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Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster

The fourth chromosome smallest in the genome of Drosophila melanogaster differs from other chromosomes in many ways. It has high repeat density in conditions of a large number of active genes. Gray bands represent a significant part of this polytene chromosome. Specific proteins including HP1a, POF,...

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Autores principales: Sidorenko, Darya S., Sidorenko, Ivan A., Zykova, Tatyana Yu., Goncharov, Fedor P., Larsson, Jan, Zhimulev, Igor F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536484/
https://www.ncbi.nlm.nih.gov/pubmed/31041520
http://dx.doi.org/10.1007/s00412-019-00703-x
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author Sidorenko, Darya S.
Sidorenko, Ivan A.
Zykova, Tatyana Yu.
Goncharov, Fedor P.
Larsson, Jan
Zhimulev, Igor F.
author_facet Sidorenko, Darya S.
Sidorenko, Ivan A.
Zykova, Tatyana Yu.
Goncharov, Fedor P.
Larsson, Jan
Zhimulev, Igor F.
author_sort Sidorenko, Darya S.
collection PubMed
description The fourth chromosome smallest in the genome of Drosophila melanogaster differs from other chromosomes in many ways. It has high repeat density in conditions of a large number of active genes. Gray bands represent a significant part of this polytene chromosome. Specific proteins including HP1a, POF, and dSETDB1 establish the epigenetic state of this unique chromatin domain. In order to compare maps of localization of genes, bands, and chromatin types of the fourth chromosome, we performed FISH analysis of 38 probes chosen according to the model of four chromatin types. It allowed clarifying the dot chromosome cytological map consisting of 16 loose gray bands, 11 dense black bands, and 26 interbands. We described the relation between chromatin states and bands. Open aquamarine chromatin mostly corresponds to interbands and it contains 5′UTRs of housekeeping genes. Their coding parts are embedded in gray bands substantially composed of lazurite chromatin of intermediate compaction. Polygenic black bands contain most of dense ruby chromatin, and also some malachite and lazurite. Having an accurate map of the fourth chromosome bands and its correspondence to physical map, we found that DNase I hypersensitivity sites, ORC2 protein, and P–elements are mainly located in open aquamarine chromatin, while element 1360, characteristic of the fourth chromosome, occupies band chromatin types. POF and HP1a proteins providing special organization of this chromosome are mostly located in aquamarine and lazurite chromatin. In general, band organization of the fourth chromosome shares the features of the whole Drosophila genome. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00412-019-00703-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-65364842019-06-12 Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster Sidorenko, Darya S. Sidorenko, Ivan A. Zykova, Tatyana Yu. Goncharov, Fedor P. Larsson, Jan Zhimulev, Igor F. Chromosoma Original Article The fourth chromosome smallest in the genome of Drosophila melanogaster differs from other chromosomes in many ways. It has high repeat density in conditions of a large number of active genes. Gray bands represent a significant part of this polytene chromosome. Specific proteins including HP1a, POF, and dSETDB1 establish the epigenetic state of this unique chromatin domain. In order to compare maps of localization of genes, bands, and chromatin types of the fourth chromosome, we performed FISH analysis of 38 probes chosen according to the model of four chromatin types. It allowed clarifying the dot chromosome cytological map consisting of 16 loose gray bands, 11 dense black bands, and 26 interbands. We described the relation between chromatin states and bands. Open aquamarine chromatin mostly corresponds to interbands and it contains 5′UTRs of housekeeping genes. Their coding parts are embedded in gray bands substantially composed of lazurite chromatin of intermediate compaction. Polygenic black bands contain most of dense ruby chromatin, and also some malachite and lazurite. Having an accurate map of the fourth chromosome bands and its correspondence to physical map, we found that DNase I hypersensitivity sites, ORC2 protein, and P–elements are mainly located in open aquamarine chromatin, while element 1360, characteristic of the fourth chromosome, occupies band chromatin types. POF and HP1a proteins providing special organization of this chromosome are mostly located in aquamarine and lazurite chromatin. In general, band organization of the fourth chromosome shares the features of the whole Drosophila genome. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00412-019-00703-x) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2019-04-30 2019 /pmc/articles/PMC6536484/ /pubmed/31041520 http://dx.doi.org/10.1007/s00412-019-00703-x Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Sidorenko, Darya S.
Sidorenko, Ivan A.
Zykova, Tatyana Yu.
Goncharov, Fedor P.
Larsson, Jan
Zhimulev, Igor F.
Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster
title Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster
title_full Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster
title_fullStr Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster
title_full_unstemmed Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster
title_short Molecular and genetic organization of bands and interbands in the dot chromosome of Drosophila melanogaster
title_sort molecular and genetic organization of bands and interbands in the dot chromosome of drosophila melanogaster
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536484/
https://www.ncbi.nlm.nih.gov/pubmed/31041520
http://dx.doi.org/10.1007/s00412-019-00703-x
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