Need for Repeat Testing of Blood Ethanol Concentration: Evaluation of the Synchron Blood Ethyl Alcohol Assay Kit
title Determining the Need for Repeat Testing of Blood Ethanol Concentration: Evaluation of the Synchron Blood Ethyl Alcohol Assay Kit
title_full Determining the Need for Repeat Testing of Blood Ethanol Concentration: Evaluation of the Synchron Blood Ethyl Alcohol Assay Kit
title_fullStr Determining the Need for Repeat Testing of Blood Ethanol Concentration: Evaluation of the Synchron Blood Ethyl Alcohol Assay Kit
title_full_unstemmed Determining the Need for Repeat Testing of Blood Ethanol Concentration: Evaluation of the Synchron Blood Ethyl Alcohol Assay Kit
title_short Determining the Need for Repeat Testing of Blood Ethanol Concentration: Evaluation of the Synchron Blood Ethyl Alcohol Assay Kit
title_sort determining the need for repeat testing of blood ethanol concentration: evaluation of the synchron blood ethyl alcohol assay kit
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6534961/
https://www.ncbi.nlm.nih.gov/pubmed/31156342
http://dx.doi.org/10.2478/jomb-2018-0032
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