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Quantification of very low-abundant proteins in bacteria using the HaloTag and epi-fluorescence microscopy
Cell biology is increasingly dependent on quantitative methods resulting in the need for microscopic labelling technologies that are highly sensitive and specific. Whilst the use of fluorescent proteins has led to major advances, they also suffer from their relatively low brightness and photo-stabil...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6536506/ https://www.ncbi.nlm.nih.gov/pubmed/31133640 http://dx.doi.org/10.1038/s41598-019-44278-0 |