Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Clonorchis sinensis Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens

We analyzed Clonorchis sinensis ancient DNA (aDNA) acquired from the specimens of the Joseon mummies. The target regions were cytochrome C oxidase subunit 1 (CO1), internal transcribed spacer 1 (ITS1), nicotinamide adenine dinucleotide hydrogen (NADH) dehydrogenase subunits 2 (NAD2) and 5 (NAD5). Th...

Descripción completa

Detalles Bibliográficos
Autores principales: Hong, Jong Ha, Oh, Chang Seok, Chai, Jong-Yil, Seo, Min, Shin, Dong Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Medical Sciences 2019
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6535405/
https://www.ncbi.nlm.nih.gov/pubmed/31124326
http://dx.doi.org/10.3346/jkms.2019.34.e149
_version_ 1783421563360509952
author Hong, Jong Ha
Oh, Chang Seok
Chai, Jong-Yil
Seo, Min
Shin, Dong Hoon
author_facet Hong, Jong Ha
Oh, Chang Seok
Chai, Jong-Yil
Seo, Min
Shin, Dong Hoon
author_sort Hong, Jong Ha
collection PubMed
description We analyzed Clonorchis sinensis ancient DNA (aDNA) acquired from the specimens of the Joseon mummies. The target regions were cytochrome C oxidase subunit 1 (CO1), internal transcribed spacer 1 (ITS1), nicotinamide adenine dinucleotide hydrogen (NADH) dehydrogenase subunits 2 (NAD2) and 5 (NAD5). The sequences of C. sinensis aDNA was completely or almost identical to modern C. sinensis sequences in GenBank. We also found that ITS1, NAD2 and NAD5 could be good markers for molecular diagnosis between C. sinensis and the other trematode parasite species. The current result could improve our knowledge about genetic history of C. sinensis.
format Online
Article
Text
id pubmed-6535405
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The Korean Academy of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-65354052019-05-28 Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Clonorchis sinensis Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens Hong, Jong Ha Oh, Chang Seok Chai, Jong-Yil Seo, Min Shin, Dong Hoon J Korean Med Sci Brief Communication We analyzed Clonorchis sinensis ancient DNA (aDNA) acquired from the specimens of the Joseon mummies. The target regions were cytochrome C oxidase subunit 1 (CO1), internal transcribed spacer 1 (ITS1), nicotinamide adenine dinucleotide hydrogen (NADH) dehydrogenase subunits 2 (NAD2) and 5 (NAD5). The sequences of C. sinensis aDNA was completely or almost identical to modern C. sinensis sequences in GenBank. We also found that ITS1, NAD2 and NAD5 could be good markers for molecular diagnosis between C. sinensis and the other trematode parasite species. The current result could improve our knowledge about genetic history of C. sinensis. The Korean Academy of Medical Sciences 2019-05-14 /pmc/articles/PMC6535405/ /pubmed/31124326 http://dx.doi.org/10.3346/jkms.2019.34.e149 Text en © 2019 The Korean Academy of Medical Sciences. https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Hong, Jong Ha
Oh, Chang Seok
Chai, Jong-Yil
Seo, Min
Shin, Dong Hoon
Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Clonorchis sinensis Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens
title Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Clonorchis sinensis Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens
title_full Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Clonorchis sinensis Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens
title_fullStr Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Clonorchis sinensis Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens
title_full_unstemmed Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Clonorchis sinensis Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens
title_short Cytochrome C Oxidase Subunit 1, Internal Transcribed Spacer 1, Nicotinamide Adenine Dinucleotide Hydrogen Dehydrogenase Subunits 2 and 5 of Clonorchis sinensis Ancient DNA Retrieved from Joseon Dynasty Mummy Specimens
title_sort cytochrome c oxidase subunit 1, internal transcribed spacer 1, nicotinamide adenine dinucleotide hydrogen dehydrogenase subunits 2 and 5 of clonorchis sinensis ancient dna retrieved from joseon dynasty mummy specimens
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6535405/
https://www.ncbi.nlm.nih.gov/pubmed/31124326
http://dx.doi.org/10.3346/jkms.2019.34.e149
work_keys_str_mv AT hongjongha cytochromecoxidasesubunit1internaltranscribedspacer1nicotinamideadeninedinucleotidehydrogendehydrogenasesubunits2and5ofclonorchissinensisancientdnaretrievedfromjoseondynastymummyspecimens
AT ohchangseok cytochromecoxidasesubunit1internaltranscribedspacer1nicotinamideadeninedinucleotidehydrogendehydrogenasesubunits2and5ofclonorchissinensisancientdnaretrievedfromjoseondynastymummyspecimens
AT chaijongyil cytochromecoxidasesubunit1internaltranscribedspacer1nicotinamideadeninedinucleotidehydrogendehydrogenasesubunits2and5ofclonorchissinensisancientdnaretrievedfromjoseondynastymummyspecimens
AT seomin cytochromecoxidasesubunit1internaltranscribedspacer1nicotinamideadeninedinucleotidehydrogendehydrogenasesubunits2and5ofclonorchissinensisancientdnaretrievedfromjoseondynastymummyspecimens
AT shindonghoon cytochromecoxidasesubunit1internaltranscribedspacer1nicotinamideadeninedinucleotidehydrogendehydrogenasesubunits2and5ofclonorchissinensisancientdnaretrievedfromjoseondynastymummyspecimens

Ejemplares similares